Digitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA

Brain Research Protocols

Volumn 6, Issue 1-2, 2000, Pages 86-90

  Tramontina, Francine ^a   Karl, Juliana ^a   Gottfried, Carmem ^b   Mendez, Andreas ^a   Gonçalves, Daniela ^a   Portela, Luis V ^a   Gonçalves, Carlos Alberto ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b PONTIFICAL CATHOLIC UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Astrocyte; Cell permeabilization; Digitonin; ELISA; S100B; Trypan blue

Indexed keywords

CALCIUM ION; DIGITONIN; DIVALENT CATION; NUCLEOTIDE; PHOSPHATIDYLINOSITIDE; PROTEIN S 100; TRYPAN BLUE;

ANIMAL CELL; ARTICLE; ASTROCYTE; ENZYME LINKED IMMUNOSORBENT ASSAY; NERVE CELL CULTURE; NONHUMAN; PERMEABILITY; PRIORITY JOURNAL; RAT;

ADENOSINE TRIPHOSPHATE; ANIMALS; ASTROCYTES; CALCIUM; CALCIUM-BINDING PROTEINS; CATTLE; CELL MEMBRANE PERMEABILITY; CELLS, CULTURED; CHELATING AGENTS; COLORING AGENTS; DIGITONIN; EGTAZIC ACID; ENZYME-LINKED IMMUNOSORBENT ASSAY; NERVE GROWTH FACTORS; RATS; S100 PROTEINS; TRYPAN BLUE;

EID: 0033742127     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(00)00041-6     Document Type: Article

References (15)

1. Regulation of protein phosphorylation in astrocyte cultures by external calcium ions: Specific effects on the phosphorylation of glial fibrillary acidic protein (GFAP), vimentin and heat shock protein 27 (HSP27)
2. 2+ buffers
3. GFAP phosphorylation studied in digitonin-permeabilized astrocytes: Standardization of conditions
4. A specific and sensitive ELISA for measuring S-100B in cerebrospinal fluid
5. Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromaffin cells
6. 2+ -evoked catecholamine release
7. The use of permeabilized cell to assay protein phosphorylation and catecholamine release
8. Permeabilizing cells: Some methods and applications for the study of intracellular process
9. Calcium homeostasis in digitonin-permeabilized bovine chromaffin cells
10. Regulated exocytosis secretion from permeabilized cells
11. Astrocytes possess prostaglandin F(2α) receptors coupled to phospholipase C
12. Cell resealing by a vesicular mechanism similar to neurotransmitter release
13. Impaired Ca-signaling in astrocytes from Ts16 mouse model of Down syndrome
14. 2+: A study of antibody recognition in a dot immunoassay for calmodulin, calcineurin and S100B
15. Simultaneous measurement of tyrosine hydroxylase activity and phosphorylation in bovine adrenal chromaffin cells