Characterization of the internal motions of a chimeric protein by 13C NMR highlights the important dynamic consequences of the engineering on a millisecond time scale

European Journal of Biochemistry

Volumn 267, Issue 22, 2000, Pages 6519-6533

  Wolff, Nicolas ^a   Guenneugues, Marc ^a   Gilquin, Bernard ^a   Drakopoulou, Eugenia ^a   Vita, Claudio ^a   Ménez, André ^a   Zinn Justin, Sophie ^a  

^a DIF   (France)

Author keywords

Internal dynamics; Microsecond to millisecond time scale motion; Protein engineering; Scorpion fold; Three dimensional structure

Indexed keywords

ALPHA TOXIN; CHARYBDOTOXIN; CHIMERIC PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; DYNAMICS; GENE DELETION; GENE MUTATION; GENETIC ENGINEERING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; CHARYBDOTOXIN; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDE FRAGMENTS; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; SCORPION VENOMS; SCORPIONS; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SNAKE VENOMS;

SCORPIONES; SERPENTES;

EID: 0033773436     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01723.x     Document Type: Article

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