The effect of counterion, water concentration, and stirring on the stability of subtilisin BPN' in organic solvents

Journal of Molecular Catalysis - B Enzymatic

Volumn 6, Issue 3, 1999, Pages 297-304

  Sears, Pamela ^a   Witte, Krista ^a   Wong, C H ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Counterion; Subtilisin BPN'; Water concentration

Indexed keywords

COMPOSITION EFFECTS; DRYING; IONS; ORGANIC SOLVENTS; SALTS; SODIUM COMPOUNDS; SOLUBILITY; WATER;

COUNTERIONS; DIMETHYLFORMAMIDE; SODIUM CITRATE; SUBTILISIN;

ENZYMES;

CITRATE SODIUM; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; SODIUM DIHYDROGEN PHOSPHATE; SUBTILISIN;

ARTICLE; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME STABILITY; FREEZE DRYING; SOLUBILITY;

EID: 0033545522     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(98)00085-X     Document Type: Article

References (26)

1. Homandberg G.A., Mattis J.A., Laskowski M. Biochemistry. 17:1978;5220-5227.
2. Schellenberger V., Jakubke H.-D. Angew. Chem., Int. Ed. Engl. 30:1991;1437-1449.
3. Witte K.L., Sears P., Martin R., Wong C.-H. J. Am. Chem. Soc. 119:1997;2114-2118.
4. Wong C.-H., Schuster M., Wang P., Sears P. J. Am. Chem. Soc. 115:1993;5893-5901.
5. Riva S., Chopineau J., Kieboom A.P.G., Klibanov A.M. J. Am. Chem. Soc. 110:1988;584-589.
6. Hennen W.J., Sweeres H.M., Wang Y.-F., Wong C.-H. J. Org. Chem. 53:1988;4939-4945.
7. Effenberger F., Graef B.W., Osswald S. Tetrahedron Asymmetry. 8:1997;2749-2755.
8. Chen S.-C., Wu S.-H., Girdaukas G., Sih C.J. J. Am. Chem. Soc. 109:1987;2812-2817.
9. Jones J.B., Beck J.F. Tech. Chem. (N.Y.). 10:1976;107-401.
10. Chen C.S., Sih C.J. Angew. Chem. 101:1989;711-724.
11. Park H.G., Chang H.N., Dordick J.S. Biotechnol. Lett. 17:1995;1085-1090.
12. Gunata Z., Vallier M.J., Sapis J.C., Baumes R., Bayonove C. Enzyme Microb. Technol. 16:1994;1055-1058.
13. Finch P., Yoon J.H. Carbohydr. Res. 303:1997;339-345.
14. Chen K., Arnold F.H. Biotechnology. 9:1991;1073-1076.
15. Sears P., Wong C.-H. Biotechnol. Prog. 12:1996;423-433.
16. Khmelnitsky Y.L., Welch S.H., Clark D.S., Dordick J.S. J. Am. Chem. Soc. 116:1994;2647-2648.
17. Dabulis K., Klibanov A.M. Biotechnol. Bioeng. 41:1993;566-571.
18. Dordick J.S. Enzyme Microb. Technol. 11:1989;194-211.
19. Affleck R., Xu X.-F., Suzawa V., Focht K., Clark D.S., Dordick J.S. Proc. Natl. Acad. Sci. U.S.A. 89:1992;1100-1104.
20. Sears P., Schuster M., Wang P., Witte K.L., Wong C.-H. J. Am. Chem. Soc. 116:1994;6521-6530.
21. Zacharis E., Omar I.C., Partridge J., Robb D.A., Halling P.J. Biotechnol. Bioeng. 55:1997;367-374.
22. Zaks A., Klibanov A.M. Biochemistry. 82:1985;3192-3196.
23. Yang Y., Zacherl D., Russell A.J. J. Am. Chem. Soc. 115:1993;12251-12257.
24. Shubhada S., Sundaram P.V. Enzyme Microb. Technol. 17:1995;330-335.
25. Kim M.G., Lee S.B. J. Mol. Catal. B. 2:1996;127-140.
26. Wescott C.R., Klibanov A.M. J. Am. Chem. Soc. 115:1993;1629.