Homeotic transformation of rhombomere identity after localized Hoxb1 misexpression

Science

Volumn 284, Issue 5423, 1999, Pages 2168-2171

  Bell, Esther ^a   Wingate, Richard J T ^a   Lumsden, Andrew ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; BRAIN DEVELOPMENT; BRANCHIAL ARCH; CHICK EMBRYO; EMBRYO; EMBRYO DEVELOPMENT; NERVE PROJECTION; NEUROMERE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHOMBENCEPHALON;

ANIMALS; AXONS; BRANCHIAL REGION; CELL DIFFERENTIATION; CELL MOVEMENT; CHICK EMBRYO; CLONING, MOLECULAR; DNA-BINDING PROTEINS; GATA2 TRANSCRIPTION FACTOR; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, HOMEOBOX; GENETIC VECTORS; HOMEODOMAIN PROTEINS; MEMBRANE GLYCOPROTEINS; MOTOR NEURONS; RHOMBENCEPHALON; TRANSCRIPTION FACTORS;

ANIMALIA; VERTEBRATA;

EID: 0033603517     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5423.2168     Document Type: Article

References (35)

1. A. Lumsden and R. Krumlauf, Science 274, 1109 (1996).
2. A. Lumsden and R. Keynes, Nature 337, 424 (1989).
3. M. Cendron-Maguire, M. Mallo, M. Zhang, T. Gridley, Cell 75, 1317 (1993); F. M. Rijli et al., ibid., p. 1333.
4. M. Cendron-Maguire, M. Mallo, M. Zhang, T. Gridley, Cell 75, 1317 (1993); F. M. Rijli et al., ibid., p. 1333.
5. J. M. Coddard, M. Rossel, N. R. Manley, M. R. Capecchi, Development 122, 3217 (1996): M. Studer, A. Lumsden, L. Ariza-McNaughton, A. Bradley, R. Krumlauf, Nature 384, 630 (1996).
6. J. M. Coddard, M. Rossel, N. R. Manley, M. R. Capecchi, Development 122, 3217 (1996): M. Studer, A. Lumsden, L. Ariza-McNaughton, A. Bradley, R. Krumlauf, Nature 384, 630 (1996).
7. A. Cavalas, M. Davenne, A. Lumsden, P. Chambon, F. M. Rijli, Development 124, 3693 (1997): M. Mark et al., ibid. 119, 319 (1993); E. M. Carpenter, J. M. Goddard, O. Chisaka, N. R. Manley, M. R. Capecchi, ibid. 118, 1963 (1993); O. Chisaka, T. S. Musci, M. R. Capecchi, Nature 355, 516 (1992); A. Cavalas et al., Development 125, 1123 (1998); L Tiret, H. Le Mouellic, M. Maury, P. Brulet. ibid., p. 279.
8. A. Cavalas, M. Davenne, A. Lumsden, P. Chambon, F. M. Rijli, Development 124, 3693 (1997): M. Mark et al., ibid. 119, 319 (1993); E. M. Carpenter, J. M. Goddard, O. Chisaka, N. R. Manley, M. R. Capecchi, ibid. 118, 1963 (1993); O. Chisaka, T. S. Musci, M. R. Capecchi, Nature 355, 516 (1992); A. Cavalas et al., Development 125, 1123 (1998); L Tiret, H. Le Mouellic, M. Maury, P. Brulet. ibid., p. 279.
9. A. Cavalas, M. Davenne, A. Lumsden, P. Chambon, F. M. Rijli, Development 124, 3693 (1997): M. Mark et al., ibid. 119, 319 (1993); E. M. Carpenter, J. M. Goddard, O. Chisaka, N. R. Manley, M. R. Capecchi, ibid. 118, 1963 (1993); O. Chisaka, T. S. Musci, M. R. Capecchi, Nature 355, 516 (1992); A. Cavalas et al., Development 125, 1123 (1998); L Tiret, H. Le Mouellic, M. Maury, P. Brulet. ibid., p. 279.
10. A. Cavalas, M. Davenne, A. Lumsden, P. Chambon, F. M. Rijli, Development 124, 3693 (1997): M. Mark et al., ibid. 119, 319 (1993); E. M. Carpenter, J. M. Goddard, O. Chisaka, N. R. Manley, M. R. Capecchi, ibid. 118, 1963 (1993); O. Chisaka, T. S. Musci, M. R. Capecchi, Nature 355, 516 (1992); A. Cavalas et al., Development 125, 1123 (1998); L Tiret, H. Le Mouellic, M. Maury, P. Brulet. ibid., p. 279.
11. A. Cavalas, M. Davenne, A. Lumsden, P. Chambon, F. M. Rijli, Development 124, 3693 (1997): M. Mark et al., ibid. 119, 319 (1993); E. M. Carpenter, J. M. Goddard, O. Chisaka, N. R. Manley, M. R. Capecchi, ibid. 118, 1963 (1993); O. Chisaka, T. S. Musci, M. R. Capecchi, Nature 355, 516 (1992); A. Cavalas et al., Development 125, 1123 (1998); L Tiret, H. Le Mouellic, M. Maury, P. Brulet. ibid., p. 279.
12. A. Cavalas, M. Davenne, A. Lumsden, P. Chambon, F. M. Rijli, Development 124, 3693 (1997): M. Mark et al., ibid. 119, 319 (1993); E. M. Carpenter, J. M. Goddard, O. Chisaka, N. R. Manley, M. R. Capecchi, ibid. 118, 1963 (1993); O. Chisaka, T. S. Musci, M. R. Capecchi, Nature 355, 516 (1992); A. Cavalas et al., Development 125, 1123 (1998); L Tiret, H. Le Mouellic, M. Maury, P. Brulet. ibid., p. 279.
13. O. Alexandre et al., Development 122, 735 (1996); A. C. Burke and C. J. Tabin, Dev. Biol. 178, 192 (1996).
14. O. Alexandre et al., Development 122, 735 (1996); A. C. Burke and C. J. Tabin, Dev. Biol. 178, 192 (1996).
15. M. Zhang et al., Development 120, 2431 (1994).
16. H. Marshall et al., Nature 360, 737 (1992); J. Hill, J. D. Clarke, N. Vargesson, T. Jowett, N. Holder, Mech. Dev. 50, 3 (1995).
17. H. Marshall et al., Nature 360, 737 (1992); J. Hill, J. D. Clarke, N. Vargesson, T. Jowett, N. Holder, Mech. Dev. 50, 3 (1995).
18. P. Hunt et al., Nature 353, 361 (1991).
19. Whole-mount RNA in situ hybridizations were performed according to established protocols [D. Henrique et al., Nature 375, 787 (1995)]. All embryos stained for BEN and GATAZ were processed identically, Immunohistochemistry was performed with the BEN, QCPN (quail-specific marker; Hybridoma bank, IA), RMO-270 (recognizes the 155-kD neurofilament subunit; Zymed, CA), and antimouse Hoxb1 antibodies (M. Capecchi and N. Manley).
20. O. H. Sundin and G. A. Eichete, Genes Dev. 4, 1267 (1990).
21. 9 infectious viral particles per milliliter. Ectopic Hoxb1 protein was detected with a mouse-specific potyclonal antibody, and Hoxb1 mRNA by in situ hybridization, with a species-specific RNA probe.
22. 9 infectious viral particles per milliliter. Ectopic Hoxb1 protein was detected with a mouse-specific potyclonal antibody, and Hoxb1 mRNA by in situ hybridization, with a species-specific RNA probe.
23. 9 infectious viral particles per milliliter. Ectopic Hoxb1 protein was detected with a mouse-specific potyclonal antibody, and Hoxb1 mRNA by in situ hybridization, with a species-specific RNA probe.
24. V. Hamburger and L. Hamilton. J. Morphol. 88, 49 (1951).
25. O. Pourquié, M. Coltey, J.-L. Thomas, N. Le Douarin, Development 109, 743 (1990).
26. S. Guthrie and A. Lumsden, ibid. 114, 663 (1992).
27. I. Pata et al., in preparation.
28. E. Bell, A. Lumsden, A. Graham, Mech. Dev., in press.
29. H. Simon and A. Lumsden, Neuron 11, 209 (1993).
30. Retrograde labeling of motor neurons was performed by injection of either DII [Molecular Probes, OR; 6 mg/ml in dimethylformamide (DMF)] or DiO (Molecular Probes; 10 mg/ml in DMF) into the first or second branchial arch (or both). Embryos were stored in the dark at room temperature in 4% paraformaldehyde for 7 to 14 days. Hindbrains were dissected and examined by confocal microscopy (Bio-Rad MRC 600).
31. E. Bell, R. J. T. Wingate, A. Lumsden, data not shown.
32. R. J. T. Wingate and A. Lumsden, Development 122, 2143 (1996).
33. M. Goulding [Neuron 21, 943 (1998)] proposes a role for Hox genes in specifying the identity and pathfinding characteristics of motor neurons.
34. W. Bateson, Materials for the Study of Variation (Cambridge Univ. Press, Cambridge, 1894).
35. We are grateful to S. Hughes for the pCla12Nco, RCAS(BP)A, RCAN(BP)A, and RCAS(BP)B vectors; J. Gilthorpe for the RCAN(BP)B vector; and R. Krumlauf for the mouse Hoxbl cDNA, We thank V, Prince and A. Graham for the chick Hoxbl and GATAZ in situ probes, respectively, and M. Capecchi and N. Manley for the mouse antibody to Hoxb1. The BEN and QCPN monoclonal antibodies developed by N. Le Douarin and O. Pourquié and B. Carlson and J. Carlson, respectively, were obtained from the Developmental Studies Bank maintained by the University of Iowa, Department of Biological Sciences, under contracts N01-HD-7-3263 from the National Institute of Child Health and Human Development The Wellcome Trust and the Medical Research Council supported this work. R.J.T.W. is a recipient of a Wellcome Career Development Award.