Synaptic calcium transients in single spines indicate that NMDA receptors are not saturated

Nature

Volumn 399, Issue 6732, 1999, Pages 151-155

  Mainen, Zachary F ^a   Malinow, Roberto ^a   Svoboda, Karei ^a  

^a Watson School of Biological Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPA RECEPTOR; N METHYL DEXTRO ASPARTIC ACID RECEPTOR;

ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CENTRAL NERVOUS SYSTEM; FLUORESCENCE MICROSCOPY; NERVE CELL PLASTICITY; NEUROTRANSMISSION; PRIORITY JOURNAL; RECEPTOR OCCUPANCY; SYNAPTIC TRANSMISSION;

ACTION POTENTIALS; ANIMALS; CALCIUM; DENDRITES; GLUTAMIC ACID; HIPPOCAMPUS; MICROSCOPY, CONFOCAL; N-METHYLASPARTATE; NEURONAL PLASTICITY; NEURONS; RATS; RECEPTORS, N-METHYL-D-ASPARTATE; SYNAPSES;

EID: 0033551327     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/20187     Document Type: Article

References (30)

1. Denk, W. & Svoboda, K. Photon upmanship: why multiphoton imaging is more than a gimmick. Neuron 18, 351-357 (1997).
2. Frerking, M. & Wilson, M. Saturation of postsynaptic receptors at central synapses? Curr. Opin. Neurobiol. 6, 395-403 (1996).
3. Tang, C. M., Margulis M., Sbi, Q. Y. & Fielding, A. Saturation of postsynaptic glutamate receptors after quantal release of transmitter. Neuron 13, 1385-1393 (1994).
4. Auger, C., Kondo, S. & Marty, A. Multivesicular release at single functional synaptic sites in cerebellar stellate and basket cells, J. Neurosci, 18, 4532-4547 (1998).
5. Lester, R. A., Clements, J. D., Westbrook, G. L. & Jahr, C. E. Channel kinetics determine the time course of NMDA receptor-mediated synaptic currents. Nature 346, 565-567 (1990).
6. Clements, J. D., Lester, R. A., Tong, G., Jahr, C. E. & Westbrook, G. L. The time course of glutamate in the synaptic cleft. Science 258, 1498-1501 (1992).
7. Yuste, R. & Denk, W. Dendritic spines as basic functional units of neuronal integration. Nature 375, 682-684 (1995).
8. Harris, K. M. & Stevens, I. K. Dendritic spines of CA1 pyramidal cells in the rat hippocampus: Serial electron microscopy with reference to their biophysical characteristics. J. Neurosci. 9, 2982-2997 (1935).
9. Schikorski, T. & Stevens, C. F. Quantitative ultrastructural analysis of hippocampal excitatory synapses. J. Neurosci. 17, 5858-5867 (1997).
10. Mainen, Z. F. et al. Two-photon imaging in living brain slices. Methods: A Companion to Methods in Enzymology 18 (in the press).
11. Regehr, W. G. & Atluri, P. P. Calcium transients in cerebellar granule cell presynaptic terminals. Biophys. J. 68, 2156-2170 (1995).
12. Markram, H., Helm, P. J. & Sakmann, B. Dendritic calcium transients evoked by single back-propagating action potentials in rat neocortical pyramidal neurons. J. Physiol. (Lond.) 485, 1-20 (1995).
13. Manabe, T. & Nicoll, R. A. Long-term potentiation: evidence against an increase in transmitter release probability in the CA1 region of the hippocampus. Science 265, 1888-1852 (1994).
14. Mainen, Z. F., Iia, Z. P., Roder, J. & Malinow, R. Use-dependent AMPA receptor block in mice lacking GluR2 suggests postsynaptic site for LTP expression. Nature Neurosci, 1, 579-586 (1998).
15. Korn, H., Mallet, A., Triller, A. & Faber, D. S. Transmission at a central inhibitory synapse. II. Quantal description of release, with a physical correlate for binomial n. J. Neurophysiol. 48, 679-707 (1982).
16. Stevens, C. F. & Wang, Y. Facilitation and depression at single central synapses. Neuron 14, 795-802 (1993).
17. Dobrunz, L. E., Huang, E. P. & Stevens, C. F. Very short-term plasticity in hippocampal synapses. Proc. Natl Acad. Sci. USA 94, 14843-14847 (1997).
18. Tong, G. & Jahr, C. E. Multivesicular release from excitatory synapses of cultured hippocampal neurons. Neuron 12, 51-59 (1994).
19. Clements, J. D. Transmitter timecourse in the synaptic cleft: its role in central synaptic function. Trends Neurosci. 19, 163-171 (1996).
20. Rusakov, D. A. & Kullmann, D. M. Extrasynaptic glutamate diffusion in the hippocampus: ultrastructural constraints, uptake, and receptor activation. J. Neurosci. 18, 3158-3170 (1998).
21. Holmes, W. R. Modeling the effect of glutamate diffusion and uptake on NMDA and non-MMDA receptor saturation. Biophys. J. 69, 1734-1747 (1999).
22. Tong, G. & Jahr, C. E. Block of glutamate transporters potentiates postsynaptic excitation. Neuron 13, 1195-1203 (1994).
23. Barbour, B. & Hausser, M. Intersynaptic diffusion of neurotransmitter. Trends Neurosci. 20, 377-384 (1997).
24. Faber, D. S., Young, W. S., Legendre, P. & Korn, H. Intrinsic quantal variability due to stochastic properties of receptor-transmitter interactions. Science 258, 1494-1498 (1992).
25. Silver, R. A., Cull-Candy, S. G. & Takahashi, T. Non-NMDA glutamate receptor occupancy and open probability at a rat cerebellar synapse with single and multiple release sites. J. Physiol. (Lond.) 494, 231-250 (1996).
26. Bliss, T. V. P. & Collingridge, G. L. A synaptic model of memory: Long-term potentiation in the hippocampus. Nature 361, 31-39 (1998)
27. 2+ signaling requirements for long-term depression in the hippocampus Neuron 16, 825-833 (1996).
28. Nowak, L., Bregentovski, P., Archer P., Herbet, A. & Prochiantz, A. Magnesium gates glutamate-activated channels in mouse central neurons. Nature 307, 462-465 (1984).
29. Svoboda, K., Tank, D. W. & Denk, W. Direct measurement of coupling between dendritic spines and shafts. Science 272, 716-719 (1996).
30. 2+ signaling in dendrites of pyramidal neurons. Biophys. J. 70, 1069-1081 (1996).