Evidence against a major role for Ca2+ in hypoxia-induced gene expression in human hepatoma cells (Hep3B)

Journal of Physiology

Volumn 517, Issue 3, 1999, Pages 651-657

  Metzen, Eric ^a   Fandrey, Joachim ^b   Jelkmann, Wolfgang ^b  

^a UNIVERSITY OF LÜBECK   (Germany)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; EGTAZIC ACID; ERYTHROPOIETIN; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; IONOMYCIN; THAPSIGARGIN; VASCULOTROPIN; BAPTA AM; CHELATING AGENT; EGTA AM; HYPOXIA INDUCIBLE FACTOR 1ALPHA; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM CELL LEVEL; CELL HYPOXIA; CELL LINE; EXTRACELLULAR CALCIUM; GENE EXPRESSION; HEPATOMA CELL; HUMAN; HUMAN CELL; MODEL; PRIORITY JOURNAL; CONTROLLED STUDY; HEP3B CELL LINE; HEPATOCELLULAR CARCINOMA CELL LINE; PROTEIN SECRETION; PROTEIN SYNTHESIS;

BLOTTING, NORTHERN; CALCIUM; CALCIUM-TRANSPORTING ATPASES; CARCINOMA, HEPATOCELLULAR; CELL HYPOXIA; CHELATING AGENTS; EGTAZIC ACID; ENDOTHELIAL GROWTH FACTORS; ENZYME INHIBITORS; ERYTHROPOIETIN; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IONOMYCIN; KINETICS; LIVER NEOPLASMS; LYMPHOKINES; THAPSIGARGIN; TUMOR CELLS, CULTURED; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 0033564837     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-7793.1999.0651s.x     Document Type: Article

References (33)

1. ANNABLE, L., COTES, P. M. & MUSSETT, M. V. (1972). The second international reference preparation of erythropoietin, human, urinary, for bioassay. Bulletin of the World Health Organization 47, 99-112.
2. BAE, M. K., KWON, Y. W., KIM, M. S., BAE, S. K., BAE, M. H., LEE, V. M., KIM, Y. J. & KIM, K. W. (1998). Identification of genes differentially expressed by hypoxia in hepatocellular carcinoma cells, Biochemical and Biophysical Research Communications 243, 158-162.
3. BRITIGAN, B. E., RASMUSSEN, G. T. & Cox, C. D. (1998). Binding of iron and inhibition of iron-dependent oxidative cell injury by the 'calcium chelator' 1,2-bis (2-aminophenoxy)-ethane N, N, N′, N′-tetraacetic acid (BAPTA). Biochemical Pharmacology 55, 287-295.
4. BUNN, H. F. & POYTON, R. O. (1996). Oxygen sensing and molecular adaptation to hypoxia. Physiological Reviews 76, 839-885.
5. CHOMCZYNSKI, P. & SACCHI, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162, 156-159.
6. FANDREY, J. & BUNN, H. F. (1993). In vivo and in vitro regulation of erythropoietin mRNA: Measurement by competitive polymerase chain reaction. Blood 81, 617-623.
7. FANDREY, J., FREDE, S., EHLEBEN, W., PORWOL, T., ACKER, H. & JELKMANN, W. (1997). Cobalt chloride and desferrioxamine antagonize the inhibition of erythropoietin production by reactive oxygen species. Kidney International 51, 492-496.
8. FERRARA, N. & DAVIS-SMYTH, T. (1997). The biology of vascular endothelial growth factor. Endocrine Reviews 18, 4-25.
9. 2+ release mechanisms independently of low molecular weight messengers. Journal of Biological Chemistry 272, 28901-28905.
10. GERICKE, M., DROOGMANS, G. & NILIUS, B. (1993). Thapsigargin discharges intracellular calcium stores and induces transmembrane currents in human endothelial cells. Pflügers Archiv 422, 552-557.
11. GLEADLE, J. M. & RATCLIFFE, P. J. (1998). Hypoxia and the regulation of gene expression. Molecular Medicine Today 4, 122-129.
12. GOLDBERG, M. A., DUNNING, S. P. & BUNN, H. F. (1988). Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein. Science 242, 1412-1415.
13. GOLDBERG, M. A., GLASS, G. A., CUNNINGHAM, J. M. & BUNN, H. F. (1987). The regulated expression of erythropoietin by two human hepatoma cell lines. Proceedings of the National Academy of Sciences of the USA 84, 7972-7976.
14. GOLDBERG, M. A. & SCHNEIDER, T. J. (1994). Similarities between the oxygen-sensing mechanisms regulating the expression of vascular endothelial growth factor and erythropoietin. Journal of Biological Chemistry 269, 4355-4359.
15. JELKMANN, W. & METZEN, E. (1996). Erythropoietin in the control of red cell production. Annals of Anatomy 178, 391-403.
16. 2+ exchange current in isolated toad pacemaker cells. Journal of Physiology 508, 153-166.
17. 2+ pump protein of sarcoplasmic reticulum. Animal of Biological Chemistry 266, 22912-22918.
18. KIMBALL, S. R. & JEFFERSON, L. S. (1992). Regulation of protein synthesis by modulation of intracellular calcium in rat liver. American Journal of Physiology 263, E958-964.
19. 1-Antitrypsin. Journal of Biological Chemistry 268, 2001-2008.
20. MAXWELL, P. H., PUGH, C. W. & RATCLIFFE, P. J. (1993). Inducible operation of the erythropoietin 3́ enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. Proceedings of the National Academy of Sciences of the USA 90, 2423-2427.
21. 2 consumption in monolayer cell cultures. Respiration Physiology 100, 101-106.
22. MORGAN, J. I. & CURRAN, T. (1986). Role of ion flux in the control of c-fos expression. Nature 322, 552-555.
23. 1 inhibits hypoxia-induced vascular permeability factor (vascular endothelial growth factor) gene expression. Biochemical and Biophysical Research Cummunications 229, 733-738.
24. NAGAKURA, K., UENO, M., BROOKINS, J., BECKMAN, B. S. & FISHER, J. W. (1987). Effects of low calcium levels on erythropoietin production by human renal carcinoma cells in culture. American Journal of Physiology 253, C797-801.
25. ODA, K. (1992). Calcium depletion blocks proteolytic cleavages of plasma protein precursors which occur at the golgi and/or transgolgi network. Journal of Biological Chemistry 267, 17465-17471.
26. PAREKH, A. B. & PENNEH, H. (1995). Depletion-activated calcium current is inhibited by protein kinase in RBL-2H3 cells. Proceedings of the National Academy of Sciences of the USA 92, 7907-7911.
27. PORWOL, T., EHLEBEN, W., ZIEROLD, K., FANDHEY, J. & ACKER, H. (1998). The influence of nickel and cobalt on putative members of the oxygen-sensing pathway of erythropoietin-producing HepG2 cells. European Journal of Biochemistry 256, 16-23.
28. 2+ exchange in vascular smooth muscle cells. Cell Calcium 10, 125-134.
29. WANG, G. L. & SEMENZA, G. L. (1996). Oxygen sensing and response to hypoxia by mammalian cells. Redox Report 2, 89-96.
30. WENGER, R. H. & GASSMANN, M. (1997). Oxygen(es) and the hypoxia-inducible factor-1. Biological Chemistry 378, 609-616.
31. WIESENER, M. S., TURLEY, H., ALLEN, W. E., WILLAM, C., ECKARDT, K.-U., TALKS, K. L., WOOD, S. M., GATTER, K. C., HARRIS, A. L., PUGH, C. W., RATCLIFFE, P. J. & MAXWELL, P. H. (1998). Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1α, Blood 92, 2260-2268.
32. WODNAR-FILIPOWICZ., R. & MORONI, C. (1990). Regulation of interleukin 3 mRNA expression in mast cells occurs at the posttranscriptional level and is mediated by calcium ions. Proceedings of the National Academy of Sciences of the USA 87, 777-781.
33. WOLFF, M. & JELKMANN, W. (1993). Effects of chemotherapeutic and immunosuppressive drugs on the production of erythropoietin in human hepatoma cultures. Annals of Hematology 66, 27-31.