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Volumn 9, Issue 2, 1999, Pages 213-216

RNA-aminoglycoside antibiotic interactions: Fluorescence detection of binding and conformational change

Author keywords

[No Author keywords available]

Indexed keywords

NEOMYCIN; RIBOZYME;

EID: 0033579844     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(98)00718-5     Document Type: Article
Times cited : (39)

References (28)
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    • Von Ahsen, U.1    Davies, J.2    Schroeder, R.3
  • 18
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    • Bassi, G. S.; Møllegaard, N.-E.; Murchie, A. I. H.; von Kitzing, E.; Lilley, D. M. J. Struct. Biol. 1995, 2, 45; Bassi, G. S.; Murchie, A. I. H.; Walter, F.; Clegg, R. M.; Lilley, D. M. J. EMBO J. 1997, 16, 7481; Menger, M.; Tuschl, T.; Eckstein, F.; Porschke, D. Biochemistry 1996, 35, 14710.
    • (1997) EMBO J. , vol.16 , pp. 7481
    • Bassi, G.S.1    Murchie, A.I.H.2    Walter, F.3    Clegg, R.M.4    Lilley, D.M.J.5
  • 19
    • 0030448223 scopus 로고    scopus 로고
    • Bassi, G. S.; Møllegaard, N.-E.; Murchie, A. I. H.; von Kitzing, E.; Lilley, D. M. J. Struct. Biol. 1995, 2, 45; Bassi, G. S.; Murchie, A. I. H.; Walter, F.; Clegg, R. M.; Lilley, D. M. J. EMBO J. 1997, 16, 7481; Menger, M.; Tuschl, T.; Eckstein, F.; Porschke, D. Biochemistry 1996, 35, 14710.
    • (1996) Biochemistry , vol.35 , pp. 14710
    • Menger, M.1    Tuschl, T.2    Eckstein, F.3    Porschke, D.4
  • 21
    • 0029073091 scopus 로고
    • Pley, H. W.; Flaherty, K. M.; McKay, D. B. Nature 1994, 372, 68; Scott, W. G.; Finch, J. T.; Klug, A. Cell 1995, 81, 991.
    • (1995) Cell , vol.81 , pp. 991
    • Scott, W.G.1    Finch, J.T.2    Klug, A.3
  • 23
    • 84920307544 scopus 로고    scopus 로고
    • note
    • 4OH, 55 °C, 12 h) and 2'-0-deprotected under mild acidic conditions.
  • 24
    • 84920307543 scopus 로고    scopus 로고
    • note
    • 5 The HH16 cleavage reaction is not inhibited by the presence of the fluorescein moiety, and the ability of neomycin to inhibit the reaction is not affected (data not shown).
  • 25
    • 84920307542 scopus 로고    scopus 로고
    • note
    • HH16-F in the presence or absence of neomycin (up to 10 mM) (under conditions as stated in Figure 2) was examined on a 4% non-denaturing polyacrylamide gel. There was no evidence of either free R16 or S16-F on the gel by visualizing the fluorescein fluorescence or ethidium bromide staining, whereas the HH16-F band was clearly visible and shifted slightly in the presence of neomycin (data not shown).
  • 26
    • 84920307541 scopus 로고    scopus 로고
    • note
    • 5 for determination of neomycin inhibition of HH16 were used.


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