Chemical treatment of escherichia coli. II. Direct extraction of recombinant protein from cytoplasmic inclusion bodies in intact cells

Biotechnology and Bioengineering

Volumn 57, Issue 4, 1998, Pages 381-386

  Falconer, Robert J ^a   O'Neill, Brian K ^a   Middelberg, Anton P J ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Escherichia coli; IGF I; Inclusion bodies; Recombinant protein; Urea

Indexed keywords

CELL MEMBRANES; CELLS; COMPOSITION EFFECTS; DISSOLUTION; EXTRACTION; MECHANICAL PERMEABILITY; PH EFFECTS; PROTEINS; REACTION KINETICS; SULFUR COMPOUNDS; UREA; VISCOSITY;

CYTOPLASM; DITHIOTHREITOL; ETHYLENE DIAMINE TETRAACETIC ACID; RECOMBINANT PROTEIN;

COLIFORM BACTERIA;

BACTERIAL PROTEIN; EDETIC ACID; RECOMBINANT PROTEIN; SOMATOMEDIN C; UREA;

ARTICLE; CELL INCLUSION; CELL MEMBRANE PERMEABILITY; CONTAMINATION; CYTOPLASM; ESCHERICHIA COLI; KINETICS; NONHUMAN; PROTEIN DETERMINATION; VISCOSITY;

BIOCHEMISTRY; CELL MEMBRANE PERMEABILITY; DITHIOTHREITOL; EDETIC ACID; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; INCLUSION BODIES; INSULIN-LIKE GROWTH FACTOR I; KINETICS; RECOMBINANT PROTEINS; UREA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032549134     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980220)57:4<381::AID-BIT1>3.0.CO;2-I     Document Type: Article

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