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Volumn 284, Issue 5414, 1999, Pages 647-650

Nonproteolytic neuroprotection by human recombinant tissue plasminogen activator

Author keywords

[No Author keywords available]

Indexed keywords

ALTEPLASE; KAINIC ACID; ZINC;

EID: 0033597411     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5414.647     Document Type: Article
Times cited : (136)

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    • Male adult Sprague-Dawley rats (250 to 300 g) were used for experiments. The Animal Welfare Guidelines of University of Ulsan College of Medicine were followed. Under stereotactic guidance and halothane anesthesia, one group was injected into lateral ventricles with 3 μl of saline alone (icv saline group, n = 3), and another group with 3 μl of tPA (1 mg/ml) in saline (icv tPA group, n = 3). The final group received injection of tPA (3 mg/kg) into the tail veins (iv tPA group, n = 3). Anesthesia was discontinued immediately after the injections. After 30 min, rats were intraperitoneally injected with kainate (10 mg/kg). Three hours after the kainate injection, seizures were halted by intraperitoneally injecting sodium diphenylhydantoin (50 mg/kg).
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    • Supported by Creative Research Initiatives of the Korean Ministry of Science and Technology (J.Y.K.).


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