Methylation of CpGs as a determinant of transcriptional activation at alternative promoters for transforming growth factor-β3

Cancer Research

Volumn 59, Issue 10, 1999, Pages 2292-2296

  Archey, William B ^a   Sweet, Matthew P ^a   Alig, Graham C ^a   Arrick, Bradley A ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BISULFITE; DNA; MESSENGER RNA; RNA; TRANSFORMING GROWTH FACTOR BETA3;

ARTICLE; BREAST CANCER; CANCER CELL; CELL CULTURE; CELL LINE; DNA DETERMINATION; DNA METHYLATION; DNA SEQUENCE; GENOME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; RNA ANALYSIS; SEQUENCE ANALYSIS; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BREAST NEOPLASMS; CELL LINE; CPG ISLANDS; DNA METHYLATION; DNA, NEOPLASM; FEMALE; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; NEOPLASM PROTEINS; ORGAN SPECIFICITY; PROMOTER REGIONS (GENETICS); RNA, MESSENGER; SARCOMA; TRANSCRIPTION, GENETIC; TRANSFORMING GROWTH FACTOR BETA; TUMOR CELLS, CULTURED;

EID: 0033563117     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Comb, M., and Goodman, H. M. CpG methylation inhibits proenkephalin gene expression and binding of the transcription factor AP-2. Nucleic Acids Res., 18: 3975-3982, 1990.
2. Tale, P. H., and Bird, A. P. Effects of DNA moethylation on DNA-binding proteins and gene expression. Curr. Opin. Genet. Dev., 3: 226-231, 1993.
3. Lapidus, R. G., Nass, S. J., Butash, K. A., Parl, F. F., Weitzman, S. A., Graff, J. G., Herman, J. G., and Davidson, N. E. Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction. Cancer Res., 58: 2515-2519, 1998.
4. Lapidus, R. G., Ferguson, A. T., Ottaviano, Y. L., Parl, F. F., Smith, H. S., Weitzman, S. A., Baylin, S. B., Issa, P. J., and Davidson, N. E. Methylation of estrogen and progesterone receptor gene 5′ CpG islands correlates with lack of estrogen and progesterone receptor gene expression in breast tumors. Clin. Cancer Res., 2: 805-810, 1996.
5. Ohtani-Fujita, N., Fujita, T., Aoike, A., Osifchin, N. E., Robbins, P. D., and Sakai, T. CpG methylation inactivates the promoter activity of the human retinoblastoma tumor-suppressor gene. Oncogene, 8: 1063-1067, 1993.
6. Graff, J. R., Herman, J. G., Lapidus, R. G., Chopra, H., Xu, R., Jarrard, D. F., Isaacs, W. B., Pitha, P. M., Davidson, N. E., and Baylin, S. B. E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas. Cancer Res., 55: 5195-5199, 1995.
7. Mancini, D. N., Rodenhiser, D. I., Ainsworth, P. J., O'Malley, F. P., Singh, S. M., Xing, W., and Archer, T. K. CpG methylation within the 5′ regulatory region of the BRCA1 gene is tumor specific and includes a putative CREB binding site. Oncogene, 16: 1161-1169, 1998.
8. Lafyatis, R., Lechleider, R., Kim, S-J., Jakowlew, S., Roberts, A. B., and Sporn, M. B. Structural and functional characterization of the transforming growth factor β3 promoter. A cAMP-responsive element regulates basal and induced transcription. J. Biol. Chem., 265: 19128-19136, 1990.
9. Arrick, B. A., Lee, A. L., Grendell, R. L., and Derynck, R. Inhibition of translation of transforming growth factor-β3 mRNA by its 5′ untranslated region. Mol. Cell. Biol., 11: 4306-4313, 1991.
10. Arrick, B. A., Grendell, R. L., and Griffin, L. A. Enhanced translational efficiency of a novel transforming growth factor-β3 mRNA in human breast cancer cells. Mol. Cell. Biol., 14: 619-628, 1994.
11. Raizis, A. M., Schmitt, F., and Jost, J-P. A bisulfite method of 5-methylcytosine mapping that minimizes template degradation. Anal. Biochem., 226: 161-166, 1995.
12. Zhou, C., Yang, Y., and Jong, A. Y. Mini-prep in ten minutes. BioTechniques, 8: 172-173, 1990.
13. Ayoubi, T. A. Y., and Van de Ven, W. J. M. Regulation of gene expression by alternative promoters. FASEB J., 10: 453-460, 1996.
14. Kozak, M. An analysis of vertebrate mRNA sequences: intimations of translational control. J. Cell Biol., 115: 887-903, 1991.
15. Nielsen, F. C., Gammeltoft, S., and Christiansen, J. Translational discrimination of mRNAs coding for human insulin-like growth factor II. J. Biol. Chem., 265: 13431-13434, 1990.
16. Xie, J., Roddy, P., Rife, T. K., Murad, F., and Young, A. P. Two closely linked but separable promoters for human neuronal nitric oxide synthase gene transcription. Proc. Natl. Acad. Sci. USA, 92: 1242-1246, 1995.
17. Herman, J. G., Graff, J. R., Myöhänen, S., Nelkin, B. D., and Baylin, S. B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. USA, 93: 9821-9826, 1996.
18. Xiong, Z., and Laird, P. W. COBRA: a sensitive and quantitative DNA methylation assay. Nucleic Acids Res., 25: 2532-2534, 1997.