Chemical treatment of Escherichia coli: 3. Selective extraction of a recombinant protein from cytoplasmic inclusion bodies in intact cells

Biotechnology and Bioengineering

Volumn 62, Issue 4, 1999, Pages 455-460

  Falconer, Robert J ^b   O'Neill, Brian K ^b   Middelberg, Anton P J ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b NONE

Author keywords

2 hydroxyethyldisulfide; Escherichia coli; Inclusion bodies; Insulin like growth factor

Indexed keywords

ACETIC ACID; AGENTS; CENTRIFUGATION; CHEMICAL BONDS; ESCHERICHIA COLI; EXTRACTION; INSULIN; OXIDATION; PROTEINS; PURIFICATION; SULFUR COMPOUNDS; UREA;

CHEMICAL PERMEABILIZATION;

CELL CULTURE;

RECOMBINANT PROTEIN;

ARTICLE; CELL INCLUSION; DISULFIDE BOND; ESCHERICHIA COLI; EXTRACTION; FILTRATION; NONHUMAN; PROTEIN ISOLATION; PROTEIN PURIFICATION;

BIOREACTORS; BIOTECHNOLOGY; CELL MEMBRANE PERMEABILITY; DISULFIDES; DITHIOTHREITOL; EDETIC ACID; ESCHERICHIA COLI; ETHANOL; INCLUSION BODIES; INDICATORS AND REAGENTS; INSULIN-LIKE GROWTH FACTOR I; RECOMBINANT PROTEINS; SOLUBILITY; UREA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033585859     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990220)62:4<455::AID-BIT8>3.0.CO;2-2     Document Type: Article

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