Previously reported nerve growth factor levels are underestimated due to an incomplete release from receptors and interaction with standard curve media

Brain Research

Volumn 842, Issue 1, 1999, Pages 206-210

  Fawcett, John R ^a   Chen, Xueqing ^a,b   Rahman, Yueh Erh ^b   Frey II, William H ^a  

^a Alzheimer's Research Center   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Brain; Endogenous level; Enzyme linked immunosorbent assay; Nerve growth factor

Indexed keywords

NERVE GROWTH FACTOR;

ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACTION; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; RAT; TISSUE DISTRIBUTION; TISSUE LEVEL;

ANIMALS; ANTIBODIES, MONOCLONAL; BRAIN CHEMISTRY; CULTURE MEDIA; ENZYME-LINKED IMMUNOSORBENT ASSAY; MALE; NERVE GROWTH FACTORS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, NERVE GROWTH FACTOR; RECOMBINANT PROTEINS;

EID: 0033581339     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(99)01817-X     Document Type: Article

References (12)

1. Chen X.Q., Fawcett J.R., Rahman Y.R., Ala T.A., Frey W.H. II Delivery of nerve growth factor to the brain via the olfactory pathway. Journal of Alzheimer's Disease. 1:1998;35-44.
2. Henriksson B.G., Soderstrom S., Gower A.J., Ebendal T., Winblad B., Mohammed A.H. Hippocampal nerve growth factor levels are related to spatial learning ability in aged rats. Behav. Brain Res. 48:1992;15-20.
3. Hoener M.C., Hewitt E., Conner J.M., Costello J.W., Varon S. Nerve growth factor (NGF) content in adult rat brain tissue is several-fold higher than generally reported and is largely associated with sedimentable fractions. Brain Res. 728:1996;47-56.
4. Korsching S., Auburger G., Heumann R., Scott J., Thoenen H. Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation. EMBO J. 4:1985;1389-1393.
5. Large T.H., Bodary S.C., Clegg D.O., Weskamp G., Otten U., Reichardt L.F. Nerve growth factor gene expression in the developing rat brain. Science. 234:1986;352-355.
6. Larkfors L., Ebendal T. Highly sensitive enzyme immunoassays for β-nerve growth factor. J. Immunol. Methods. 97:1987;41-47.
7. Linder M., Dworetzky S.I., Sampson C., Loy R. Relationship of APP mRNA transcripts and levels of NGF and low-affinity NGF receptors to behavioral measures of age-related cognitive dysfunction. J. Neurosci. 14:1994;2282-2289.
8. Saffran B.N., Woo J.E., Mobley W.C., Crutcher K.A. Intraventricular NGF infusion in the mature rat brain enhances sympathetic innervation of cerebrovascular targets but fails to elicit sympathetic ingrowth. Brain Res. 492:1989;245-254.
9. Scott S.A., Liang S., Weingartner J.A., Crutcher K.A. Increased NGF-like activity in young but not aged hippocampus after septal lesions. Neurobiol. Aging. 15:1994;337-346.
10. Shozuhara H., Onodera H., Katoh-Semba R., Katoh K., Yamasaki Y., Kagure K. Temporal profiles of nerve growth factor β-subunit level in rat brain regions after transient ischemia. J. Neurochem. 59:1992;175-180.
11. Weskamp G., Otten U. An enzyme-linked immunoassay for nerve growth factor (NGF): a tool for studying regulatory mechanisms involved in NGF production in brain and in peripheral tissues. J. Neurochem. 48:1987;1779-1786.
12. Whittemore S.R., Ebendal T., Larkfors L., Olson L., Seiger A., Stromberg I., Persson H. Developmental and regional expression of β nerve growth factor messenger RNA and protein in the rat central nervous system. Proc. Natl. Acad. Sci. U.S.A. 83:1986;817-821.