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Volumn 283, Issue 5402, 1999, Pages 693-695

Immortal time: Circadian clock properties of rat suprachiasmatic cell lines

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL MARKER; DEOXYGLUCOSE; NEUROTROPHIN;

EID: 0033613828     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.283.5402.693     Document Type: Article
Times cited : (113)

References (21)
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    • Z. S. Sun et al., ibid. 90, 1003 (1997).
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    • H. Tei et al., Nature 389, 512 (1998).
    • (1998) Nature , vol.389 , pp. 512
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    • D. C. Klein, R. Y. Moore, S. M. Reppert, Eds. Oxford Univ. Press, New York
    • W. J. Schwartz, in Suprachiasmatic Nucleus: The Mind's Clock, D. C. Klein, R. Y. Moore, S. M. Reppert, Eds. (Oxford Univ. Press, New York, 1991), pp. 144-156.
    • (1991) Suprachiasmatic Nucleus: The Mind's Clock , pp. 144-156
    • Schwartz, W.J.1
  • 11
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    • 14C-labeled 2-DC (0.2 mCi/ml; American Radiological Company, St. Louis, MO). Fractional products of 2-DC metabolism were measured with the methods described by Newman and colleagues [G. C. Newman, F. E. Hospod, C. S. Patlak, J. Cereb. Blood Flow Metab. 10, 510 (1990)]. Fractions derived from 2-DC, 2-DG-6P, free 2-DG, and glycogen compartments were placed in scintillation vials in triplicate, dried before addition of scintillant, and then counted on a Beckman scintillation counter.
    • (1990) J. Cereb. Blood Flow Metab. , vol.10 , pp. 510
    • Newman, G.C.1    Hospod, F.E.2    Patlak, C.S.3
  • 12
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    • note
    • 14C-labeled 2-DG uptake and neurotrophin content were normalized for sample protein content as measured by the bicinchoninic acid method (Pierce). This analysis was replicated on three separate sets of cultures. Time-dependent alterations in glucose utilization and neurotrophin levels were identified with one-way analysis of variance, and differences between determinations at distinct time points were tested post hoc for significance with the Newman-Keuls sequential range test.
  • 15
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    • note
    • 2 periodogram (Tau) and fast Fourier transform analyses. After behavioral analysis, animals were killed with sodium pentobartital (3.0 mg/kg) and perfused transcardially with 4% paraformaldehyde in 0.1 M phosphate buffer. Animal care and procedures were performed in compliance with state law, federal statute, and NIH policy.
  • 16
    • 0345320244 scopus 로고    scopus 로고
    • note
    • -5 M), except for occasional light staining in the area of gliosis surrounding the lesion site.
  • 21
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    • note
    • We thank D. Bell-Pedersen for comments on the manuscript. This study was supported by NSF grant IBN-9511238 (D.J.E.) and NIH grant NS35822 (V.M.C).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.