Surface expression of O-specific lipopolysaccharide in Escherichia coli requires the function of the TolA protein

Molecular Microbiology

Volumn 38, Issue 2, 2000, Pages 262-275

  Gaspar, Joe A ^a   Thomas, John A ^a   Marolda, Cristina L ^a   Valvano, Miguel A ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; MANNOSE; MUTANT PROTEIN; O ANTIGEN; PROTEIN TOLA; PROTEIN TOLB; PROTEIN TOLQ; PROTEIN TOLR; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; ESCHERICHIA COLI; FRAMESHIFT MUTATION; LETHALITY; MOLECULAR CLONING; NONHUMAN; OPERON; PHENOTYPE; PLASMID; PRIORITY JOURNAL;

BACTERIAL PROTEINS; CELL MEMBRANE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; ISOTOPE LABELING; MANNOSE; MEMBRANE PROTEINS; MULTIGENE FAMILY; MUTAGENESIS; O ANTIGENS; PERIPLASMIC PROTEINS; TRANSCRIPTION, GENETIC;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12; GOSSWEILERODENDRON BALSAMIFERUM; NEGIBACTERIA; PROKARYOTA;

EID: 0033744772     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.02094.x     Document Type: Article

References (74)

1. Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine
2. Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: Insertion mutagenesis of the rfa locus
3. Uptake across the cell envelope and insertion into the inner membrane of ion channel-forming colicins in E. coli
4. The fusion sites between outer membrane and cytoplasmic membrane of bacteria: Their roles in membrane assembly and virus infection
5. Zones of membrane adhesion in the cryofixed envelope of Escherichia coli
6. Enzymes 1: Oxidoreductases, transferases
7. Escherichia coli tol-pal mutants form outer membrane vesicles
8. Peptidoglycan-associated lipoprotein-TolB interaction. A possible key to explaining the formation of contact sites between the inner and outer membranes of Escherichia coli
9. Escherichia coli tolQ mutants are resistant to filamentous bacteriophages that adsorb to the tips, not the shafts, of conjugative pili
10. The structurally related exbB and tolQ genes are interchangeable in conferring tonB-dependent colicin, bacteriophage, and albomycin sensitivity
11. Identification of an ATP-binding cassette transport system required for translocation of lipopolysaccharide O-antigen side-chains across the cytoplasmic membrane of Klebsiella pneumoniae serotype O1
12. Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product
13. TolB protein of Escherichia coli K-12 interacts with the outer membrane peptidoglycan-associated proteins Pal, Lpp and OmpA
14. Identification and characterization of the tolQRA genes of Pseudomonas aeruginosa
15. Protein complex within Escherichia coli inner membrane. TolA N-terminal domain interacts with TolQ and TolR proteins
16. Tol A central domain interacts with Escherichia coli porins
17. The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat
18. Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12
19. Colicin A and the Tol proteins involved in its translocation are preferentially located in the contact sites between the inner and outer membranes of Escherichia coli cells
20. CTXphi infection of Vibrio cholerae requires the tolQRA gene products
21. Isolation of differentiated membrane domains from Escherichia coli and Salmonella typhimurium, including a fraction containing attachment sites between the inner and outer membranes and the murein skeleton of the cell envelope
22. Maturation and localization of the TolB protein required for colicin import
23. Complement evasion by bacteria and parasites
24. Outer membrane protein profiles and multilocus enzyme electrophoresis analysis for differentiation of clinical isolates of Proteus mirabilis and Proteus vulgaris
25. Genetics and biosynthesis of lipopolysaccharide O antigens
26. Further electron microscopic studies on the expression of Escherichia coli group II capsules
27. Colicin import into Escherichia coli Cells
28. Transmembrane alpha-helix interactions are required for the functional assembly of the Escherichia coli Tol complex
29. The Tol proteins of Escherichia coli and their involvement in the uptake of biomolecules and outer membrane stability
30. TonB protein appears to transduce energy by shuttling between the cytoplasmic membrane and the outer membrane in Escherichia coli
31. Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli
32. TolA: A membrane protein involved in colicin uptake contains an extended helical region
33. Role of the carboxyl-terminal domain of TolA in protein import and integrity of the outer membrane
34. Escherichia coli K12 regains its O antigen
35. An O-antigen processing function for Wzx (RfbX): A promising candidate for O-unit flippase
36. Structural studies of the O-specific side chain of the lipopolysaccharide from Escherichia coli O:7
37. Localization of the terminal steps of O-antigen synthesis in Salmonella typhimurium
38. Evidence for energy-dependent transposition of core lipopolysaccharide across the inner membrane of Salmonella typhimurium
39. Energy dependence of lipopolysaccharide translocation in Salmonella typhimurium
40. Energy dependence of O-antigen synthesis in Salmonella typhimurium
41. The promoter region of the Escherichia coli O7-specific lipopolysaccharide gene cluster: Structural and functional characterization of an upstream untranslated mRNA sequence
42. Identification, expression, and DNA sequence of the GDP-mannose biosynthesis genes encoded by the O7 rfb gene cluster of strain VW187 (Escherichia coli O7:K1)
43. Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: Identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene
44. Genetic analysis of the O7-polysaccharide biosynthesis region from the Escherichia coli O7:K1 strain VW187
45. Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1)
46. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers
47. Impairment of cell division in tolA mutants of Escherichia coli at low and high medium oslomarities
48. A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
49. An intermediate step in translocation of lipopolysaccharide to the outer membrane of Salmonella typhimurium
50. Genetics and physiology of colicin-tolerant mutants of Escherichia coli
51. Altered deoxyribonucleic pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene
52. Molecular basis of bacterial outer membrane permeability
53. Mutants d'Escherichia coli K-12 affectes pour leur croissance sur methyl-B-D-glucuronide: Localisation du gene de structure de la B-glucuronidase (uid)
54. Studies in the Gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonoate in the lipopolysaccharide of Salmonella typhimurium
55. In vitro insertional mutagenesis with a selectable DNA fragment
56. Bacterial lipopolysaccharides: A remarkable family of bioactive molecule
57. An nptl-sacB-sacR cartridge for constructing directed, unmarked mutations in Gram-negative bacteria by marker exchange-eviction mutagenesis
58. The TolB protein interacts with the porins of Escherichia coli
59. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
60. Genetics of lipopolysaccharide biosynthesis in enteric bacteria
61. fii, a bacterial locus required for filamentous phage infection and its relation to colicin-tolerant tolA and tolB
62. Nucleotide sequence of a gene cluster involved in the entry of E colicins and single stranded DNA of infecting filamentous bacteriophages into Escherichia coli
63. Evaluation of a techniques for the estimation of serum bactericidal activity against gram-negative organisms
64. tolQ is required for cloacin DF13 susceptibility in Escherichia coli expressing the aerobactin/cloacin DF13 receptor lutA
65. Role of tol genes in cloacin DF13 susceptibility of Escherichia coli K-12 strains expressing the cloacin DF13-aerobactin receptor lutA
66. Aerobactin iron transport genes commonly encoded by certain ColV plasmids occur in the chromosome of a human invasive strain of Escherichia coli K1
67. Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O7 lipopolysaccharide antigen of a human invasive strain of E. coli O7:K1
68. Relatedness of O-specific lipopolysaccharide side chain genes from strains of Shigella boydii type 12 belonging to two clonal groups and from Escherichia coli O7:K1
69. Characterization of the tol-pal region of Escherichia coli K-12: Translational control of tolR expression by TolQ and identification of a new open reading frame downstream of pal encoding a periplasmic protein
70. The tol gene products and the import of macromolecules into Escherichia coli
71. Biosynthesis of lipopolysaccharide O antigens
72. Structure, assembly and regulation of expression of capsules in Escherichia coli
73. Biosynthesis and expression of cell-surface polysaccharides in gram-negative bacteria