Thrombin activates transcription factors Sp1, NF-κB, and CREB: Importance of the use of phosphatase inhibitors during nuclear protein extraction for the assessment of transcription factor DNA-binding activities

Analytical Biochemistry

Volumn 275, Issue 2, 1999, Pages 180-186

  Edmead, Christine ^a   Kanthou, Chryso ^a   Benzakour, Omar ^a  

^a THROMBOSIS RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Dephosphorylation; EMSA; Phosphatase inhibitors; Thrombin; Transcription factors

Indexed keywords

AMINO ACIDS; CHEMICAL ACTIVATION; DNA; ELECTROPHORETIC MOBILITY; EXTRACTION; PHOSPHATASES; TRANSCRIPTION FACTORS;

DEPHOSPHORYLATIONS; DNA BINDING ACTIVITY; ELECTROPHORETIC MOBILITY SHIFT ASSAY; EMSA; EXTRACTION PROTOCOLS; GENE TRANSCRIPTIONS; PHOSPHATASE INHIBITORS; THROMBIN;

TRANSCRIPTION;

CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NUCLEAR PROTEIN; THROMBIN; TRANSCRIPTION FACTOR SP1;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DEPHOSPHORYLATION; DNA BINDING; ELECTROPHORETIC MOBILITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION;

EID: 0033571705     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4313     Document Type: Article

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