Real-time studies of zymogen granule exocytosis in intact rat pancreatic acinar cells

Journal of Physiology

Volumn 528, Issue 2, 2000, Pages 317-326

  Campos Toimil, M ^a   Edwardson, J M ^a   Thomas, P ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; CALCIUM ION; FURA 2; MEPACRINE; ZYMOGEN GRANULE; PROTEIN KINASE C;

ACINAR CELL; ANIMAL CELL; ARTICLE; BASAL METABOLIC RATE; CALCIUM CELL LEVEL; CELL ACTIVATION; CELL KINETICS; CELL METABOLISM; CELL SECRETION; CONTROLLED STUDY; EXOCYTOSIS; FLUORESCENCE; IMAGING SYSTEM; MALE; NONHUMAN; PANCREAS CELL; PRIORITY JOURNAL; RAT; SECOND MESSENGER; ACTION POTENTIAL AMPLITUDE; CELL MEMBRANE CONDUCTANCE; CELL VACUOLE; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME RELEASE; ENZYME SYNTHESIS; INCUBATION TIME; MOLECULAR IMAGING; PROTEIN SECRETION;

EID: 0033761036     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-7793.2000.00317.x     Document Type: Article

References (50)

1. Fluorescence microscopy in three dimensions
2. Correlative morphometric and biochemical study on pancreatic amylase in normal and streptozotocin-diabetic rats
3. Calcium requirements for secretion in bovine chromaffin cells
4. Imaging exocytosis and endocytosis
5. Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction
6. Final steps in exocytosis observed in a cell with giant secretory granules
7. 2+ current run-down
8. Simultaneous monitoring of eytosolic free calcium and exocytosis at the single cell level
9. Zymogen granules of pancreas decrease in size in response to feeding
10. Zymogen granule size in pancreas of nursing rats
11. Capacitance measurements reveal stepwise fusion events in degranulating mast cells
12. Optical measurement of stimulus-evoked membrane dynamics in single pancreatic acinar cells
13. Phospholipid turnover in isolated rat pancreatic acini
14. Calcium dependence of the rate of exocytosis in a synaptic terminal
15. 2+
16. Synthesis, intracellular transport, and discharge of secretory proteins in stimulated pancreatic exocrine cells
17. Redistribution of a rab3-like GTP-binding protein from secretory granules to the Golgi complex in pancreatic acinar cells during regulated exocytosis
18. Neurotransmitter-induced exocytosis in goblet and acinar cells of rat nasal mucosa studied by video microscopy
19. 2+ -dependent exocytosis: Implications of kinetic diversity for secretory function
20. Agonist-induced changes in cell membrane capacitance and conductance in dialysed pancreatic acinar cells of rats
21. 2+ spikes in mouse pancreatic acinar cells
22. Pancreatic amylase secretion and cytoplasmic free calcium
23. Activation of nicotinic receptors triggers exocytosis from bovine chromaffin cells in the absence of membrane depolarization
24. Actin filament disassembly is a sufficient final trigger for exocytosis in nonexcitable cells
25. Discrete changes of cell membrane capacitance observed under condition of enhanced secretion in bovine adrenal chromaffin cells
26. Kinetic diversity in the fusion of exocytotic vesicles
27. Heterotrimeric G-protein G(q)/11 localized on pancreatic zymogen granules is involved in calcium-regulated amylase secretion
28. 2+ to prime amylase secretion from permeabilized rat pancreatic acinar acini
29. The two phases of regulated exocytosis in permeabilized pancreatic acini are modulated differently by heterotrimeric G-proteins
30. Intracellular aspects of the process of protein synthesis
31. Role of free cytosolic calcium in secretagogue-stimulated amylase release from dispersed acini from guinea pig pancreas
32. Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells
33. Cyclic GMP inhibits protein kinase C-mediated secretion in rat pancreatic acini
34. A role for the p38 mitogen-activated protein kinase/HSP27 pathway in cholecystokinin-induced changes in the actin cytoskeleton in rat pancreatic acini
35. Different time courses of GTP[γ-S]- induced exocytosis and current oscillations in isolated mouse pancreatic acinar cells
36. Exocytosis in living salivary glands: Direct visualization by video-enhanced microscopy and confocal laser microscopy
37. Kinetics of stimulus-coupled secretion in dialyzed bovine chromaffin cells in response to trains of depolarizing pulses
38. Studies on the pancreas of the guinea pig. Parallel processing and discharge of exocrine proteins
39. Monitoring exocytosis from single mast cells by fast voltammetry
40. Quantitative analysis of exocytosis directly visualized in living chromaffin cells
41. Two types of exocytosis observed in single, rat pancreatic acinar cells
42. The LβT2 clonal gonadotrope: A model for single cell studies of endocrine cell secretion
43. 2+, exocytosis and endocytosis in single melanotrophs of the rat pituitary
44. Modulation of stimulus-secretion coupling in single rat gonadotrophs
45. 2+ receptor controls the final steps in peptide secretion from pituitary melanotrophs
46. 2+ spiking in pancreatic acinar cells
47. Rhythmic exocytosis stimulated by GnRH-induced calcium oscillations in rat gonadotropes
48. Role of actin in regulated exocytosis and compensatory membrane retrieval: Insights from an old acquaintance
49. Chromaffin cell cortical actin network dynamics control the size of the release-ready vesicle pool and the initial rate of exocytosis
50. Simulataneous electrical and optical measurements show that membrane fusion precedes secretory granule swelling during exocytosis of beige mouse mast cells