Monolayer anulus fibrosus cell cultures in a mechanically active environment: Local culture condition adaptations and cell phenotype study

Journal of Laboratory and Clinical Medicine

Volumn 136, Issue 5, 2000, Pages 412-421

  Rannou, François ^a,b   Poiraudeau, Serge ^a,b   Foltz, Violaine ^a,b   Boiteux, Michel ^a,b   Corvol, Maïté ^a,b   Revel, Michel ^a,b  

^a INSERM   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANULUS FIBROSUS REGION; CELL AGGREGATION; CELL CULTURE; CULTURE MEDIUM; ENVIRONMENT; INTERVERTEBRAL DISK; MESSENGER RNA; PHENOTYPE; PROTEOGLYCAN; RABBIT;

EID: 0033747987     PISSN: 00222143     EISSN: None     Source Type: Journal    
DOI: 10.1067/mlc.2000.109755     Document Type: Article

References (42)

1. Turbulent fluid shear stress induces endothelial cell turnover in vitro
2. Increased calcification of growth plate cartilage as a result of compressive force in vitro
3. Effect of wheat germ agglutinin on the viscoelastic properties of erythrocyte membrane
4. A new vacuum-operated stress providing instrument that applies static or variable duration cyclic tension or compression to cells in vitro
5. Culturing cells in a mechanically active environment
6. The proliferative and synthetic response of isolated calvarial bone cells of rats to cyclic biaxial mechanical strain
7. In vitro response of chondrocytes to mechanical loading: The effect of short term mechanical tension
8. Cyclic stretching stimulates synthesis of matrix components by arterial smooth muscle cells in vitro
9. Implications of pulsatile stretch on growth of saphenous vein and mammary artery smooth muscle
10. Modulation of endothelial cell phenotype by cyclic stretch: Inhibition of collagen production
11. Enhanced collagen production by smooth muscle cells during repetitive mechanical sretching
12. Effects of repetitive motion on human fibroblasts
13. 2
14. Cyclic tensile stretch on bovine articular chondrocytes inhibits proteine kinase C activity
15. Cyclic tensile stress exerts antiinflammatory actions on chondrocytes by inhibiting inducible nitric oxide synthase
16. Cyclic mechanical stretch stress increases the growth rate and collagen synthesis of nucleus pulposus cells
17. Further observations on the elastic mechanism of the intervertebral disc
18. Observations on the postnatal structure of the intervertebral disc in man
19. In vivo measurements of intradical pressure
20. The collagenous changes in the intervertebral disc with age and their effects on its elasticity, an X-ray crystallographic study
21. On the mechanical behaviour of intervertebral discs
22. Radial bulging of the anulus fibrosus during compression of the intervertebral disc
23. Physical properties and functional biomechanics of the spine
24. Surface strain on human intervertebral discs
25. A new culture system to study the metabolism of the intervertebral disc in vitro
26. Initial characterization of the metabolism of intervertebral disc cells encapsulated in microspheres
27. Phenotypic characteristics of rabbit intervertebral disc cells: Comparison with cartilage cells from the same animals
28. Sensitivity of anulus fibrosus cells to IL1-comparison with articular chondrocytes
29. Evidence for a direct in vitro action of sex steroids on rabbit cartilage cells during skeletal growth: Influence of age and sex
30. Strain profiles for circular cell culture plates containing flexible surfaces employed to mechanically deform cells in vitro
31. Tensile properties of nondegenerate human lumbar anulus fibrosus
32. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction
33. Isolation and partial characterization of the entire human pro alpha 1 (II) collagen gene
34. Cloning and characterisation of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain
35. In vitro biosynthesis by articular chondrocytes of specific low molecular size proteoglycan pool
36. Modulation of the chondrocyte phenotype in vitro
37. Metabolism of the extracellular matrix formed by intervertebral disc cells cultured in alginate
38. Suppression of proteoglycan synthesis in chondrocyte cultures derived from canine intervertebral disc
39. Stretch affects phenotype and proliferation of vascular smooth muscle cells
40. Role of epidermal growth factor and its receptor in mechanical stress-induced differentiation of human periodontal ligament cells in vitro
41. Osteoclastogenesis is repressed by mechanical strain in an in vitro model
42. Mechanoregulation of chondrocyte proliferation, maturation, and hypertrophy: Ion-channel dependent transduction of matrix deformation signals