Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation

Yeast

Volumn 15, Issue 1, 1999, Pages 43-50

  Wolff, Edith C ^a   Park, Myung Hee ^a  

^a NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH   (United States)

Author keywords

Deoxyhypusine synthase; EIF5a; Hypusine; Posttranslational modification; Yeast

Indexed keywords

ALANINE; ARGININE; DEOXYHYPUSINE; IMINE; INITIATION FACTOR 5A; LYSINE; SPERMIDINE; SYNTHETASE; DEOXYHYPUSINE SYNTHASE; DRUG DERIVATIVE; HYPUSINE; INITIATION FACTOR; OXIDOREDUCTASE;

ARTICLE; CELL GROWTH; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; EUKARYOTIC CELL; GENE MUTATION; HUMAN; NONHUMAN; PRIORITY JOURNAL; YEAST; AMINO ACID SEQUENCE; ANIMAL; BINDING SITE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; MOUSE; PROTEIN PROCESSING; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; HUMANS; LYSINE; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDOREDUCTASES ACTING ON CH-NH GROUP DONORS; PEPTIDE INITIATION FACTORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SACCHAROMYCES CEREVISIAE;

EID: 0033554888     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19990115)15:1<43::AID-YEA344>3.0.CO;2-K     Document Type: Article

References (26)

1. Abid, M. R., Sasaki, K., Titani, K. and Miyazaki, M. (1997). Biochemical and immunological characterization of deoxyhypusine synthase purified from the yeast Saccharomyces carlsbergensis. J. Biochem. (Tokyo) 121, 769-778.
2. Bartig, D., Schümann, H. and Klink, F. (1990). The unique posttranslational modification leading to deoxyhypusine or hypusine is a general feature of the Archebacterial Kingdom. System. Appl. Microbiol. 13, 112-116.
3. Bult, C. et al. (1996). Complete genome sequence of the methanogenic Archaeon, Methanococcus jannaschii. Science 273, 1058 1073.
4. Byers, T. L., Ganem, B. and Pegg, A. E. (1992). Cytostasis induced in L1210 murine leukemia cells by the S-adenosyl-L-methionine decarboxylase inhibitor 5′-{[(Z)-4-amino-2-butenyl]methylamino}-5′-deoxyadenosine may be due to hypusine depletion. Biochem. J. 287, 717-724.
5. Byers, T. L., Lakanen, J. R., Coward, J. K. and Pegg, A. E. (1994). The role of hypusine depletion in cytostasis induced by S-adenosyl-L-methionine decarboxylase inhibition: new evidence provided by 1-methyl-spermidine and 1,12-dimethylspermine. Biochem. J. 303, 363-368.
6. Gordon, E. D., Mora, R., Meredith, S. C., Lee, C. and Lindquist, S. L. (1987). Eukaryotic initiation factor 4D, the hypusine-containing protein, is conserved among eukaryotes. J. Biol. Chem. 262, 16, 585-16,589.
7. Joe, Y. A., Wolff, E. C. and Park, M. H. (1995). Cloning and expression of human deoxyhypusine synthase cDNA: structure function studies with the recombinant enzyme and mutant proteins. J. Biol. Chem. 270, 22,386-22,392.
8. 329 in human deoxyhypusine synthase. J. Biol. Chem. 272, 32,679-32,685.
9. Kang, K. R., Wolff, E. C., Park, M. H., Folk, J. E. and Chung, S. I. (1995). Identification of YHR068w in Saccharomyces cerevisiae chromosome VIII as a gene for deoxyhypusine synthase: expression and characterization of the enzyme. J. Biol. Chem. 270, 18,408-18,412.
10. Klenk, H-P. et al. (1997). The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon, Archaeoglobus fulgidus. Nature 390, 364-370.
11. Klier, H., Csonga, R., Steinkasser, A., Wöhl, T., Lottspeich, F. and Eder, J. (1995). Purification and characterization of human deoxyhypusine synthase from HeLa cells. FEBS Lett. 364, 207-210.
12. Liao, D.-I., Wolff, E. C., Park, M. H. and Davies, D. R. (1998). Crystal structure of the NAD complex of human deoxyhypusine synthase: an enzyme with a ball and chain mechanism for blocking the active site. Structure 6, 23-32.
13. Park, M. H., Wolff, E. C. and Folk, J. E. (1993). Is hypusine essential for eukaryotic cell proliferation? Trends Biochem. Sci. 18, 475-479.
14. Park, M. H., Lee, Y. B. and Joe, Y. A. (1997). Hypusine is essential for eukaryotic cell proliferation. Biol. Signals 6, 115-123.
15. Park, M. H., Joe, Y. A. and Kang, K. R. (1998). Deoxyhypusine synthase activity is essential for cell viability in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 273, 1677-1683.
16. Sasaki, K., Abid, M. R. and Miyazaki, M. (1996). Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae. FEBS Lett. 384, 151-154.
17. Schnier, J., Schwelberger, H., Smit-McBride, Z., Kang, H. A. and Hershey, J. W. B. (1991). Translation initiation factor 5A and its hypusine modification are essential for cell viability in yeast. Mol. Cell. Biol. 11, 3105-3114.
18. Schümann, H, and Klink, F. (1989). Archaebacterial protein contains hypusine, a unique amino acid characteristic for eukaryotic translation initiation factor 4D. System Appl. Microbiol. 11, 103-107.
19. Schwelberger, H. G., Kang, H. A. and Hershey, J. W. B. (1993). Translation initiation factor eIF-5A expressed from either of two yeast genes or from human cDNA: functional identity under aerobic and anaerobic conditions. J. Biol. Chem. 268, 14,018-14,025.
20. Smith, D. R. et al. (1997). Complete genome sequence of Methanobacterium thermoautotrophicum delta H: functional analysis and comparative genomics. J. Bacteriol. 179, 7135-7155.
21. Tao, Y., Skrenta, H. M. and Chen, K. Y. (1994). Deoxyhypusine synthase assay based on the use of polyhistidine-tagged substrate and metal chelate-affinity chromatography. Anal. Biochem. 221, 103-108.
22. Tao, Y. and Chen, K. Y. (1995a). Purification of deoxyhypusine synthase from Neurospora crassa to homogeneity by substrate elution affinity chromatography. J. Biol. Chem. 270, 383-386.
23. Tao, Y. and Chen, K. Y. (1995b). Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA. J. Biol. Chem. 270, 23,984-23,987.
24. Wöhl, T., Klier, H., Ammer, H., Lottspeich, F. and Magdolen, V. (1993). The HYP2 gene of Saccharomyces cerevisiae is essential for aerobic growth: characterization of different isoforms of the hypusine-containing protein Hyp2p and analysis of gene disruption mutants. Mol. Gen. Genet. 241, 305-311.
25. Wolff, E. C., Lee, Y. B., Chung, S. I., Folk, J. E. and Park, M. H. (1995). Deoxyhypusine synthase from rat testis: purification and characterization. J. Biol. Chem. 270, 8660-8666.
26. Wolff, E. C., Folk, J. E. and Park, M. H. (1997). Enzyme-substrate intermediate formation at lysine 329 in human deoxyhypusine synthase. J. Biol. Chem. 272, 15,865-15,871.