Preparative enzymatic synthesis and characterization of the cytoplasmic intermediates of murein biosynthesis

Journal of the American Chemical Society

Volumn 121, Issue 6, 1999, Pages 1175-1178

  Reddy, Sreelatha G ^a   Waddell, Sherman T ^a   Kuo, David W ^a   Wong, Kenny K ^a   Pompliano, David L ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PEPTIDOGLYCAN;

ARTICLE; ENZYME MECHANISM; PURIFICATION; SPECTROSCOPY; SYNTHESIS;

EID: 0033576995     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja983850b     Document Type: Article

References (26)

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6. The capitalized gene name in roman type is used to refer to the gene product throughout (i.e., the gene product of murA is designated MurA).
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23. Commercially available TEAB buffer and triethylamine base both contained a contaminant of the same mass. We suspect that this contaminant is a product of light-activated polymerization of triethylamine that yields a nonvolatile compound. Because of this problem, the TEAB solvent system was not used to purify the murein substrates.
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