Cysteine biosynthesis in Chlamydomonas reinhardtii. Molecular cloning and regulation of O-acetylserine(thiol)lyase

European Journal of Biochemistry

Volumn 264, Issue 3, 1999, Pages 848-853

  Ravina, Cristina G ^a   Barroso, Consuelo ^a   Vega, José M ^a   Gotor, Cecilia ^a,b  

^a UNIVERSITY OF SEVILLE   (Spain)

^b Centro de Investigaciones Cientificas Isla de la Cartuja   (Spain)

Author keywords

Cysteine biosynthesis; Gene expression; PCR; Sulfur starvation

Indexed keywords

CYSTEINE; CYSTEINE SYNTHASE; SULFUR;

AMINO ACID SEQUENCE; ARTICLE; CHLAMYDOMONAS REINHARDTII; ENZYME ACTIVITY; ENZYME REGULATION; ENZYME STRUCTURE; GENE EXPRESSION; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHLAMYDOMONAS REINHARDTII; CLONING, MOLECULAR; CYSTEINE; CYSTEINE SYNTHASE; DNA PRIMERS; DNA, COMPLEMENTARY; DNA, PROTOZOAN; GENES, PROTOZOAN; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; RNA, MESSENGER; RNA, PROTOZOAN; SEQUENCE HOMOLOGY, AMINO ACID; SULFUR;

CHLAMYDOMONAS REINHARDTII; EMBRYOPHYTA; PROKARYOTA; TRIXIS;

EID: 0033568397     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00676.x     Document Type: Article

References (30)

1. 1. Barroso, C., Romero, L.C., Vega, J.M. & Gotor, C. (1997) Molecular characterization of the sulfur metabolism in plants. Curr. Topics Phytochem. 1, 19-29.
2. 2. Barroso, C., Vega, J.M. & Gotor, C. (1995) A new member of the cytosolic O-acetylserine (thiol) lyase gene family in Arabidopsis thaliana. FEBS Lett. 363, 1-5.
3. 3. Takahashi, H., Yamazaki, M., Sasakura, N., Watanabe, A., Leustek, T., Engler, J.A., Engler, G., Van Montagu, M. & Saito, K. (1997) Regulation of sulfur assimilation in higher plants: a sulfate transporter induced in sulfate-starved roots plays a central role in Arabidopsis thaliana. Proc. Natl Acad. Sci. USA 94, 11102-11107.
4. 4. Nicholson, M.L., Gaasenbeek, M. & Laudenbach, D.E. (1995) Two enzymes together capable of cysteine biosynthesis are encoded on a cyanobacteial plasmid. Mol. Gen. Genet. 247, 623-632.
5. 5. Prieto, J.L., Pérez-Castiñeira, J.R. & Vega, J.M. (1998) Separación y caracterización de tres isoenzimas con actividad O-acetil-L-serina (tiol) Iiasa en Chlamydomonas reinhardtii. In Avances en el Metabolismo del Nitrógeno: de la Fisiología a la Biología Molecular (Vega, J.M., Aparicio, P.J., Castillo, F., Maldonado, J.M., eds), pp. 371-377. Secretariado de Publicaciones Universidad de Sevilla, Seville, Spain.
6. 6. León, J., Romero, L.C. & Galván, F. (1988) Intracellular levels and regulation of O-acetyl-L-serine sulfhydrylase activity in Chlamydomonas reinhardtii. J. Plant Physiol. 132, 618-622.
7. 7. Shimogawara, K., Fujiwara, S., Grossman, A. & Usuda, H. (1998) High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148, 1821-1828.
8. 8. Harris, E.H. (1989) The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use. Academic Press, San Diego, CA.
9. 9. Hutner, S.H., Provolosi, L., Schatz, A. & y Haskins, C.P. (1950) Some approaches to the study of the role of metals in the metabolism of microorganisms. Proc. Am. Phil Soc: 94, 152-170.
10. 10. Davies, J.P., Yildiz, F.H. & Grossman, A. (1996) Sac 1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J. 15, 2150-2159.
11. 11. Nakai, K. & Horton, P. (1999) PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biol. Sci. 24, 34-35.
12. 12. Marco, Y. & Rochaix, J.-D. (1980) Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardtii. Mol. Gen. Genet. 177, 715-723.
13. 13. León, J., Romero, L.C., Galván, F. & Vega, J.M. (1987) Purification and physicochemical characterization of O-acetyl-L-serine sulfhydrylase from Chlamydomonas reinhardtii. Plant Sci. 53, 93-99.
14. 14. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
15. 15. Campbell, W.H. & Gowri, G. (1990) Codon usage in higher plants, green algae, and cyanobacteria. Plant Physiol. 92, 1-11.
16. 16. Kredich, N.M. (1993) Gene regulation of sulfur assimilation. In Sulfur Nutrition and Assimilation in Higher Plants (De Kok, L.J., Stulen, I., Rennenberg, H., Brunold, C.H., Rauser, W.E., eds), pp. 37-47. SPB Academic Publishing, The Hague, the Netherlands.
17. 17. Sager, R. & Ishida, M.R. (1963) Chloroplast DNA in Chlamydomonas. Proc Natl Acad. Sci. USA 50, 725-730.
18. 18. Shen, W.-H. & Hohn, B. (1992) DMSO improves PCR amplification of DNA with complex secondary structure. Trends Genet. 8, 227.
19. 19. Saito, K., Kurosawa, M. & Murakoshi, I. (1993) Determination of a functional lysine residue of a plant cysteine synthase by site-directed mutagenesis, and the evolutionary implications. FEBS Lett. 328, 111-114.
20. 20. Chen, Q. & Silflow, C.D. (1996) Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii. Plant Physiol. 112, 987-996.
21. 21. Hell, R., Bork, C., Bogdanova, N., Frolov, I. & Hauschild, R. (1994) Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O-acetylserine (thiol) lyase from Arabidopsis thaliana. FEBS Lett. 351, 257-262.
22. 22. Saito, K., Tatsuguchi, K., Takagi, Y. & Murakoshi, I. (1994) Isolation and characterization of cDNA that encodes a putative mitochondrionlocalizing isoform of cysteine synthase (O-acetylserine (thiol)lyase) from Spinacia oleracea. J. Biol. Chem. 269, 28187-28192.
23. 23. Barroso, C., Vega, J.M. & Gotor, C. (1998) The role of roots in cysteine biosynthesis by Arabidopsis thaliana. J. Physiol. Biochem. 54, 189-194.
24. 24. Yildiz, F.H., Davies, J.P. & Grossman, A.R. (1994) Characterization of sulfate transporter in Chlamydomonas reinhardtii during sulfur-limited and sulfur-sufficient growth. Plant Physiol. 104, 981-987.
25. 25. Yildiz, F.H., Davies, J.P. & Grossman, A.R. (1996) Sulfur availability and the Sac 1 gene control adenosine triphosphate sulfurylase gene expression in Chlamydomonas reinhardtii. Plant Physiol. 112, 669-675.
26. 26. Noji, M., Inoue, K., Kimura, N., Gouda, A. & Saito, K. (1998) Isoform-dependent differences in feedback regulation and subcellular localization of serine acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana. J. Biol. Chem. 273, 32739-32745.
27. 27. Saito, K., Miura, N., Yamazaki, M., Hirano, H. & Murakoshi, I. (1992) Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase. Proc. Natl Acad. Sci. USA 89, 8078-8082.
28. 28. Rolland, N., Droux, M., Lebrun, M. & Douce, R. (1993) O-Acetylserine (thiol) lyase from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization, and overexpression in Escherichia coli of the chloroplast isoform. Arch. Biochem. Biophys. 300, 213-222.
29. 29. Byrne, C.R., Monroe, R.S., Ward, K.A. & Kredich, N.M. (1988) DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH. J. Bacteriol. 170, 3150-3157.
30. 30. Sirko, A., Hryniewicz, M., Hulanicka, D. & Böck, A. (1990) Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster. J. Bacetiol. 172, 3351-3357.