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Volumn 285, Issue 5425, 1999, Pages 251-254

Ploidy regulation of gene expression

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CYCLE; GENE EXPRESSION REGULATION; GENE INDUCTION; GENE REPRESSION; HAPLOIDY; NONHUMAN; PLOIDY; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; TETRAPLOIDY;

EID: 0033538465     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5425.251     Document Type: Article
Times cited : (533)

References (34)
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    • Isogenic strains were derived from Saccharomyces cerevisiae ∑1278b MATa ura3-52 leu2::hisG his3::hisG. Mating types were switched (for example, MATa to MATα, and MATa/α to MATa/a) by transient expression of the HO endonuclease from pGAL-HO. A brief exposure to galactose was followed by curing of pGAL-HO. Mating types were confirmed through mating and pheromone production assays. Matings produced the desired ploidy (for example, 2n MATa/a × 2n MATα/α → 4n MATa/a/α/α). Ploidies of these zygotes were confirmed by tetrad dissections and analysis of mating-type segregation.
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    • Expression data for each gene, as well as each reference pattern, were normalized to have mean = 0 and SD = 1. Pearson correlation coefficients were then calculated for each gene against a reference pattern either proportional to ploidy (haploids = 1, diploids = 2, and so forth) or inversely proportional (haploids = 1, diploids = 1/2, and so forth). The first criterion was that the correlation exceeded a threshold (Fig. 1). The second criterion was that the maximum and minimum average difference values for a gene differed by at least 100 units and at least a factor of 3. Ten random data sets (within-gene permutations of the experimental data set) showed that these criteria resulted in less than one false positive on average.
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    • note
    • We thank G. Acton, A. Dranginis, Q. Feng, T. Golub, M. Gordon, P. Hecht, F. Holstege, E. Jennings, F. Lewitter, H. Madhani, T. Ni, T. Orr-Weaver, J. Park, S. Rozen, D. Slonim, P. Tamayo, N. Watson, and R. Young for their contributions. Supported by Bristol-Meyers Squibb Company, Affymetrix Inc., and Millennium Pharmaceutical Inc., and by NIH grant GM35010. G.R.F. is an American Cancer Society Professor of Genetics. T.G. is a postdoctoral fellow of the Helen Hay Whitney Foundation.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.