Cloning and expression of rat CYP2E1 in Saccharomyces cerevisiae: Detection of genotoxicity of N-alkylformamides

Environmental and Molecular Mutagenesis

Volumn 36, Issue 2, 2000, Pages 97-104

  Aryal, Pramod ^a,b   Terashita, Takao ^b   Peter Guengerich, F ^c   Shimada, Tsutomu ^a   Oda, Yoshimitsu ^a  

^a OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH   (Japan)

^b KINKI UNIVERSITY   (Japan)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cytochrome P450 2E1; Genotoxicity; Hepatotoxic solvents; Metabolism; Microsomes; Saccharomyces cerevisiae

Indexed keywords

COMPLEMENTARY DNA; CYTOCHROME P450 REDUCTASE; DIMETHYLNITROSAMINE DEMETHYLASE; FORMAMIDE DERIVATIVE; N ETHYLFORMAMIDE; N METHYLFORMAMIDE; PYRAZOLE DERIVATIVE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENOTOXICITY; LIVER CARCINOGENESIS; LIVER MICROSOME; LIVER TOXICITY; MALE; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; RAT; SACCHAROMYCES CEREVISIAE;

ANIMAL; BIOTRANSFORMATION; CLONING, MOLECULAR; CYTOCHROME P-450 CYP2E1; FORMAMIDES; GENETIC VECTORS; MALE; NITROSO COMPOUNDS; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; TOXICITY TESTS;

EID: 0033775786     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/1098-2280(2000)36:2<97::AID-EM3>3.0.CO;2-4     Document Type: Article

References (45)

1. Microsomal oxidation of N,N-diethylformamide and its effect on P450-dependent monooxygenases in rat liver
2. Yeast cytochrome P-450 catalyzing lanosterol 14 alpha-demethylation. II. Lanosterol metabolism by purified P-450(14)DM and by intact microsomes
3. The expression of cytochrome P450IIB1 in Saccharomyces cerevisiae results in an increased mutation frequency when exposed to cyclophosphamide
4. Evaluation of the yeast DEL assay with 10 compounds selected by the International Program on Chemical Safety for the evaluation of short-term test for carcinogens
5. Cytochrome P-450, factors determining synthesis in strain D7 of S. cerevisiae
6. Specific inhibitors of the monooxygenase system of S. cerevisiae modified the mutagenic effect of 4-nitroquinoline-1-oxide and the deethylation activity
7. Coexpression of mammalian cytochrome P450 and reductase in Escherichia coli
8. Constitutive and inducible expression of human cytochrome P4501A1 in yeast Saccharomyces cerevisiae: An alternative enzyme source for in vitro studies
9. Increased sensitivity of the microsomal oxidation of ethanol to inhibition by pyrazole and 4-methylpyrazole after chronic ethanol treatment
10. Expression of alcohol-inducible rabbit liver cytochrome P-459 3a (P-450IIE1) in Saccharomyces cerevisiae with the copper-inducible CUP1 promoter
11. Cell division transforms mutagenic lesions into deletion-recombinagenic lesions in yeast cells
12. Engineered yeast cells as model to study coupling between human xenobiotic metabolizing enzymes: Simulation of the first two steps of benzo[a]pyrene activation
13. Metabolism of N,N-dimethylformamide: Key to the understanding of its toxicity
14. Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acid as carrier
15. Cytochromes P450 expression system
16. Microsomal enzymes involved in toxicology: Analysis and separation
17. Expression of mammalian cytochrome P-450 enzymes using yeast-based vector
18. Role of human cytochrome P4502E1 in the oxidation of many low molecular weight cancer suspects
19. New applications of bacterial system to problems in toxicology
20. Role of covalent and non covalent interaction in cell toxicity: Effect on proteins
21. Metabolic oxidation and toxification of N-methylformamide catalyzed by the cytochrome P-450 isoenzyme CYP2E1
22. Dimethylformamide
23. Multiple forms of human P450 expressed in Saccharomyces cerevisiae
24. Biological effect of acetamide, formamide and monomethyl and dimethyl derivates
25. Monoclonal antibodies to ethanol-induced rat liver cytochrome P-450 that metabolize aniline and nitrosamines
26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
27. N-nitrosodimethylamine-mediated cytotoxicity in a cell line expressing P450 2E1: Evidence for apoptotic cell death
28. Protein measurement with the Folin reagent
29. Microsomal metabolism of N,N-diethylacetamide and N,N-dimethylacetamide and their effects on drug-metabolizing enzymes of rat liver
30. Mitotic recombination in mammalian cells in vivo
31. Differences between rodents and humans in the metabolic toxification of N,N-dimethylformamide
32. Investigation of the mechanistic basis of N,N-dimethylformamide and its deuterated isotopomers by cytochrome P-450 2E1
33. Establishment of a novel host, high-red yeast that stably expresses hamster NADPH-cytochrome P450 oxidoreductase: Usefulness for examination of the function of mammalian Cyt. P450
34. The carbon-monoxide binding protein of liver microsomes: Evidence for its hemoprotein nature
35. Yeast expression of animal and plant P450s in optimized redox environments
36. Purification and characterization of an acetone-inducible cytochrome P-450 from hamster liver microsomes
37. Comparison of ethanol and imidaziole pretreatments on hepatic monooxygenase activities in the rat
38. Mitotic recombination in yeast
39. Nonmutagenic carcinogen induce intrachromosomal recombination in yeast
40. Analysis of the mechanism for reversion of a disrupted gene
41. Carcinogens induce intrachromosomal recombination in yeast
42. High promutagen activating capacity of yeast microsomes containing human cytochrome P4501A and human NADPH-cytochrome P450 reductase
43. Cytotoxicity and metabolism of the hepatotoxin N-methylformamide and related formamides in mouse hepatocytes
44. Expression and coupling of human cytochrome P450 2E1 and NADPH-cytochrome P450 oxidoreductase in dual expression and co-infection systems with baculovirus in insect cells
45. Primary structure of Saccharomyces cerevisiae NADPH-cytochrome P450 reductase deduced from nucleotide sequence of its cloned gene