Probing the acyl binding site of acetylcholinesterase by protein engineering

Journal of Molecular Catalysis - B Enzymatic

Volumn 6, Issue 3, 1999, Pages 287-296

  Pleiss, Jürgen ^a   Mionetto, Nathalie ^a   Schmid, Rolf D ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Acetylcholinesterase; Inhibitors; Mutation; Protein engineering

Indexed keywords

CATALYST ACTIVITY; HYDROLYSIS; PHOSPHATES; SUBSTRATES; SULFUR COMPOUNDS;

ACETYLCHOLINESTERASE; MUTATION; PROTEIN ENGINEERING;

ENZYMES;

ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE; BUTYRYLTHIOCHOLINE; CARBAMIC ACID; LEUCINE; RECOMBINANT ENZYME;

ACETYLCHOLINE RELEASE; ARTICLE; BINDING SITE; CATALYSIS; ENZYME CONFORMATION; ENZYME SUBSTRATE; GENETIC ENGINEERING; HYDROLYSIS; NONHUMAN; RAT; SITE DIRECTED MUTAGENESIS;

EID: 0033545548     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(98)00082-4     Document Type: Article

References (40)

1. T.L. Rosenberry, in: A. Meister (Ed.), Advances in Enzymology and Related Areas of Molecular Biology, vol. 43, New York, 1975, 103 pp.
2. Massoulié J., Pezzementi L., Bon S., Krejci E., Vallette F.-M. Prog. Neurobiol. 41:1993;31.
3. Schelhaas M., Glomsda S., Haensler M., Jakubke H.-D., Waldmann H. Angew. Chem. 35:1996;106.
4. A. Moretto, M.K. Johnson, in: L.G. Costa (Ed.), Toxicology of Pesticides: Experimental, Clinical and Regulatory Aspects, Springer-Verlag, Berlin H13, 1987, 33 pp.
5. Hart G.H., O'Brien R.D. Biochemistry. 12:1973;2940.
6. L. Horwath, in: VCH (Ed.), Drug and Pesticides, Method in Enzymatic Analysis, vol. 13, 1986, 406 pp.
7. Mionetto N., Marty J.-L., Karube I. Biosens. Bioelectron. 9:1994;463.
8. DIN 38415, 1993, D55-D70.
9. Watts P., Wilkinson R.G. Biochem. Pharmacol. 26:1977;757.
10. Herzsprung P., Weil L., Quentin K.-E. Z. Wasser Abwasser-Forsch. 22:1989;67.
11. Sussman J.L., Harel M., Frolow F., Oefner C., Goldman A., Toker L., Silman I. Science. 253:1991;872.
12. Bourne Y., Taylor P., Marchot P. Cell. 83:1995;503.
13. Harel M., Quinn D.M., Haridasan K.N., Silman I., Sussman J.L. J. Am. Chem. Soc. 118:1996;2340.
14. Ollis D.L., Cheah E., Cygler M., Dijkstra B., Frolow F., Franken S.M., Harel M., Remington S.J., Silman I., Schrag J. Protein Eng. 5:1992;197.
15. Derewenda U., Brzozowski A.M., Lawson D.M., Derewenda Z.S. Biochemistry. 31:1992;1532.
16. Cygler M., Grochulski P., Kazlauskas R.J., Schrag J.D. J. Am. Chem. Soc. 116:1994;3180.
17. Uppenberg J., Ohrner N., Norin M., Hult K., Kleywegt G.J., Patkar S., Waagen V., Anthonsen T., Jones T.A. Biochemistry. 34:1995;16838.
18. van Tilbeurgh H., Egloff M.P., Martinez C., Rugani N., Verger R., Cambillau C. Nature. 362:1993;814.
19. Steinmetz A.C., Demuth H.U., Ringe D. Biochemistry. 33:1994;10535.
20. Mutero A., Pralavorio M., Bride J.M., Fournier D. Proc. Natl. Acad. Sci. U.S.A. 91:1994;5922.
21. Harel M., Sussman J.L., Krejci E., Bon S., Chanal P., Massoulié J., Silman I. Proc. Natl. Acad. Sci. U.S.A. 89:1992;10827.
22. Ordentlich A., Barak D., Kronman C., Flashner Y., Leitner M., Segall Y., Ariel N., Cohen S., Velan B., Shafferman A. J. Biol. Chem. 268:1993;17083.
23. Vellom D.C., Radic Z., Li Y., Pickering N.A., Camp S., Taylor P. Biochemistry. 32:1993;12.
24. Radic Z., Pickering N.A., Vellom D.C., Camp S., Taylor P. Biochemistry. 32:1993;12074.
25. Barak D., Kronman C., Ordentlich A., Ariel N., Bromberg A., Marcus D., Lazar A., Velan B., Shafferman A. J. Biol. Chem. 269:1994;6296.
26. Gnatt A., Loewenstein Y., Yaron A., Schwarz M., Soreq H. J. Neurochem. 62:1994;749.
27. Hosea N.A., Radic Z., Tsigelny I., Berman H.A., Quinn D.M., Taylor P. Biochemistry. 35:1996;10995.
28. Cousin X., Hotelier T., Giles K., Lievin P., Toutant J.-P., Chatonnet A. Nucleic Acids Res. 25:1997;143.
29. Mionetto N., Morel N., Massoulié J., Schmid R.D. Biotechnol. Techniques. 11:1997;805.
30. Mikaelian I., Sergeant A. Nucleic Acids Res. 20:1992;376.
31. Ellman G.L., Courney K.D., Nadres V., Featherstone M. Biochem. Pharmacol. 7:1961;88.
32. Legay C., Bon S., Massoulié J. FEBS Lett. 315:1993;163.
33. Fournier D., Karch F., Bride J.-M., Hall L.M.C., Bergé J.-B., Spierer P. J. Biol. Mol. 210:1989;15.
34. Bairoch A., Boeckmann B. Nucleic Acids Res. 19:1991;2247.
35. Bernstein F.C., Koetzle T.F., Williams G.J., Meyer E.E., Brice M.D., Rodgers J.R., Kennard O., Shimanouchi T., Tasumi M. J. Mol. Biol. 112:1977;535.
36. Thompson J.D., Higgins D.G., Gibson T.J. Nucleic Acids Res. 22:1994;4673.
37. Depiereux E., Feytmans E. Protein Eng. 4:1991;603.
38. Kabsch W., Sander C. Biopolymers. 22:1983;2577.
39. Mutero A., Pralavorio M., Simeon V., Fournier D. NeuroReport. 3:1992;39.
40. Hosea N.A., Berman H.A., Taylor P. Biochemistry. 34:1995;11528.