Heterologous expression of the vanadium-containing chloroperoxidase from Curvularia inaequalis in Saccharomyces cerevisiae and site-directed mutagenesis of the active site residues His496, Lys353, Arg360, and Arg490

Journal of Biological Chemistry

Volumn 274, Issue 34, 1999, Pages 23820-23827

  Hemrika, Wieger ^a   Renirie, Rokus ^a   Macedo Ribeiro, Sandra ^b   Messerschmidt, Albrecht ^b   Wever, Ron ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; CHLORIDE PEROXIDASE; HISTIDINE; LYSINE; PEROXIDASE; RECOMBINANT ENZYME; VANADIUM;

ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; GENE EXPRESSION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

CATALYTIC DOMAIN; CHLORIDE PEROXIDASE; KINETICS; MITOSPORIC FUNGI; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; STRUCTURE-ACTIVITY RELATIONSHIP; VANADIUM;

CURVULARIA INAEQUALIS; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0033588375     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.34.23820     Document Type: Article

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