Biosynthesis of isoprenoids in Escherichia coli: stereochemistry of the reaction catalyzed by farnesyl diphosphate synthase

Organic Letters

Volumn 1, Issue 7, 1999, Pages 1071-1073

  Leyes, Aquiles E ^a   Baker, Jonathan A ^a   Poulter, C Dale ^a  

^a UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3,3 DIMETHYLALLYL PYROPHOSPHATE; 3,3-DIMETHYLALLYL PYROPHOSPHATE; GERANYLTRANSFERASE; HEMITERPENE; ISOPENTENYL DIPHOSPHATE; ORGANOPHOSPHORUS COMPOUND; TRANSFERASE;

ANIMAL; ARTICLE; CATALYSIS; CHICKEN; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM; STEREOISOMERISM; SWINE;

ALKYL AND ARYL TRANSFERASES; ANIMALS; CATALYSIS; CHICKENS; ESCHERICHIA COLI; GERANYLTRANSTRANSFERASE; HEMITERPENES; ORGANOPHOSPHORUS COMPOUNDS; STEREOISOMERISM; SWINE;

EID: 0033533636     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol990876e     Document Type: Article

References (20)

1. Abbreviations used: BHDA = bicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid; DMAPP = dimethylallyl diphosphate; FPP = farnesyl diphosphate; GPP = geranyl diphosphate; IPP = isopentenyl diphosphate; IPTG = isopropyl-β-D-galactopyranoside; MEP = methylerythrose phosphate; TPP = thiamine diphosphate.
2. For a review, see: Poulter, C. D.; Rilling, H. C. In Biosynthesis of Isoprenoid Compounds: Porter, J. W.; Porter, S. L., Eds.; Wiley: New York, 1981.
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9. One unit is the amount of protein (mg) that can produce 1 μmol of product in 1 min.
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11. 3 for 5 min and then linear gradient to 100% MeCN in 25 min.
12. The MEP nomenclature is parallel to the MVA (mevalonate) pathway.
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17. All vinylic positions derived from C2 of IPP.
18. Arigoni, D.; Eisenreich, W.; Latzel, C.; Sagner, S.; Radykewicz, T.; Zenk, M. H.; Bacher, A. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 1309.
19. Hahn, F. M.; Baker, J. A.; Poulter, C. D. J. Bacteriol. 1996, 178, 619. The cloning and characterization of the E. coli isomerase has been reported: Hahn, F. M.; Hurlburt, A. L.; Poulter, C. D. J. Bacteriol. 1999, in press.
20. Hahn, F. M.; Baker, J. A.; Poulter, C. D. J. Bacteriol. 1996, 178, 619. The cloning and characterization of the E. coli isomerase has been reported: Hahn, F. M.; Hurlburt, A. L.; Poulter, C. D. J. Bacteriol. 1999, in press.