Specificities of functionally expressed chalcone and acridone synthases from Ruta graveolens

European Journal of Biochemistry

Volumn 267, Issue 22, 2000, Pages 6552-6559

  Springob, Karin ^a   Lukacin, Richard ^a   Ernwein, Claudia ^a   Gröning, Inga ^a   Matern, Ulrich ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Acridone synthases; Chalcone synthases; Functional expression; Ruta graveolens; Substrate specificities

Indexed keywords

ACRIDONE DERIVATIVE; CHALCONE; COMPLEMENTARY DNA; FLAVANONE; FLAVONOID; MALONYL COENZYME A; RUTA GRAVEOLENS EXTRACT; SYNTHETASE;

ARTICLE; CITRUS FRUIT; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; CITRUS; CLONING, MOLECULAR; GENES, PLANT; KINETICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; ROSALES; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

CITRUS; CITRUS SINENSIS; ESCHERICHIA COLI; RUTA; RUTA GRAVEOLENS;

EID: 0033773791     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01746.x     Document Type: Article

References (39)

1. Molecular cloning and heterologous expression of acridone synthase from elicited Ruta graveolens L. cell suspension cultures
2. Antitumor activity and murine pharmacokinetics of parenteral acronycine
3. Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L
4. Synthesis of 1,3-dihydroxy-N-methylacridone and its conversion to rutacridone by cell-free extracts of Ruta graveolens cell cultures
5. Identification of two gossypetin monomethyl ethers as yellow flower pigments in the Rutaceae
6. Biosynthesis of rutacridone in tissue cultures of Ruta graveolens L
7. Increased accumulation of acridone alkaloids by cell suspension cultures of Ruta graveolens in response to elicitors
8. Differential regulation and distribution of acridone synthase in Ruta graveolens
9. Native acridone synthases I and II from Ruta graveolens L. form homodimers
10. The role of cysteines in polyketide synthases: Site-directed mutagenesis of resveratrol and chalcone synthase, two key enzymes in different plant-specific pathways
11. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis
12. Dissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthase
13. A family of plant-specific polyketide synthases: Facts and predictions
14. Evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution
15. Identification of strictly conserved histidine and arginine residues as part of the active site in Petunia hybrida flavanone 3β-hydroxylase
16. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acid
17. Molecular analysis of resveratrol synthase, cDNA, genomic clones and relationship with chalcone synthase
18. DNA sequencing with chain-terminating inhibitors
19. Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing
20. Rapid and efficient site-specific mutagenesis without phenotypic selection
21. Rapid and efficient site-specific mutagenesis without phenotypic selection
22. Purification of recombinant flavanone 3β-hydroxylase from Petunia hybrida and assignment of the primary site of proteolytic degradation
23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
24. Protein measurement with the Folin phenol regent
25. Molecular analysis of chalcone and dihydropinosylvin synthases from Scotch pine (Pinus sylvestris), and differential regulation of these and related enzyme activities in stressed plants
26. A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack
27. Enzymatic synthesis of aromatic compounds in higher plants. Formation of bis-noryangonin (4-hydroxy-6[4-hydroxystyryl]2-pyrone) from p-coumaroyl-CoA and malonyl-CoA
28. Cross-reaction of chalcone synthase and stilbene synthase overexpressed in Escherichia coli
29. Purification and characterization of (2S)-flavanone 3-hydroxylase from Petunia hybrida
30. Purification and properties of a stilbene synthase from induced cell suspension cultures of peanut
31. Benzophenone synthase from cultured cells of Centaurium erythraea
32. Plant polyketide synthases: A chalcone synthase-type enzyme which performs a condensation reaction with methyl-malonyl-CoA in the biosynthesis of C-methylated chalcones
33. The chalcone-stilbene synthase-type family of condensing enzymes
34. Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida
35. Organization of soybean chalcone synthase gene clusters and characterization of a new member of the family
36. Molecular evolution of the chalcone synthase multigene family in the morning glory genome
37. New pathway to polyketides in plants
38. Cloning and expression of cytochrome P450 genes controlling flower colour