Simultaneous measurement of tyrosine hydroxylase activity and phosphorylation in bovine adrenal chromaffin cells

Journal of Neuroscience Methods

Volumn 87, Issue 2, 1999, Pages 167-174

  Cheah, Tat Beng ^a   Bobrovskaya, Larisa ^a   Gonçalves, Carlos Alberto ^c   Hall, Amanda ^a   Elliot, Robert ^a   Lengyel, Imre ^a   Bunn, Stephen J ^d   Marley, Philip D ^b   Dunkley, Peter R ^a  

^a UNIVERSITY OF NEWCASTLE   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^d UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Catecholamines; Chromaffin cells; Digitonin permeabilisation; Protein phosphorylation; Tyrosine hydroxylase activity

Indexed keywords

ADENOSINE TRIPHOSPHATE; CARBON 14; CARBON DIOXIDE; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; FORSKOLIN; N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE; PHOSPHATE; SERINE; SODIUM HYDROXIDE; TYROSINE 3 MONOOXYGENASE; TYROSINE DERIVATIVE;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; AUTORADIOGRAPHY; CATTLE; CHROMAFFIN CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ADRENAL MEDULLA; ANIMALS; BIOLOGICAL ASSAY; CATECHOLAMINES; CATTLE; CELLS, CULTURED; CHROMAFFIN CELLS; CYCLIC AMP; DIGITONIN; ENZYME INHIBITORS; FORSKOLIN; INDICATORS AND REAGENTS; ISOQUINOLINES; PERMEABILITY; PHOSPHORYLATION; SOLUBILITY; SULFONAMIDES; TYROSINE 3-MONOOXYGENASE;

EID: 0033105015     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00002-3     Document Type: Article

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