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84920307544
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note
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4OH, 55 °C, 12 h) and 2'-0-deprotected under mild acidic conditions.
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24
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84920307543
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note
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5 The HH16 cleavage reaction is not inhibited by the presence of the fluorescein moiety, and the ability of neomycin to inhibit the reaction is not affected (data not shown).
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25
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84920307542
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note
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HH16-F in the presence or absence of neomycin (up to 10 mM) (under conditions as stated in Figure 2) was examined on a 4% non-denaturing polyacrylamide gel. There was no evidence of either free R16 or S16-F on the gel by visualizing the fluorescein fluorescence or ethidium bromide staining, whereas the HH16-F band was clearly visible and shifted slightly in the presence of neomycin (data not shown).
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84920307541
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note
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5 for determination of neomycin inhibition of HH16 were used.
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