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0344877813
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note
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Hydrolytic stability is of the amide bond. In all of these compounds, the ester group in the side chain hydrolyzed slowly at pH 7, but in separate experiments 10 and the salt of its corresponding free acid (isolated as a minor byproduct) were found to photorelease glutamate with equal efficiency. Ester hydrolysis is thus unimportant. The stabilities quoted are from experiments on the free acids of 9 and 10 and were determined from reverse-phase HPLC measurements of disappearance of starting compounds. The free acids avoided analytical complications from fast cleavage of the ester group.
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23
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0344877811
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2+-free mammalian Ringer's solution containing 100 μM glycine
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2+-free mammalian Ringer's solution containing 100 μM glycine.
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24
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0019441262
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Sigworth, F.L.5
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26
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0344446035
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note
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p values and extinction coefficients, ∼15% conversion of the 1 mM solution of 10 is expected.
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