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Volumn 121, Issue 29, 1999, Pages 6850-6855

Lead fingers: Pb2+ binding to structural zinc-binding domains determined directly by monitoring lead-thiolate charge-transfer bands

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; LEAD; METAL COMPLEX; ZINC;

EID: 0033612767     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja990899o     Document Type: Article
Times cited : (132)

References (48)
  • 8
    • 85069247661 scopus 로고    scopus 로고
    • note
    • 2+ to catalyze hydrolytic cleavage of RNA. (See refs 9 and 10.) However, studies on lead-catalyzed cleavage of RNA in vitro indicate that micromolar to millimolar concentrations of free lead are required for efficient cleavage. (See refs 9-14.) Such high concentrations of lead are unlikely to be physiologically relevant.
  • 17
    • 85069257217 scopus 로고    scopus 로고
    • The sequence for the zinc finger consensus peptide that has four cysteines in the zinc-binding site (CP-CCCC) is the following: PYKCPECGKSFSQKSDLVKCQRTCTG
    • The sequence for the zinc finger consensus peptide that has four cysteines in the zinc-binding site (CP-CCCC) is the following: PYKCPECGKSFSQKSDLVKCQRTCTG.
  • 18
    • 85069246849 scopus 로고    scopus 로고
    • The sequence for the zinc finger consensus peptide that has three cysteines and one histidine in the zinc-binding site (CP-CCHC) is the following: PYKCPECGKSFSQKSDLVKHQRTCTG
    • The sequence for the zinc finger consensus peptide that has three cysteines and one histidine in the zinc-binding site (CP-CCHC) is the following: PYKCPECGKSFSQKSDLVKHQRTCTG.
  • 19
    • 85069245584 scopus 로고    scopus 로고
    • The sequence for the zinc finger consensus peptide that has two cysteines and two histidines in the zinc-binding site (CP-CCHH) is the following: PYKCPECGKSFSQKSDLVKHQRTHTG
    • The sequence for the zinc finger consensus peptide that has two cysteines and two histidines in the zinc-binding site (CP-CCHH) is the following: PYKCPECGKSFSQKSDLVKHQRTHTG.
  • 22
    • 85069256699 scopus 로고    scopus 로고
    • The sequence for the zinc-binding domain from the HIV-1 nucleocapsid protein (HIV-CCHC) is the following: VKCFNCGKEGHIARNCRA
    • The sequence for the zinc-binding domain from the HIV-1 nucleocapsid protein (HIV-CCHC) is the following: VKCFNCGKEGHIARNCRA.
  • 23
    • 85069257182 scopus 로고    scopus 로고
    • Synthetic peptides were purchased from either Biosynthesis (P.O. Box 28, Lewisville, TX 75067-0028) or the Macromolecular Structure Facility at Michigan State University (Department of Biochemistry, East Lansing, MI 48824)
    • Synthetic peptides were purchased from either Biosynthesis (P.O. Box 28, Lewisville, TX 75067-0028) or the Macromolecular Structure Facility at Michigan State University (Department of Biochemistry, East Lansing, MI 48824).
  • 24
    • 85069253828 scopus 로고    scopus 로고
    • The following molecular weights were obtained by mass spectrometry: CP-CCHH 2962.4 (calculated 2962.4); CP-CCHC 2928.4 (calculated 2928.4); CP-CCCC 2894.3 (calculated 2894.4); HIV-CCHC 2014.6 (calculated 2006.4). Mass spectra were obtained with the peptides from Biosynthesis
    • The following molecular weights were obtained by mass spectrometry: CP-CCHH 2962.4 (calculated 2962.4); CP-CCHC 2928.4 (calculated 2928.4); CP-CCCC 2894.3 (calculated 2894.4); HIV-CCHC 2014.6 (calculated 2006.4). Mass spectra were obtained with the peptides from Biosynthesis.
  • 25
    • 85069240521 scopus 로고    scopus 로고
    • Molar extinction coefficients for CP-CCHH, CP-CCHC, and CP-CCCC were obtained from J. M. Berg and co-workers (personal communication)
    • Molar extinction coefficients for CP-CCHH, CP-CCHC, and CP-CCCC were obtained from J. M. Berg and co-workers (personal communication).
  • 26
    • 85069259814 scopus 로고    scopus 로고
    • note
    • The molar extinction coefficient for HIV-CCHC was calculated from the absorbance spectrum of a peptide stock solution that had been quantitated by amino acid analysis. Amino acid analysis was conducted by the Keck Biophysics Facility at Yale University.
  • 28
    • 85069238746 scopus 로고    scopus 로고
    • SPECFIT is a product of Spectrum Software Associates and is owned solely by the authors, Robert Binstead and Andreas Zuberbuhler
    • SPECFIT is a product of Spectrum Software Associates and is owned solely by the authors, Robert Binstead and Andreas Zuberbuhler.
  • 36
    • 85069259007 scopus 로고    scopus 로고
    • NOESY spectrum of Pb(HIV-CCHC) at 4°C (not shown) was obtained at the Biological Sciences Division Nuclear Magnetic Resonance Facility at the University of Chicago
    • NOESY spectrum of Pb(HIV-CCHC) at 4°C (not shown) was obtained at the Biological Sciences Division Nuclear Magnetic Resonance Facility at the University of Chicago.
  • 47
    • 0011797091 scopus 로고
    • Historical Records of Metal Pollution in the Environment
    • Nriagu, J. O., Ed.; Springer-Verlag: New York
    • Thorton, I.; Abrahams, P. Historical Records of Metal Pollution in the Environment. In Changing Metal Cycles and Human Health; Nriagu, J. O., Ed.; Springer-Verlag: New York, 1984; pp 7-25.
    • (1984) Changing Metal Cycles and Human Health , pp. 7-25
    • Thorton, I.1    Abrahams, P.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.