An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat

Gene

Volumn 229, Issue 1-2, 1999, Pages 59-65

  Baron, G S ^a   Nano, F E ^a  

^a UNIVERSITY OF VICTORIA   (Canada)

Author keywords

ermC'; F. novicida; MglA; Ori(fd); Reporter gene; TnMaxErCm

Indexed keywords

BACTERIAL DNA; CHLORAMPHENICOL ACETYLTRANSFERASE; ERYTHROMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; DNA REPLICATION; ESCHERICHIA COLI; FRANCISELLA; GENE EXPRESSION REGULATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE;

BACTERIAL PROTEINS; CHLORAMPHENICOL O-ACETYLTRANSFERASE; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DRUG RESISTANCE; ERYTHROMYCIN; FRANCISELLA; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GENETIC MARKERS; MUTAGENESIS, INSERTIONAL; PLASMIDS; TRANSCRIPTION, GENETIC;

EID: 0033580424     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00032-3     Document Type: Article

References (25)

1. Anthony L.S.D., Burke R.D., Nano F.E. Growth of Francisella spp. in rodent macrophages. Infect. Immun. 59:1991;3291-3296.
2. Baron, G.S., 1998. Isolation and characterization of two genetic loci from the intracellular pathogen Francisella novicida, Ph.D. thesis, The University of Victoria, Victoria, Canada.
3. Baron G.S., Nano F.E. MglA and MglB are required for the intramacrophage growth of Francisella novicida. Mol. Microbiol. 29:1998;247-259.
4. Beck E., Zink B. Nucleotide sequence and genome organisation of filamentous bacteriophages f1 and fd. Gene. 16:1981;35-58.
5. Bullock W.O., Fernandez J.M., Short J.M. A high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques. 5:1987;376-378.
6. Chamberlain R.E. Evaluation of live tularemia vaccine prepared in a chemically defined medium. Appl. Microbiol. 13:1965;232-235.
7. Danaher R.J., Petricoin E.F. III, Stein D.C. Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked. J. Bacteriol. 176:1994;3428-3432.
8. Dhandayuthapani S., Via L.E., Thomas C.A., Horowitz P.M., Deretic D., Deretic V. Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages. Mol. Microbiol. 17:1995;901-912.
9. Elhai J., Wolk C.P. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene. 68:1988;119-138.
10. Gorman C.M., Moffat L.F., Howard B.H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2:1982;1044-1051.
11. Haas R., Kahrs A.F., Facius D., Allmeier H., Schmitt R., Meyer T.F. TnMax - a versatile mini-transposon for the analysis of cloned genes and shuttle mutagenesis. Gene. 130:1993;23-31.
12. Kahrs A.F., Bihlmaier A., Facius D., Meyer T.F. Generalized transposon shuttle mutagenesis in Neisseria gonorhoeae: A method for isolating epithelial cell invasion-defective mutants. Mol. Microbiol. 12:1994;819-831.
13. Kahrs A.F., Odenbreit S., Schmitt W., Heuermann D., Meyer T.F., Haas R. An improved TnMax mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions. Gene. 167:1995;53-57.
14. Mahan M.J., Tobias J.W., Slauch J.M., Hanna P.C., Collier R.J., Mekalanos J.J. Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc. Natl. Acad. Sci. USA. 92:1995;669-673.
15. Mdluli K.E., Anthony L.S.D., Baron G.S., McDonald M.K., Myltseva S.V., Nano F.E. Serum-sensitive mutation of Francisella novicida: association with an ABC transporter gene. Microbiology. 140:1994;3309-3318.
16. Mead D.A., Szczesna-Skorupa E., Kemper B. Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Prot. Eng. 1:1986;67-74.
17. Pettersson J., Nordfelth R., Dubinina E., Bergman T., Gustafsson M., Magnusson K.E., WolfWatz H. Modulation of virulence factor expression by pathogen target cell contact. Science. 273:1996;1231-1233.
18. Reilly T.J., Baron G.S., Nano F.E., Kuhlenschmidt M.S. Characterization and sequencing of a respiratory burst-inhibiting acid phosphatase from Francisella tularensis. J. Biol. Chem. 271:1996;10973-10983.
19. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual. 2nd ed. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Silhavy T.J., Beckwith J.R. Uses of lac fusions for the study of biological problems. Microbiol. Rev. 49:1985;398-418.
21. Sleigh M.J. A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells. Analyt. Biochem. 156:1986;251-256.
22. Staendner L.H., Rohde M., Timmis K.N., Guzmán C.A. Identification of Salmonella typhi promoters activated by invasion of eukaryotic cells. Mol. Microbiol. 18:1995;891-902.
23. Williams M.D., Ouyang T.X., Flickinger M.C. Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation. Mol. Microbiol. 11:1994;1029-1043.
24. Zabarovsky E.R., Allikmets R.L. An improved technique for the efficient construction of gene libraries by partial filling-in of cohesive ends. Gene. 42:1986;119-123.
25. Zhou D., Apicella M.A. Plasmids with erythromycin resistance and catechol 2,3-dioxygenase- or β-galactosidase-encoding gene cassettes for use in Neisseria spp. Gene. 171:1996;133-134.