Conformational heterogeneity of P-glycoprotein

Cancer Detection and Prevention

Volumn 24, Issue 5, 2000, Pages 415-421

  Goda, K ^a   Nagy, H ^a   Bene, L ^a   Balazs, M ^a   Arceci, R ^a   Mechetner, E ^a   Szabo, G ^a  

^a UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Fluorescence resonance energy transfer; Multidrug resistance; Topology

Indexed keywords

EPITOPE; FIXATIVE; GLYCOPROTEIN P; METHANOL; MONOCLONAL ANTIBODY;

ANIMAL; ARTICLE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL STRAIN 3T3; CELL STRAIN KB; CHEMISTRY; CONFOCAL MICROSCOPY; DRUG EFFECT; ENERGY TRANSFER; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; IMMUNOLOGY; METABOLISM; MOUSE; PROTEIN CONFORMATION;

3T3 CELLS; ANIMALS; ANTIBODIES, MONOCLONAL; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; ENERGY TRANSFER; EPITOPES; FIXATIVES; FLOW CYTOMETRY; FLUORESCENCE; HUMANS; KB CELLS; METHANOL; MICE; MICROSCOPY, CONFOCAL; P-GLYCOPROTEIN; PROTEIN CONFORMATION;

EID: 0033670124     PISSN: 0361090X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

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4. Genetic analysis of the multidrug transporter
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6. Functionally active homodimer of P-glycoprotein in multidrug-resistant tumor cells
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8. Binding properties of monoclonal antibodies recognizing external epitopes of the human mdr1 P-glycoprotein
9. Efficient inhibition of P-glycoprotein-mediated multidrug resistance with monoclonal antibody
10. P-glycoprotein function involves conformational transitions detectable by differential immunoreactivity
11. Modulation of human P-glycoprotein epitope expression by temperature and/or resistance modulating agents
12. Human P-glycoprotein exhibits reduced affinity for substrates during a catalytic transition state
13. Experimental methods to measure fluorescence resonance energy transfer
14. Flow cytometric measurement of fluorescence resonance energy transfer on cell surfaces: Quantitative evaluation of the transfer efficiency on a cell-by-cell basis
15. Fluorescence energy transfer as a spectroscopic ruler
16. Multiple drug-resistant human KB carcinoma cells independently selected for high level resistance to colchicine, Adriamycin or vinblastine show changes in expression of specific proteins
17. Characterization of the azidopine and vinblastine binding site of P-glycoprotein
18. Functional role of the 170- 180-kd glycoprotein specific to drug-resistant tumor cells as revealed by monoclonal antibodies
19. Monoclonal antibody to an external epitope of the human mdr1 P-glycoprotein
20. Hydrophobic adsorption chromatography to reduce nonspecific staining by rhodamine-labeled antibodies
21. P-glycoprotein drug efflux pump involved in the mechanisms of intrinsic drug resistance in various colon cancer cell lines. Evidence for a saturation of active daunorubicin transport
22. Proximity measurements of cell surface proteins by fluorescence resonance energy transfer
23. Nuclear immunolocalization of P-glycoprotein in multidrug-resistant cell lines showing similar mechanisms of doxorubicin distribution
24. P-glycoprotein expression in the Golgi apparatus of multidrug-resistant cells
25. Functional intracellular P-glycoprotein
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28. Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance
29. Major histocompatibility complex class I protein conformation altered by transmembrane potential changes