Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9

Journal of Cell Biology

Volumn 146, Issue 1, 1999, Pages 125-140

  Lehman, Kevin ^a   Rossi, Guendalina ^a   Adamo, Joan E ^a   Brennwald, Patrick ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Cell polarity; Exocytosis; SNARE complex; Tomosyn; Tumor suppressor

Indexed keywords

FUNGAL PROTEIN; MEMBRANE PROTEIN;

ACTIN FILAMENT; ARTICLE; CYTOSKELETON; DROSOPHILA; EXOCYTOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; YEAST;

ACTINS; BETA-FRUCTOFURANOSIDASE; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELL MEMBRANE; CELL POLARITY; COLD; DROSOPHILA PROTEINS; ENDOPLASMIC RETICULUM; EXOCYTOSIS; FUNGAL PROTEINS; GLYCOSIDE HYDROLASES; GOLGI APPARATUS; GTP PHOSPHOHYDROLASES; INSECT PROTEINS; MEMBRANE PROTEINS; MUTATION; NEUROPEPTIDES; PRECIPITIN TESTS; QC-SNARE PROTEINS; RECOMBINANT FUSION PROTEINS; RHO GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TUMOR SUPPRESSOR PROTEINS;

EID: 0033549568     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.146.1.125     Document Type: Article

References (43)

1. Aalto, M.K., H. Ronne, and S. Keranen. 1993. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO (Eur. Mol Biol. Organ.) J. 12:4095-4104.
2. Ayscough, K.R., J. Stryker, N. Pokala, M. Sanders, P. Crews, and D.G. Drubin. 1997. High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A. J. Cell Biol. 137:399-416.
3. Bartel, P., C.T. Chien, R. Sternglanz, and S. Fields. 1993. Elimination of false positives that arise in using the two-hybrid system. Biotechniques. 14:920-924.
4. Becker, D.M., and L. Guarerte. 1991. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194:182-187.
5. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254.
6. Brennwald, P., B. Kearns, K. Champion, S. Keranen, V. Bankaitis, and P. Novick. 1994. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 79:245-258.
7. Durfee, T., K. Becherer, P.L. Chen, S.H. Yeh, Y. Yang, A.E. Kilburn, W.H. Lee, and S.J. Elledge. 1993. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7:555-569.
8. Esmon, B., P. Novick, and R. Schekman. 1981. Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast. Cell. 25:451-460.
9. Fasshauer, D., R.B. Sutton, A.T. Brunger, and R. Jahn. 1998. Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs. Proc. Natl. Acad. Sci. USA. 95:15781-15786.
10. Field, C., and R. Schekman. 1980. Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae. J. Cell Biol. 86:123-128.
11. Fields, S., and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature. 340:245-246.
12. Finger, F.P., and P. Novick. 1997. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol. Biol Cell. 8:647-662.
13. Finger, F.P., T.E. Hughes, and P. Novick. 1998. Sec3p is a spatial landmark for polarized secretion in budding yeast. Cell. 92:559-571.
14. Fujita, Y., H. Shirataki, T. Sakisaka, T. Asakura, T. Ohya, H. Kotani, S. Yokoyama, H. Nishioka, Y. Matsuura, A. Mizoguchi, R.H. Scheller, and Y. Takai. 1998. Tomosyn: a syntaxin-l-binding protein that forms a novel complex in the neurotransmitter release process. Neuron. 20:905-915.
15. Guo, W., D. Roth, C. Walch-Solimena, and P. Novick. 1999. The exocyst is an effector for sec4p, targeting secretory vesicles to sites of exocytosis. EMBO (Eur. Mol. Biol. Organ.) J. 18:1071-1080.
16. Imai, J., A. Toh-e, and Y. Matsui. 1996. Genetic analysis of the Saccharomyces cerevisiae RHO3 gene, encoding a rho-type small GTPase, provides evidence for a role in bud formation. Genetics. 142:359-369.
17. Kagami, M., A. Toh-e, and Y. Matsui. 1998. Sro7p, a Saccharomyces cerevisiae counterpart of the tumor suppressor l(2)gl protein, is related to myosins in function. Genetics. 149:1717-1727.
18. Katz, L., P.I. Hanson, J.E. Heuser, and P. Brennwald. 1998. Genetic and morphological analyses reveal a critical interaction between the C-termini of two SNARE proteins and a parallel four helical arrangement for the exocytic SNARE complex. EMBO (Eur. Mol. Biol. Organ.) J. 17:6200-6209.
19. Lane, D., and E. Harlow. 1988. Antibodies: A Laboratory Manual. Vol 13. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY. 726 pp.
20. Lillie, S.H., and S.S. Brown. 1994. Immunofluorescence localization of the unconventional myosin, Myo2p. and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae. J. Cell Biol. 125:825-842.
21. Masuda, E.S., B.C. Huang, J.M. Fisher, Y. Luo, and R.H. Scheller. 1998. Tomosyn binds t-SNARE proteins via a VAMP-like coiled coil. Neuron. 21:479-480.
22. Matsui, Y., and E.A. Toh. 1992. Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1. Mol. Cell Biol. 12:5690-5699.
23. Mechler, B.M., W. McGinnis, and W.J. Gehring. 1985. Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster. EMBO (Eur. Mol. Biol. Organ.) J. 4:1551-1557.
24. Nair, J., H. Muller, M. Peterson, and P. Novick. 1990. Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy-terminal domain. J. Cell Biol. 110:1897-1909.
25. Neer, E.J., C.J. Schmidt, R. Nambudripar and T.F. Smith. 1994. The ancient regulatory-protein family of WD-repeat proteins. Nature. 371:297-300.
26. Novick, P., and D. Botstein. 1985. Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell. 40:405-416.
27. Novick, P., C. Field, and R. Schekman. 1980. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell. 21:205-215.
28. Protopopov, V., B. Govindan, P. Novick, and J.E. Gerst. 1993. Homologs of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S cerevisiae. Cell. 74:855-861.
29. Pruyne, D.W., D.H. Schott, and A. Bretscher. 1998. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J. Cell Biol. 143:1931-1945.
30. Redding, K., C. Holcomb, and R.S. Fuller. 1991. Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae. J. Cell Biol. 113:527-538.
31. Robinson, N.G., L. Guo, J. Imai, A. Toh-e. Y. Matsui, and F. Tamanoi. 1999. Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with myo2 and exo70. Mol. Cell Biol. 19:3580-3587.
32. Rossi, G., A. Salminen, L.M. Rice, A.T. Brunger, and P. Brennwald. 1997. Analysis of a yeast SNARE complex reveals remarkable similarity to the neuronal SNARE complex and a novel function for the C terminus of the SNAP-25 homolog. Sec9. J. Biol Chem. 272:16610-16617.
33. Sherman, F., G.R. Fink, and J.B. Hicks. 1986. Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 186 pp.
34. Strand, D., R. Jakobs, G. Merdes, B. Neumann, A. Kalmes, H.W. Heid, I. Husmann, and B.M. Mechler. 1994a. The Drosophila lethal(2)giant larvae tumor suppressor protein forms homo-oligomers and is associated with nonmuscle myosin II heavy chain. J. Cell Biol. 127:1361-1373.
35. Strand, D., I. Raska, and B.M. Mechler. 1994b. The Drosophila lethal(2)gianl larvae tumor suppressor protein is a component of the cytoskeleton. J. Cell Biol. 127:1345-1360.
36. TerBush, D.R., T. Maurice, D. Roth, and P. Novick. 1996. The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO (Eur. Mol. Biol. Organ.) J. 15:6483-6494.
37. Tkacz, J.S., and J.O. Lampen. 1972. Wall replication in Saccharomyces species: use of fluorescein-conjugated concanavalin A to reveal the site of mannan insertion. J. Gen. Microbiol. 72:243-247.
38. Tkacz, J.S., and J.O. Lampen. 1973. Surface distribution of invertase on growing Saccharomyces cells. J. Bacteriol. 113:1073-1075.
39. Valls, L.A., C.P. Hunter, J.H. Rothman, and T.H. Stevens. 1987. Protein sorting in yeast: the localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide. Cell. 48:887-897.
40. Walch-Solimena, C., R.N. Collins, and P.J. Novick. 1997. Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles. J. Cell Biol. 137:1495-1509.
41. Walworth, N.C., and P.J. Novick. 1987. Purification and characterization of constitutive secretory vesicles from yeast. J. Cell Biol. 105:163-174.
42. Walworth, N.C., P. Brennwald, A.K. Kabcenell, M. Garrett, and P. Novick. 1992. Hydrolysis of GTP by Sec4 protein plays an important role in vesicular transport and is stimulated by a GTPase-activating protein in Saccharomyces cerevisiae. Mol. Cell Biol. 12:2017-2028.
43. Yokoyama, S., H. Shirataki, T. Sakisaka, and Y. Takai. 1999. Three splicing variants of tomosyn and identification of their syntaxin-binding region. Biochem. Biophys. Res. Commun. 252:218-222.