A novel approach to the quantification of hepatic stellate cells in intravital fluorescence microscopy of the liver using a computerized image analysis system

Microvascular Research

Volumn 60, Issue 3, 2000, Pages 232-240

  Zhang, X Y ^a   Sun, C K ^a   Wheatley, A M ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Hepatic stellate cells; In vivo microscopy; NIH image; Rat liver microcirculation

Indexed keywords

RETINOL;

ALGORITHM; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTOFLUORESCENCE; AUTOMATION; CELL COUNT; CELL DENSITY; COMPUTER SYSTEM; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; LIVER CELL; LIVER CIRCULATION; MALE; MICROCIRCULATION; NONHUMAN; PRACTICE GUIDELINE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; STELLATE CELL; VIDEORECORDING;

EID: 0033746528     PISSN: 00262862     EISSN: None     Source Type: Journal    
DOI: 10.1006/mvre.2000.2264     Document Type: Article

References (27)

1. Computer quantification of intracellular gold particles
2. Distribution of organella and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A sereological study
3. Remodeling of hepatic microvascular responsiveness after ischemia/reperfusion
4. α-Smooth muscle actin-positive peri-sinusoidal stromal cells in human hepatocellular carcinoma
5. Molecular mechanisms of hepatic fibrosis and principles of therapy
6. Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies
7. Point counting on the Macintosh. A semiautomated image analysis technique
8. A standardized, a computer-assisted in vitro assay for the assessment of neutrophil transmigration across endothelial monolayers
9. The hepatic stellate (Ito) cell: Its role in human liver disease
10. Endothelin receptors in rat liver: Lipocytes as a contractile target for endothelin 1
11. Optimization of single-cell gel electrophoresis (SCGE) for quantitative analysis of neuronal DNA damage
12. A comparison of the cutaneous microvascular properties of the spontaneously hypertensive rat and the Wistar-Kyoto rat
13. Endothelin induced contractility of stellate cells from normal and cirrhotic rat liver: Implications for regulation of portal pressure and resistance
14. Biomedical image processing
15. Carbon monoxide as an endogenous modulator of hepatic vascular perfusion
16. Intravital and electron microscopic observation of Ito cells in rat hepatic microcirculation
17. Topographic dissociation between mitochondrial dysfunction and cell death during low-flow hypoxia in perfused rat liver
18. Prostaglandin E1 abrogates early reductive stress and zone-specific paradoxidal oxidative injury in hypoperfused rat liver
19. A correlation of intravital microscopically assessed NADH fluorescence, tissue oxygenation, and organ function during shock and resuscitation of the rat liver
20. A new method for the intravital microscopic quantification of hepatic sinusoidal perfusion failure using the dye bisbenzamide H33342
21. An intravital fluorescence microscopic study of hepatic microvascular and cellular derangements in developing cirrhosis in rats
22. Lymph vessel expansion and function in the development of hepatic fibrosis and cirrhosis
23. Biosynthesis and degradation of hyaluronan by nonparenchymal liver cells during liver regeneration
24. Light microscopic image analysis system to quantify immunoreactive terminal area apposed to nerve cells
25. Vessel- and target cell-specific actions of endothelin-1 and endothelin-3 in rat liver
26. Endothelin-1 induces direct constriction of hepatic sinusoids
27. Morphometry of the liver after liver transplantation in the rat: Significance of an intact arterial supply