Inhibition of IκB-α phosphorylation at serine and tyrosine acts independently on sensitization to DNA damaging agents in human glioma cells

British Journal of Cancer

Volumn 82, Issue 1, 2000, Pages 28-33

  Miyakoshi, J ^a   Yagi, K ^b  

^a Kyoto Umversity Graduate School of Medicine   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

DNA damaging agents; Human glioma cells; I B ; NF B; Phosphorylation

Indexed keywords

DOXORUBICIN; HERBIMYCIN A; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INHIBITOR PROTEIN; PHOSPHOSERINE; PHOSPHOTYROSINE; SERINE; TYROSINE; ANTINEOPLASTIC AGENT; BENZOQUINONE DERIVATIVE; CHUK PROTEIN, HUMAN; DNA; ENZYME INHIBITOR; GUANOSINE 3',5' POLYPHOSPHATE SYNTHETASES; GUANOSINE 3',5'-POLYPHOSPHATE SYNTHETASES; HERBIMYCIN; I KAPPA B KINASE; IKBKB PROTEIN, HUMAN; IKBKE PROTEIN, HUMAN; LIGASE; MACROCYCLIC LACTAM; PROTEIN SERINE THREONINE KINASE; QUINONE DERIVATIVE; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CONTROLLED STUDY; DNA DAMAGE; DRUG SENSITIVITY; GLIOMA CELL; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RADIOSENSITIVITY; ULTRAVIOLET RADIATION; X RAY; CELL CULTURE; CELL CYCLE; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ACTIVATION; ENZYMOLOGY; GENE DELETION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; GLIOMA; METABOLISM; PHOSPHORYLATION; RADIATION DOSE; RADIATION EXPOSURE; RADIATION RESPONSE; TIME;

ANTINEOPLASTIC AGENTS; BENZOQUINONES; CELL CYCLE; DNA DAMAGE; DNA, NEOPLASM; DOSE-RESPONSE RELATIONSHIP, RADIATION; DOXORUBICIN; ENZYME ACTIVATION; ENZYME INHIBITORS; GENETIC VECTORS; GLIOMA; HUMANS; I-KAPPA B KINASE; LACTAMS, MACROCYCLIC; LIGASES; NF-KAPPA B; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; QUINONES; RADIATION TOLERANCE; SEQUENCE DELETION; SERINE; TIME FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR-ALPHA; TYROSINE; ULTRAVIOLET RAYS;

EID: 0033621903     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1054/bjoc.1999.0872     Document Type: Article

References (31)

1. Alkalay I, Yaron A, Hatzubai A, Orian A, Ciechanover A and Ben-Neriah Y (1995) Stimulation-dependent IκBα phosphorylation marks the NF-κB inhibitor for degradation via the ubiquitin-proteasome pathway. Proc Natl Acad Sci USA 92: 10599-10603
2. Baeuerle PA (1991) The inducible transcription activator NF-κB: regulation by distinct protein subunits. Biochim Biophys Acta 1072: 63-80
3. Baeuerle PA and Baltimore D (1988) IκB-α: a specific inhibitor of the NF-κB transcription factor. Science 242: 540-546
4. Baeuerle PA and Henkel T (1994) Function and activation of NF-κB in immune system. Annu Rev Immunol 12: 141-179
5. Beg AA, Rubin SM, Scheinman RI, Haskill S, Rosen CA and Baldwin AS (1992) IκB interacts with the nuclear localization sequencers of the subunits of NF-κB: a mechanism for cytoplasmic retention. Genes Dev 6: 1899-1913
6. Beg A A, Finc TS, Nantermet PV and Baldwin AS Jr (1993) Tumor necrosis factor and interleukin-1 lead to phosphorylation and loss of IκB-α: a mechanism for NF-κB activation. Mol Cell Biol 13: 13301-13310
7. Brockman JA, Scherer DC, McKinsey TA, Hall SM. Qi X, Young Lee W and Ballard DW (1995) Coupling of a signal response domain in IκBα to multiple pathways for NF-κB activation. Mol Cell Biol 15: 2809-2818
8. Brown K, Park S, Kanno T, Franzoso G and Siebenlist U (1993) Mutual regulation of the transcriptional activator NF-κB and its inhibitor, I kappa B-α. Proc Natl Acad Sci USA 90: 2532-2536
9. Brown K, Gerstberger S, Carlson L, Franzoso G and Siebenlist U (1995) Control of IκB-α proteolysis by site-specific, signal-induced phosphorylation. Science 267: 1485-1488
10. Cordle SR, Donald R, Read MA and Hawiger J (1993) Lipopolysaccharide induces phosphorylation of MAD3 and activation of c-Rel and related NF-κB proteins in human monocytic THP-1 cells. J Biol Chem 268: 11803-11810
11. Hallahan DE, Sukhatme VP, Sherman ML, Virudaghalam S, Kufe D and Weichselbaum RR (1991) Protein kinase C mediates x-ray inducibility of nuclear signal transducers EGRI and JUN. Proc Natl Acad Sci USA 88: 2156-2160
12. Imbert V, Rupec RA, Livolsi A, Pah HL, Traenckner EB-M, Mueller-Dicckman C, Farahifer D, Rossi B, Auberger P, Baeuerle PA and Peyron J-F (1996) Tyrosine phosphorylation of IκB-α activates NF-κB without proteolytic degradation of IκB-α. Cell 86: 787-798
13. Iwasaki T, Uehara Y, Gravea L, Rachie N and Bomsztyk K (1992) Herbimycin A blocks IL-1-induced NF-κB DNA-binding activity in lymphoid cell lines. FEBS Lett 298: 240-244
14. Jung M, Zhang Y, Lee S and Dritschilo A (1995) Correction of radiation sensitivity in Ataxia Telangiectasia cells by a truncated IκB-α. Science 268: 1619-1621
15. Kasid U, Pfeifer A, Brenman T, Beckett M, Weichselbaum RR, Drilschilo A and Mark GE (1989) Effect of antisense c-raf-l on tumorigenicity and radiation sensitivity of a human squamous carcinoma. Science 243: 1354-1356
16. Mellits KH, Hay RT and Goodbourn S (1993) Proteolytic degradation of MAD3 (1κB alpha) and enhanced processing of the NF-κB precursor p105 are obligatory steps in the activation of NF-κB. Nucelic Acids Res 21: 5059-5066
17. Miyakoshi J, Yamagishi N, Ohtsu S and Takebe H (1995) Changes in radiation sensitivity of human osteosarcoma cells after p53 introduction. Jap J Cancer Res 86: 711-713
18. Miyakoshi J, Yamagishi N, Ohtsu S, Mohri K and Takebe H (1996) Exposure to magnetic field (5 mT at 60 Hz) does not affect cell growth and c-myc gene expression. Mutat Res 349: 109-114
19. Miyakoshi J, Kitagawa K, Yamagishi N, Ohtsu S, Day RS III and Takebe H (1997) Increased radiosensitivity of p16 gene-deleted human glioma cells after transfection with wild-type p16 gene. Jap J Cancer Res 88: 34-38
20. Morhan N and Meltz ML (1994) Induction of nuclear factor κB after low-dose ionizing radiation involves a reactive oxygen intermediate signaling pathway. Radiat Res 140: 97-104
21. Pirollo KF, Tong YA, Villegas Z, Chen Y and Chang EH ( 1993) Oncogene-transformed NIH 3T3 cells display radiation resistance levels indicative of a signal transduclion pathway leading to the radiation-resistant phenotype. Radiat Res 135: 234-243
22. Scherer DC, Brockman JA, Chen Z, Maniatis T and Ballard DW (1995) Signal-induced degradation of IκBα requires site-specific ubiquitination. Proc Natl Acad Sci USA 92: 11259-11263
23. Schreiber E, Matthias P, Mulle MM and Schaffner W (1989) Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells. Nucleic Acids Res 17: 6419
24. Sklar MD (1988) The ras oncogenes increase the intrinsic resistance of HIH 3T3 cells to ionizing radiation. Science 239: 645-647
25. Traenckner EB-M, Pahl HL, Henke T, Schmidt KN, Wilk S and Baererle PA (1995) Phosphorylation of human IκB-α on serines 32 and 36 control IκB-α proteolysis and NF-κB activation in response to diverse stimuli. EMBO J 14: 2876-2883
26. Uckun FM, Schieven GL, Tuel-Ahlgren LM, Dibirdik I, Myers DE, Ledbetter JA and Song CW (1993) Tyrosine phosphorylation is a mandatory proximal step in radiation-induced activation of the protein kinase C signaling pathway in human B-lymphocyte precursors. Proc Natl Acad Sci USA 90: 252-256
27. Yamagishi N, Miyakoshi J and Takebe H (1997a) Decrease in the frequency of x-ray-induced mutation by wild-type p53 protein in human osteosarcoma cells. Carcinogenesis 18: 695-700
28. Yamagishi N, Miyakoshi J, Yagi T and Takebe H (1997b) Suppression of UV-induced mutation by wild-type p53 protein in human osteosarcoma cells. Mutagenesis 12: 191-194
29. Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D and Miyamoto S (1995) Rel/NF-κB family: intimate tales of association and dissociation. Genes Dev 9: 2723-2735
30. Wang C-Y, Mayo MW and Baldwin AS Jr (1996) TNF-and cancer therapy-induced apoptosis: potentiation by inhibition of NF-κB. Science 274: 784-787
31. Wilson RE, Taylor SL, Atherton GT, Johnston D, Waters CM and Norton JD (1993) Early response gene signalling cascades activated by ionizing radiation in primary human B cells. Oncogene 8: 3229-3237