Biosynthesis of vitamin B6: Enzymatic conversion of 1-deoxy-D-xylulose- 5-phosphate to pyridoxol phosphate [14]

Journal of the American Chemical Society

Volumn 121, Issue 33, 1999, Pages 7722-7723

  Cane, David E ^a   Du, Shoucheng ^a   Robinson, J Kenneth ^a   Hsiung, Yuju ^a   Spenser, Ian D ^b  

^a BROWN UNIVERSITY   (United States)

^b MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PYRIDOXAL 5 PHOSPHATE; PYRIDOXINE; XYLULOSE;

AMINO ACID METABOLISM; BIOSYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; LETTER; NONHUMAN; PROTEIN PURIFICATION; PROTON NUCLEAR MAGNETIC RESONANCE;

EID: 0033603893     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9914947     Document Type: Letter

References (24)

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19. Interestingly, although use of PdxA as a query and either the BLAST or TFASTA algorithms to search the combined Genbank and EMBL databases turned up no significant matches to known proteins, use of the iterative PSI-BLAST algorithm (Altschul, S. F.; Madden, T. F.; Schäffer, A. A.; Zhang, J.; Zhang, Z.; Miller, W.; Lipman, D. J. Nucleic Acids Res. 1997, 25, 3389-3402; http://www.ncbi.nlm.nih.gov/cgi-bin/BLAST/nph-psi_blast) to search the NCBI nonredundant (nr) peptide sequence database revealed after 2 iterations ∼30-35% similarity over 150-200 amino acids both to isocitrate dehydrogenases and to 3-isopropylmalate dehydrogenases, enzymes that also catalyze nicotinamide-dependent decarboxylative oxidations of β-hydroxy acids.
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21. Sprenger, G. A.; Schörken, U.; Wiegert, T.; Grolle, S.; de Graaf, A. A.; Taylor, S. V.; Begley, T. P.; Bringer-Meyer, S.; Sahm, H. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 12857-12862. Lois, L. M.; Campos, N.; Rosa Putra, S.; Danielsen, K.; Rohmer, M.; Boronat, A. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 2105-2110. Glyceraldehyde-3-phosphate was generated in situ from fructose-1,6-diphosphate using aldolase and triose phosphate isomerase.
22. Sprenger, G. A.; Schörken, U.; Wiegert, T.; Grolle, S.; de Graaf, A. A.; Taylor, S. V.; Begley, T. P.; Bringer-Meyer, S.; Sahm, H. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 12857-12862. Lois, L. M.; Campos, N.; Rosa Putra, S.; Danielsen, K.; Rohmer, M.; Boronat, A. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 2105-2110. Glyceraldehyde-3-phosphate was generated in situ from fructose-1,6-diphosphate using aldolase and triose phosphate isomerase.
23. +, pyruvate, and LDH in the same proportions used in the assay, no absorption at 340 nm was detected.
24. After completion of this work, we were informed by Dr. Bernd Laber of Hoechst Schering AgrEvo Gmbh that he and his colleagues had also independently observed the formation of PNP from DXP catalyzed by PdxJ and that their results were in press (Laber, B.; Maurer, W.; Scharf, S.; Stepusin, K.; Schmidt, F. S. FEBS Lett. 1999, 449, 45-48.)