Directed evolution to investigate steric control of enzymatic oxidosqualene cyclization. An isoleucine-to-valine mutation in cycloartenol synthase allows lanosterol and parkeol biosynthesis [9]

Journal of the American Chemical Society

Volumn 121, Issue 42, 1999, Pages 9887-9888

  Hart, Elizabeth A ^a   Hua, Ling ^a   Darr, Lisa B ^a   Wilson, William K ^a   Pang, Jihai ^a   Matsuda, Seiichi P T ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOARTENOL; ISOLEUCINE; LANOSTEROL; SQUALENE DERIVATIVE; SYNTHETASE; VALINE;

AMINO ACID SUBSTITUTION; BIOSYNTHESIS; CATALYSIS; CYCLIZATION; ENZYME ACTIVITY; FUNGUS MUTANT; LETTER; NONHUMAN; YEAST;

EID: 0033610475     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja992589b     Document Type: Letter

References (27)

1. Matsuda, S. P. T. On the Diversity of Oxidosqualene Cyclases. In Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers; Steinbüchel, A., Ed.; Wiley-VCH: Weinheim, 1998; pp 300-307.
2. Abe, I.; Rohmer, M.; Prestwich, G. D. Chem. Rev. 1993, 93, 2189-2206.
3. Corey, E. J.; Cheng, H. M.; Baker, C. H.; Matsuda, S. P. T.; Li, D.; Song, X. L. J. Am. Chem. Soc. 1997, 119, 1289-1296.
4. Kushiro, T.; Shibuya, M.; Ebizuka, Y. J. Am. Chem. Soc. 1999, 121, 1208-1216.
5. Kannenberg, E. L.; Poralla, K. Naturwissenschaften 1999, 86, 168-176.
6. Ochs, D.; Kaletta, C.; Entian, K.-D., Beck-Sickinger, A.; Poralla, K. J. Bacteriol. 1992, 174, 298-302.
7. Wendt, K. U.; Poralla, K.; Schulz, G. E. Science 1997, 277, 1811-1815.
8. Wendt, K. U.; Lenhart, A.; Schulz, G. E. J. Mol. Biol. 1999, 286, 175-187.
9. Merkofer, T.; Pale-Grosdemange, C.; Wendt, K. U.; Rohmër, M.; Poralla, K. Tetrahedron Lett. 1999, 40, 2121-2124.
10. Sato, T.; Abe, T.; Hoshino, T. Chem. Commun. 1998, 23, 2617-2618.
11. Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A. Y.; Cheng, H. Biochem. Biophys., Res. Commun. 1996, 219, 327-331.
12. Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628-11632.
13. Liu, H.; Krizek, J.; Bretscher, A. Genetics 1992, 132, 665-673.
14. Christianson, T. W.; Sikorski, R. S.; Dante, M.; Shero, J. H.; Hieter, P. Gene 1992, 110, 119-122.
15. Herrera, J. B. R.; Bartel, B., Wilson, W. K.; Matsuda, S. P. T. Phytochemistry 1998, 49, 1905-1911.
16. Nes, W. D.; Koike, K.; Jia, Z. H.; Sakamoto, Y.; Satou, T.; Nikaido, T.; Griffin, J. F. J. Am. Chem. Soc. 1998, 121, 5970-5980.
17. Emmons, G. T.; Wilson, W. K.; Schroepfer, G. J., Jr. Magn. Reson. Chem. 1989, 27, 1012-1024.
18. Pascal, R. A., Jr.; Farris, C. L.; Schroepfer, G. J., Jr. Anal. Biochem. 1980, 101, 15-22.
19. Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 97, 2211-2215.
20. Alani, E.; Cao, L.; Kleckner, N. Genetics 1987, 116, 541-545.
21. Boeke, J. D.; Trueheart, J.; Natsoulis, G.; Fink, G. R. Methods Enzymol. 1987, 154, 164-175.
22. Morita, M.; Shibuya, M.; Lee, M.-S.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. 1997, 20, 770-775.
23. Kushiro, T.; Shibuya, M.; Ebizuka, Y. Eur. J. Biochem. 1998, 256, 238-244.
24. Hayashi, H.; Hiraoka, N.; Ikeshiro, Y.; Kushiro, T.; Morita, M.; Shibuya, M.; Ebizuka, Y. GenBank 1999, AB025968.
25. Heintz, R.; Benveniste, P. J. Biol. Chem. 1974, 249, 4267-4274.
26. Hewlins, M. J. E.; Ehrhardt, J. D.; Hirth, L.; Ourisson, G. Eur. J. Biochem. 1969, 8, 184-188.
27. Schaefer, P. C.; De Reinach, F.; Ourisson, G. Eur. J. Biochem. 1970, 14, 284-288.