Identification of a nuclear transport inhibitory signal (NTIS) in the basic domain of HIV-I Vif protein

Journal of Molecular Biology

Volumn 289, Issue 3, 1999, Pages 431-437

  Friedler, Assaf ^a   Zakai, Nehama ^a   Karni, Orit ^a   Friedler, Dorit ^a   Gilon, Chaim ^a   Loyter, Abraham ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

HIV 1; NLS; Nuclear transport; Vif

Indexed keywords

BOVINE SERUM ALBUMIN; VIF PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL NUCLEUS; HUMAN IMMUNODEFICIENCY VIRUS 1; INTRACELLULAR TRANSPORT; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; SIMIAN VIRUS 40; VIRUS INFECTION;

BOVINAE; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 0033546118     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.2785     Document Type: Article

References (36)

1. Beg, A. A., Ruben, S. M, Scheinman, R. I., Haskill, S., Rosen, C. A. & Baldwin, A. S., Jr (1992). IkB interacts with the nuclear localization sequences of the subunits of NF-kB: A mechanism for cytoplasmic retention. Genes Dev. 6, 1899-1913.
2. Broder, Y. C., Stanhill, A., Zakai, N., Friedler, A. C., Gilon, & Loyter, A. (1997). Translocation of NLS-BSA conjugates into nuclei of permeabilized mammalian cells can be supported by protoplast extract: An experimental system for studying plant cytosolic factors involved in nuclear import. FEBS Letters, 412, 535-539.
3. Bukrinsky, M. I., Haggerty, S., Dempsey, M. P., Sharova, N., Adzhubel, A., Spitz, L., Lewis, P., Goldfarb, D., Emerman, M. & Stevenson, M. (1993). A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells. Nature, 365, 666-669.
4. Chowdhury, L. H., Chao, W., Potash, M. J., Sova, P., Gendelman, H. E. & Volsky, D. J. (1996). Vif-negative human immunodeficiency virus type 1 persistently replicates in primary macrophages producing attenuated progeny virus. J. Virol. 70, 5336-5345.
5. Cochrane, A. W., Perkins, A. & Rosen, C. A. (1990). Identification of sequences important in the nucleolar localization of human immunodefiency virus rev: Relevance of nucleolar localization to function. J. Virol. 64, (2), 881-885.
6. Conti, E., Uy, M., Leighton, L., Blobel, G. & Kuriyan, J. (1998). Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α. Cell, 94, 193-204.
7. Cullen, B. R. (1998). HIV-1 auxiliary proteins: Making connections in a dying cell. Cell, 93, 685-692.
8. Dingwall, C. & Laskey, R. A. (1991). Nuclear targeting sequences-a concensus? Trends Biochem. Sci. 16, 478-481.
9. Efthymiadis, A., Briggs, L. J. & Jans, D. A. (1998). The HIV-1 Tat protein nuclear localization sequence confers novel nuclear import properties. J. Biol. Chem. 273, (3), 1623-1628.
10. Friedler, A., Zakai, N., Karni, O., Broder, Y. C., Baraz, L., Kotler, M., Loyter, A. & Gilon, C. (1998). Backbone cyclic peptide, which mimics the nuclear localization signal of HIV-1 matrix protein, inhibits nuclear import and virus production in non-dividing cells. Biochemistry, 37(16), 5616-5622.
11. Gallay, P., Stitt, V., Mundy, C., Oettinger, M. & Trono, D. (1996). Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import. J. Virol. 70, (2), 1027-1032.
12. Gallay, P., Hope, T., Chin, D. & Trono, D. (1997). HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway. Proc. Natl Acad. Sci. USA, 94, 9825-9830.
13. Gao, M. & Knipe, D. M. (1992). Distal protein sequences can affect the function of a nuclear localization signal. Mol. Cell. Biol. 12, (3), 1330-1339.
14. Garcia-Bustos, J., Heitman, J. & Hall, M. N. (1991). Nuclear protein localization. Biochim. Biophys. Acta, 1071, 83-101.
15. Goncalves, J., Jallepalli, P. & Gabuzda, D. H. (1994). Subcellular localization of the vif protein of human immunodeficiency virus type 1. J. Virol. 68, (2), 704-712.
16. Gorlich, D. (1998). Transport into and out of the cell nucleus. EMBO J. 17, (10), 2721-2727.
17. Gorlich, D., Prehn, S., Laskey, R. A. & Hartmann, E. (1994). Isolation of a protein that is essential for the first step of nuclear protein import. Cell, 79, 767-778.
18. Gorlich, D., Vogel, E., Mills, A. D., Hartmann, E. & Laskey, R. A. (1995). Distinct functions for the two importin subunits in nuclear protein import. Nature, 377, 246-248.
19. Heinzinger, N. K., Bukrinsky, M. I., Haggerty, S. A., Ragland, A. M., Kewalraman, V., Lee, M. A., Gendelman, H. E., Ratner, L., Stevenson, M. & Emerman, M. (1994). The Vpr protein of HIV-1 influences nuclear localization of viral nucleic acids in nondividing host cells. Proc. Natl Acad. Sci. USA, 91, 7311-7315.
20. Henderson, B. R. & Percipalle, P. (1997). Interactions between HIV Rev and nuclear import and export factors: The Rev nuclear localization signal mediates specific binding to human importin-β. J. Mol. Biol. 274, 693-707.
21. Henkel, T., Zabel, U., Zee, K. V., Muller, J. M., Fanning, E. & baeuerle, P. A. (1992). Intramolecular masking of the nuclear location signal and dimerization domain in the precursor for the p50 NF-κB subunit. Cell, 63, 1121-1133.
22. Huvent, I., Hong, S. S., Fournier, C., Gay, B., Tournier, J., Carriere, C., Courcoul, M., Vigne, R., Spire, B. & Boulanger, P. (1998). Interaction and co-encapsidation of human immunodeficiency virus type 1 Gag and Vif recombinant proteins. J. Gen. Virol. 79, (5), 1069-1081.
23. Karni, O., Friedler, A., Zakai, N., Gilon, C. & Loyter, A. (1998). A peptide derived from the N-terminal region of HIV-1 Vpr promotes nuclear import in permeabilized cells: Elucidation of the NLS region of the Vpr. FEBS Letters, 429, 421-425.
24. Lanford, R. E., White, R. G., Dunham, R. G. & Kanda, P. (1988). Effect of basic and nonbasic amino acid substitutions on transport induced by simian virus 40 T-antigen synthetic peptide nuclear transport signals. Mol. Cell. Biol. 8, (7), 2722-2729.
25. Liu, H., Wu, X., Newman, M., Shaw, G., Hahn, B. & Kappes, J. (1995). The vif protein of human and simian immunodeficiency viruses is packaged into virions and associates with viral core structures. J. Virol. 69, 7630-7638.
26. Melchior, F. & Gerace, L. (1995). Mechanisms of nuclear protein import. Curr. Opin. Cell. Biol. 7, 310-318.
27. Melchior, F., Paschal, B., Evance, J. & Gerace, L. (1993). Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell. Biol. 123, (6), 1649-1659.
28. Miller, R. H. & Sarver, N. (1997). HIV accessory proeins as therapeutic targets. Nature Med. 3, 389-394.
29. Nigg, E. A. (1997). Nucleocytoplasmic transport: Signals, mechanisms and regulation. Nature, 386, 779-787.
30. Picard, D. & Yamamoto, K. R. (1987). Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor. EMBO J. 6, 3333-3340.
31. Popov, S., Rexach, M., Zybarth, G., Reiling, N., Lee, M. A., Ratner, L., Lane, C. M., Moore, M. S., Blobel, G. & Bukrinsky, M. (1998). Viral protein R regulates nuclear import of the HIV-1 pre-integration complex. EMBO J. 17, 909-917.
32. Ruben, S., Perkins, A., Purcell, R., Joung, K., Sia, R., Burghoff, R., Haseltine, W. A. & Rosen, C. A. (1989). Structural and functional characterization of human immunodeficiency virus Tat protein. J. Virol. 63, (1), 1-8.
33. Simm, M., Shahabuddin, M., Chao, W., Allan, J. S. & Volsky, D. J. (1995). Aberrant Gag protein composition of human immunodeficiency virus type 1 vif mutants produced in lymphocytes. J. Virol. 69, 4582-4586.
34. Siomi, H., Shida, H., Maki, M. & Hatanaka, M. (1990). Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization. J. Virol. 64, (4), 1803-1807.
35. Truant, R. & Cullen, B. R. (1999). The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin beta-dependent nuclear localization signals. Mol. Cell. Biol. 19, (2), 1210-1217.
36. Von-Schwedler, U., Song, J., Aiken, C. & Trono, D. (1993). Vif is crucial for HIV-1 proviral DNA synthesis in infected cells. J. Virol. 67, 4945-4955.