Apparent heterogeneity of recombinant interferon γ receptors produced in prokaryotic and eukaryotic expression systems

Journal of Chemical Technology and Biotechnology

Volumn 65, Issue 2, 1996, Pages 123-130

  Fountoulakis, Michael ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Interferon receptors; Protein heterogeneity; Recombinant proteins

Indexed keywords

DODECYL SULFATE SODIUM; GAMMA INTERFERON; HYBRID PROTEIN; IMMUNOGLOBULIN G; INTERFERON RECEPTOR; PHOSPHORIC ACID; POLYACRYLAMIDE GEL; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

BACULOVIRUS; CHO CELL; ESCHERICHIA COLI; EUKARYOTE; GENE EXPRESSION; GLYCOSYLATION; INSECT; LIGAND BINDING; PROKARYOTE; PROTEIN POLYMORPHISM; PROTEIN PROCESSING; PULSED FIELD GEL ELECTROPHORESIS; REVIEW; VIRUS INFECTION;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD; BIOTECHNOLOGY; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLYCOSYLATION; HUMANS; MICE; MOLECULAR SEQUENCE DATA; RECEPTORS, INTERFERON; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0030033238     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4660(199602)65:2<123::AID-JCTB400>3.0.CO;2-6     Document Type: Review

References (38)

1. Yan, S. C. B., Grinnell, B. W. & Wold, F., Post-translational modifications of proteins: some problems left to solve. Trends Biochem. Sci., 14 (1989) 264-8.
2. Mohan, S. B., Chohan, S. R., Eade, J. & Lyddiatt, A., Molecular integrity of monoclonal antibodies produced by hybridoma cells in batch culture and in continuous-flow culture with integrated product recovery. Biotechnol. Bioengineering, 42 (1993) 974-86.
3. Mullner, S., Karbe-Thonges, B. & Tripier D., Charge heterogeneity of insulin fusion proteins expressed in Escherichia coli is not due to proteolytic degradation. Anal. Biochem., 210 (1993) 366-73.
4. Goochee, C. F. & Monica, T., Environmental effects on protein glycosylation. Bio/Technology, 8 (1990) 421-7.
5. Manneberg, M., Lahm, H.-W, & Fountoulakis, M., Oxidation of cysteine and methionine residues during acid-hydrolysis of proteins in the presence of sodium azide. Anal. Biochem., 224 (1995) 122-7.
6. Bialy, H., Recombinant proteins: virtual authenticity. Bio/ Technology, 5 (1987) 883-90.
7. Geisow, M. J., Characterizing recombinant proteins. Bio/ Technology, 9 (1991) 921-4.
8. Fountoulakis, M., Juranville, J.-F., Stüber, D., Weibel, E. K. & Garotta, G., Purification and biochemical characterization of a soluble human interferon γ receptor expressed in Echerichia coli. J. Biol. Chem., 265 (1990) 13268-75.
9. Fountoulakis, M., Schlaeger, E.-J., Gentz, R., Juranville, J.-F., Manneberg, M., Ozmen, L. & Garotta, G., Purification and biochemical characterization of a soluble mouse interferon γ receptor produced in insect cells. Eur. J. Biochem., 198 (1991) 441-50.
10. Gentz, R., Hayes, A., Grau, N., Fountoulakis, M., Ozmen L., Lahm, H.-W. & Garotta, G., Analysis of soluble human and mouse interferon γ receptors expressed in eukaryotic cells. Eur. J. Biochem., 210 (1992) 545-54.
11. Kürschner, C., Garotta, G. & Dembic, Z., Construction, purification, and characterization of new interferon γ inhibitor proteins. J. Biol. Chem., 267 (1992) 9354-60.
12. Fountoulakis, M., Mesa, C., Schmid, G., Gentz, R., Manneberg, M., Dembic, Z. & Garotta, G., Interferon γ receptor extracellular domain expressed as IgG fusion protein in Chinese hamster ovary cells. Purification, biochemical characterization and stoichiometry of binding. J. Biol. Chem., 270 (1995) 3958-64.
13. Ozmen, L., Fountoulakls, M. & Garotta, G., A novel solid-phase test to study the binding of IFNγ to its receptor. J. Immunol. Methods, 147 (1992) 261-70.
14. Ozmen, L., Gribaudo, G., Fountoulakis, M., Gentz, R., Landolfo, S & Garotta, G., Mouse soluble IFNγ receptor as IFNγ inhibitor: distribution, antigenicity and activity after injection in mice. J. Immun., 150 (1993) 2698-705.
15. Landolfo, S. & Garotta, G., IFNγ, a lymphokine that modulates imrnunological and inflammatory responses. J. Immunol. Res., 3 (1991) 81-94.
16. Farrar, M. & Schreiber, R. D., The molecular cell biology of interferon γ and its receptor. Annu. Rev. Immunol., 11 (1993) 559-99.
17. Garotta, G., Ozmen, L. & Fountoulakis, M., Development of interferon γ antagonists as an example of biotechnology application to approach new immunomodulators. Pharmacol. Res., 21, Suppl. 2 (1989) 5-17.
18. Aguet, M., Dembic, Z. & Merlin, G., Molecular cloning and expression of the human interferon γ receptor. Cell, 55 (1988) 273-80.
19. Fountoulakis, M., Kania, M., Ozmen, L., Lötscher, H.-R., Garotta, G. & van Loon, A. P. G. M., Structure and membrane topology of the high-affinity receptor for human IFNγ: requirements for binding IFNγ. One single 90-kitodalton IFNγ receptor can lead to multiple cross-linked products and isolated proteins. J. Immunol., 143 (1989) 3266-76.
20. van Loon, A. P. G. M., Ozmen, L., Fountoulakis, M., Kania, M., Haiker, M. & Garotta, G., High-affinity receptor for interferon-gamma (IFNγ), a ubiquitous protein occurring in different molecular forms on human cells: blood monocytes and eleven different cell lines have the same IFNγ receptor protein. J. Leuk. Biol., 49 (1991) 462-73.
21. Soh, J., Donnelly, R. J., Kotenko, S., Mariano, T. M., Cook, J. R., Wang, N., Emanuel, S., Schwartz, B., Miki, T. & Pestka, S., Identification and sequence of an accessory factor required for activation of the human interferon γ receptor. Cell, 76 (1994) 793-802.
22. Hemmi, S., Böhni, R., Stark, G., Di Marco, F. & Aguet, M., A novel member of the interferon receptor family complements functionality of the murine interferon γ receptor in human cells. Cell, 76 (1994) 803-10.
23. Fountoulakis, M. & Juranville, J.-F., Reduced binding capacity of Concanavalin A-Sepharose after treatment with denaturing agents. J. Biochem. Biophys. Methods, 27 (1993) 127-32.
24. Schreiber, R. D. & Aguet, M., The interferon γ receptor. In Guidebood to Cytokines and Their Receptors, ed. N. A. Nicola. Oxford University Press, Oxford, 1994, pp. 120-3.
25. Szkudlinski, M. W., Thotakura, N. R., Bucci, I., Joshi, L. R., Tsai, A., East-Palmer, J., Shiloach, J. & Weintraub, B. D., Purification and characterization of recombinant human thyrotropin (TSH) isoforms produced by Chinese hamster ovary cells: the role of sialylation and sulfation in TSH bioactivity. Endocrinology, 133 (1993) 1490-503.
26. Stüber, D., Matile, H. & Garotta, G., System for high-level production in Escherichia coli and rapid purification of recombinant proteins. Immunol. Methods, 4 (1990) 121-52.
27. Ozmen, L., Fountoulakis, M., Stüber, D. & Garotta, G., The IFNγ receptor as tool for the discovery of new immunomodulatory drugs. In Developments in Biotherapy: From Clone to Clinic, eds D. J. A. Crommelin & H. Schellekens. Kluwer Academic Publishers, Dordrecht, 1990, Vol. 1, pp. 283-9.
28. Stüber, D., Friedlein, A., Fountoulakis, M., Lahm, H.-W. & Garotta, G., Alignment and disulfide bonds of the extracellular domain of the interferon γ receptor and investigation of their role in biological activity. Biochemistry, 32 (1993) 2423-30.
29. Fountoulakis, M., Unfolding intermediates of the extracellular domain of the interferon γ receptor. J. Biol. Chem., 267 (1992) 7095-100.
30. Fountoulakis, M., Lahm, H.-W., Maris, A., Friedlein, A., Manneberg, M., Stüber, D. & Garotta, G., A 25-kDa stretch of the extracellular domain of the human interferon γ receptor is required for full ligand binding capacity. J. Biol. Chem., 266, (1991) 14970-7.
31. Fountoulakis, M., Vilbois, F., Oesterhelt G. & Vetter, W., Phosphoric acid entrapment leads to apparent protein heterogeneity. Bio/Technology, 13 (1995) 383-8.
32. Chowdhury, S. K., Katta, V., Beavis, R. C. & Chait, B. T., Origin and removal of adducts (molecular mass = 98 u) attached to peptide and protein ions in electrospray ionization mass spectra. J. Am. Soc. Mass Spectrom., 1 (1990) 382-8.
33. Ghatge, M. S. & Deshpande, V. V., Evidence for specific interactions of guanidine hydrochloride with carboxy groups of enzymes/proteins. Biochem. Biophys. Res. Commun., 193 (1993) 979-84.
34. Ding, A., Ojingwa, J. C. McDonach, A. F., Burlingame, A. L. & Benet, L. Z., Evidence for covalent binding of acyl glucuronides to serum albumin via an imine mechanism as revealed by tandem mass spectrometry. Proc. Natl Acad. Sci. USA, 90 (1993) 3797-801.
35. Mesa, C., Dembic, Z., Garotta, G. & Fountoulakis, M., Interferon γ receptor extracellular domain-IgG fusion protein produced in Chinese hamster ovary cells as a mixture of glycoforms. J. Interferon Cytokine Res., 15 (1995) 309-15.
36. Fountoulakis, M. & Gentz, R., Effect of glycosylation on properties of soluble interferon gamma receptors produced in prokaryotic and eukaryotic expression systems. Bio/ Technology, 10 (1992) 1143-7.
37. Takeuchi, M., Noboru, I., Strickland, T. W., Kubota, M., Wada, M., Shimizu, R., Hoshi, S., Kozutsumi, H., Takasaki, S. & Kobata, A., Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. Proc. Natl Acad. Sci., 86 (1989) 7819-22.
38. Manneberg, M., Friedlein, A., Kurt, H., Lahm, H.-W. & Fountoulakis, M., Structural analysis and localization of the oligosaccharide moieties of a soluble interferon γ receptor produced in baculovirus-infected insect cells. Protein Sci., 3 (1994) 30-8.