Protein disulfide isomerase-mediated cell-free assembly of recombinant interleukin-12 p40 homodimers

European Journal of Biochemistry

Volumn 267, Issue 22, 2000, Pages 6679-6683

  Martens, Erik ^a   Alloza, Iraide ^b   Scott, Christopher J ^b   Billiau, Alfons ^a   Vandenbroeck, Koen ^a,b  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Cytokine; Dimer; Interleukin 12; Protein disulfide isomerase; Refolding

Indexed keywords

BOVINE SERUM ALBUMIN; BUFFER; CHAPERONE; CHAPERONIN; CYTOKINE; DIMER; GLUTATHIONE; GLUTATHIONE DISULFIDE; PROTEIN DISULFIDE ISOMERASE; PROTEIN DNAK; PROTEIN P35; PROTEIN P40; PROTEIN SUBUNIT; RECOMBINANT INTERLEUKIN 12; SOLVENT; UREA;

ARTICLE; CELL FREE SYSTEM; CELL INCLUSION; DIMERIZATION; DISULFIDE BOND; ESCHERICHIA COLI; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN FOLDING;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELL-FREE SYSTEM; CLONING, MOLECULAR; DIMERIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLUTATHIONE; GROEL PROTEIN; HEAT-SHOCK PROTEINS; HSP40 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; INCLUSION BODIES; INTERLEUKIN-12; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PROTEIN DISULFIDE-ISOMERASE; PROTEIN FOLDING; PROTEIN SUBUNITS; RECOMBINANT PROTEINS;

BOVINAE; ESCHERICHIA COLI;

EID: 0033773797     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01765.x     Document Type: Article

References (19)

1. The interleukin-12 subunit p40 specifically inhibits effects of the interleukin-12 heterodimer
2. Mouse interleukin-12 (IL-12) p40 homodimer - A potent IL-12 antagonist
3. Characterization of mouse interleukin-12 p40 homodimer binding to the interleukin-12 receptor subunits
4. Interleukin-12 antagonist activity of mouse interleukin-12 p40 homodimer in vitro and in vivo
5. Treatment with homodimeric interleukin-12 (IL-12) p40 homodimer protects mice from IL-12-dependent shock but not from tumor necrosis factor alpha-dependent shock
6. Local production of the p40 subunit of interleukin-12 suppresses T-helper 1-mediated immune responses and prevents allogeneic myoblast rejection
7. Increased interleukin-12 production in progressive multiple sclerosis: Induction by activated CD4 (+) T cells via CD40 ligand
8. Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin-12
9. Molecular cloning and mRNA expression of porcine interleukin-12
10. GroEL/ES chaperonins protect interferon-γ against physicochemical stress. Study of tertiary structure formation by α-casein quenching and ELISA
11. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
12. Refolding and single-step purification of porcine interferon-γ from Escherichia coli inclusion bodies. Conditions for reconstitution of dimeric IFN-γ
13. In vitro folding of inclusion body proteins
14. Renaturation of 1-aminocyclopropane-1-carboxylate synthase expressed in Escherichia coli in the form of inclusion bodies into a dimeric and catalytically active enzyme
15. A novel function of Escherichia coli chaperone DnaJ - Protein disulfide isomerase
16. 2
17. Study on the interactions between protein disulfide isomerase and target proteins using immobilization on solid support
18. Enzymes as chaperones and chaperones as enzymes
19. Corefolding denatured-reduced hen egg white lysozyme with acidic and basic proteins