Escherichia coli DNA polymerase III τ- and γ-subunit conserved residues required for activity in vivo and in vitro

Journal of Bacteriology

Volumn 182, Issue 21, 2000, Pages 6106-6113

  Walker, J R ^a   Hervas, C ^a   Ross, J D ^a   Blinkova, A ^a   Walbridge, M J ^a   Pumarega, E J ^a   Park, M O ^a   Neely, H R ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED DNA POLYMERASE GAMMA;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE MUTATION; GENETIC COMPLEMENTATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATASES; BACTERIAL PROTEINS; DNA POLYMERASE III; ESCHERICHIA COLI; MUTATION;

ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA; PROKARYOTA;

EID: 0033759203     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.21.6106-6113.2000     Document Type: Article

References (78)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. Pre-steady state analysis of the assembly of wild type and mutant circular clamps of Escherichia coli DNA polymerase III onto DNA
3. Programmed ribosome frameshift generates the Escherichia coli DNA polymerase III γ subunit from within the τ subunit reading frame
4. The Escherichia coli DNA polymerase III holoenzyme contains both products of the dnaX gene, τ and γ, but only τ is essential
5. Dynamics of loading the β sliding clamp of DNA polymerase III onto DNA
6. An expanding family of helicases within the 'DEAD/H' superfamily
7. Saccharomyces cerevisiae replication factor C. II. Formation and activity of complexes with the proliferating cell nuclear antigen and with DNA polymerases δ and ε
8. Identification, isolation, and characterization of the strucutural gene encoding the δ' subunit of Eschedchia coli DNA polymerase III hotoenzyme
9. Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at non-permissive temperatures
10. Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes
11. Characterization of the five replication factor C genes of Saccharomyces cerevisiae
12. DnaX complex of DNA polymerase III holoenzyme. Physical characterization of the DnaX subunits and complexes
13. DnaX complex of Escherichia coli DNA polymerase Ill holoenzyme. Centrol role of τ in initiation complex assembly and in determining the functional asymmetry of holoenzyme
14. DNA polymerase III accessory proteins. I holA and holB encoding δ and δ'
15. Refined structure of porcine cytosolic adenylate kinase at 2.1 Å resolution
16. 5-di(adenosine-5'-) pentaphosphate at 2.6 Å resolution
17. Identification of replication factor C from Saccharomyces cerevisiae: A component of the leading-strand DNA replication complex
18. Bacterial cell division regulation: Characterization of the dnaH locus of Escherichia coli
19. The γ subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshift
20. Prereplicative complexes of components of DNA polymerase III holoenzyme of Escherichia coli
21. Identification of the fifth subunit of Saccharomyces cerevisiae replication factor C
22. Two related superfamilies of putative helicases involved in replication, recombination, repair, and expression of DNA and RNA genomes
23. Crystal structure of the δ' subunit of the clamp-loader complex of E. coli DNA polymerase III
24. New method for generating deletions and gene replacements in Escherichia coli
25. Amino acid substitution matrices from protein blocks
26. ATP binding to the Escherichia coli clamp loader powers opening of the ring-shaped clamp of DNA polymerase IIl holoenzyme
27. Division of labor - Sequential ATP hydrolysis drives assembly of a DNA polymerase sliding clamp around DNA
28. A new family of replicative proteins
29. Structure of the GDP domain of EF-Tu and location of the amino acids homologous to ras oncogene proteins
30. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions
31. Biotin tagging deletion analysis of domain limits involved in protein-macromolecular interactions. Mapping the τ binding domain of the DNA polymerase III α subunit
32. Coupling of a replicative polymerase and a helicase: A τ-DnaB interaction mediates rapid replication fork movement
33. τ protects β in the leading-strand polymerase complex at the replication fork
34. τ couples the leading- and lagging-strand polymerases at the Escherichia coli DNA replication fork
35. Similarities in RNA hellcases
36. Rapid and efficient site-specific mutagenesis without phenotypic selection
37. Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X-ray crystallography
38. Streptomycin resistance: A genetically recessive mutation
39. Mechanism of elongation of primed DNA by DNA polymerase δ, proliferating cell nuclear antigen, and activator 1
40. Studies on the activator 1 protein complex, an accessory factor for proliferating cell nuclear antigen-dependent DNA polymerase δ
41. Escherichia coli DnaX product, the τ subunit of DNA polymerase III, is a multifunctional protein with single-stranded DNA-dependent ATPase activity
42. Sequence and analysis of chromosome II of Arabidopsis thaliana
43. Identification and characterization of a cyanobacterial DnaX intein
44. DNA polymerase III holoenzyme of Escherichia coli. I. Purification and distinctive functions of subunits τ and γ, the dnaZX gene products
45. DNA polymerase III holoenzyme of Escherichia coli. II. A novel complex including the γ subunit essential for processive synthesis
46. Purification and characterization of DNA polymerase III': Identification of τ as a subunit of the DNA polymerase III holoenzyme
47. DNA polymerase III of Escherichia coli. Purification and resolution into subunits
48. A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
49. A molecular switch in a replication machine defined by an internal competition for protein rings
50. Homology in accessory proteins of replicative polymerases - E. coli to humans
51. Accessory proteins bind a primed template and mediate rapid cycling of DNA polymerase III holoenzyme from Escherichia coli
52. Assembly of a chromosomal replication machine: Two DNA polymerases, a clamp loader and sliding clamps in one holoenzyme particle. I. Organization of the clamp loader
53. NA polymerase III accessory proteins. II. Characterization of δ and δ'
54. Analysis of the ATPase subassembly which initiates processive DNA synthesis by DNA polymerase III holoenzyme
55. Refined structure of the triphosphate conformation of H-ras-p21 at 1.35 Å resolution: Implications for the mechanism of GTP hydrolysis
56. Mutational analysis of a DEAD box RNA helicase: The mammalian translation initiation factor elF-4A
57. In vivo assembly of the τ-complex of the DNA polymerase III holoenzyme expressed from a five-gene artificial operon. Cleavage of the τ-complex to form a mixed γ-τ-complex by the OmpT protease
58. DEAD protein family of putative RNA helicases
59. Positive selection vectors for allelic exchange
60. Use of T7 RNA polymerase to direct expression of cloned genes
61. Processive replication is contingent on the exonuclease subunit of DNA polymerase III holoenzyme
62. Constitution of twin polymerase of DNA polymerase III holoenzyme
63. Mechanism of the sliding β clamp of DNA polymerase III holoenzyme
64. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
65. Structure of ras proteins
66. ATP interactions of the τ and γ subunits of DNA polymerase III holoenzyme of Escherichia coli
67. Translational frameshifting generates the y subunit of DNA polymerase III holoenzyme
68. Multiple replication factors augment DNA synthesis by the two eukaryotic DNA polymerases α and δ
69. Purification of a cellular replication factor, RF-C, that is required for coordinated synthesis of leading and lagging strands during simian virus 40 DNA replication in vitro
70. Replication factors required for SV40 DNA replication in vitro. I. DNA structure-specfic recognition of a primer-template junction by eukaryotic DNA polymerases and their accessory proteins
71. The internal workings of a DNA polymerase clamp-loading machine
72. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
73. A new form of DNA polymerase III and a copolymerase replicate a long single-stranded primer template
74. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli
75. Translation of the leaderless Caulobacter dnaX mRNA
76. Assembly of chromosomal replication machine: Two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. IV. ATP-binding site mutants identify the clamp loader
77. Replisome assembly reveals the basis for symmetric function in leading and lagging strand replication