A targeted deletion of a region upstream from the J(κ) cluster impairs κ chain rearrangement in cis in mice and in the 103/bcl2 cell line

Journal of Experimental Medicine

Volumn 189, Issue 9, 1999, Pages 1443-1449

  Cocea, Laurentiu ^a   De Smet, Annie ^a   Saghatchian, Mahasti ^a,c   Fillatreau, Simon ^a,d   Ferradini, Laurent ^a   Schurmans, Stéphane ^b   Weill, Jean Claude ^a   Reynaud, Claude Agnès ^a  

^a INSERM   (France)

^b UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^c INSTITUT GUSTAVE ROUSSY   (France)

^d UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Allelic exclusion; Germline promoter; Mouse immunoglobulin genes; Positive regulatory element; Regulation of rearrangement

Indexed keywords

IMMUNOGLOBULIN KAPPA CHAIN; IMMUNOGLOBULIN LIGHT CHAIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EMBRYO; GENE REARRANGEMENT; GENE TARGETING; IMMUNOGLOBULIN GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION;

ALLELES; ANIMALS; B-LYMPHOCYTES; CELL LINE; GENE REARRANGEMENT, B-LYMPHOCYTE; GENE TARGETING; GERM CELLS; IMMUNOGLOBULIN JOINING REGION; IMMUNOGLOBULIN KAPPA-CHAINS; MICE; MUTAGENESIS; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

EID: 0033519234     PISSN: 00221007     EISSN: None     Source Type: Journal    
DOI: 10.1084/jem.189.9.1443     Document Type: Article

References (28)

1. Sleckman, B.P., J.R. Gorman, and F.W. Alt. 1996. Accessibility control of antigen-receptor variable-region gene assembly: role of cis-acting elements. Annu. Rev. Immunol. 14:459 481.
2. Chen, J., and F.W. Alt. 1993. Gene rearrangement and B-cell development. Curr. Opin. Immunol. 5:194-200.
3. Schlissel, M.S., and P. Stanhope-Baker. 1997. Accessibility and the developmental regulation of V(D)J recombination. Semin. Immunol. 9:161-170.
4. Villev, I., D. Caillol, F. Selz, P. Ferrier, and J.P. de Villartay. 1996. Defect in rearrangement of the most 5′ TCR-J alpha following targeted deletion of T early alpha (TEA): implications for TCR alpha locus accessibility. Immunity. 5:331-342.
5. Kallenbach, S., C. Babinet, S. Pournin, P. Cavelier, M. Goodhardt, and F. Rougeon. 1993. The intronic immunoglobulin kappa gene enhancer acts independently on rearrangement and on transcription. Eur. J. Immunol. 23:1917-1921.
6. Okada, A., M. Mendelsohn, and F.W. Alt. 1994. Differential activation of transcription versus recombination of transgenic T cell receptor β variable region gene segments in B and T lineage cells. J. Exp. Med. 180:261-272.
7. Alvarez, J.D., S.J. Anderson, and D.Y. Loh. 1995. V(D)J recombination and allelic exclusion of a TCR beta-chain minilocus occurs in the absence of a functional promoter. J. Immunol. 155:1191-1202.
8. Fernex, C., M. Capone, and P. Ferrier. 1995. The V(D)J recombinational and transcriptional activities of the immunoglobulin heavy-chain intronic enhancer can be mediated through distinct protein-binding sites in a transgenic substrate. Mol. Cell. Biol. 15:3217-3226.
9. Weaver, D., and D. Baltimore. 1987. B lymphocyte-specific protein binding near an immunoglobulin kappa-chain gene J segment. Proc. Natl. Acad. Sci. USA. 84:1516-1520.
10. Ferradini, L., H. Gu, A. De Smet, K. Rajewsky, C.-A. Reynaud, and J.-C. Weill. 1996. Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster. Science. 271:1416-1420.
11. Van Ness, B.G., M. Weigert, C. Coleclough, E.L. Mather, D.E. Kelley, and R.P. Perry. 1981. Transcription of the unrearranged mouse C kappa locus: sequence of the initiation region and comparison of activity with a rearranged V kappa-C kappa gene. Cell. 27:593-602.
12. Martin, D.J., B.G. Van Ness. 1990. Initiation and processing of two kappa immunoglobulin germ line transcripts in mouse B cells. Mol. Cell. Biol. 10:1950-1958.
13. Chen, Y.Y., L.C. Wang, M.S. Huang, and N. Rosenberg. 1994. An active v-abl protein tyrosine kinase blocks immunoglobulin light-chain gene rearrangement. Genes Dev. 8:688-697.
14. Mansour, S.L., K.P. Thomas, and M.R. Capecchi. 1988. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature. 336:348-352.
15. Bernard, H.U., G. Krammer, and W.G. Rowekamp. 1985. Construction of a fusion gene that confers resistance against hygromycin B to mammalian cells in culture. Exp. Cell Res. 158:237-243.
16. Schlissel, M.S., and D. Baltimore. 1989. Activation of immunoglobulin kappa gene rearrangement correlates with induction of germline kappa gene transcription. Cell. 58:1001-1007.
17. Ramsden, D.A., and M. Gellert. 1995. Formation and resolution of double-strand break intermediates in V(D)J rearrangement. Genes Dev. 9:2409-2420.
18. Tian, J., T. Okabe, T. Miyazaki, S. Takeshiba, and A. Kudo. 1997. Pax-5 is identical to EBB-1/KLP and binds to the VpreB and λ5 promoters as well as the KI and KII sites upstream of the JK genes. Eur. J. Immunol. 27:750-755.
19. Madisen, L., and M. Groudine. 1994. Identification of a locus control region in the immunoglobulin heavy-chain locus that deregulates c-myc expression in plasmacytoma and Burkitt's lymphoma cells. Genes Dev. 15:2212-2226.
20. Michaelson, J.S., M. Singh, and B.K. Birshtein. 1996. B cell lineage-specific activator protein (BSAP). A player at multiple stages of B cell development. J Immunol. 156:2349-2351.
21. Fitzsimmons, D., W. Hodsdon, W. Wheat, S.M. Maira. B. Wasylyk, and J. Hagman. 1996. Pax-5 (BSAP) recruits Ets proto-oncogene family proteins to form functional ternary complexes on a B-cell-specific promoter. Genes Dev. 10: 2198-2211.
22. Lauster, R., C.-A. Reynaud, I.-L. Mårtensson, A. Peter, D. Bucchini, J. Jami, and J.-C. Weill. 1993. Promoter, enhancer and silencer elements regulate rearrangement of an immunoglobulin transgene. EMBO (Eur. Mol. Biol. Organ.) J. 12: 4615-4623.
23. Cavelier, P., F. Nato, I. Coquilleau, A. Rolink, F. Rougeon, and M. Goodhardt. 1997. B lineage-restricted rearrangement of a human Ig kappa transgene. Eur. J. Immunol. 27:1626-1631.
24. Cocea, L., A. Dahan, L. Ferradini, C.-A. Reynaud, and J.-C. Weill. 1998. Negative regulation of Ig gene rearrangement by a 150-bp transcriptional silencer. Eur. J. Immunol. 28: 2809-2816.
25. Clausell, A., and P.W. Tucker. 1994. Functional analysis of the V gamma 3 promoter of the murine gamma delta T-cell receptor. Mol. Cell. Biol. 14:803-814.
26. Baker, J.E., D. Cado, and D.H. Raulet. 1998. Developmentally programmed rearrangement of T cell receptor Vgamma genes is controlled by sequences immediately upstream of the Vgamma genes. Immunity. 9:159-168.
27. Mostoslavsky, R., N. Singh, A. Kirillov, R. Pelanda, H. Cedar, A. Chess, and Y. Bergman. 1998. Kappa chain monoallelic demethylation and the establishment of allelic exclusion. Genes Dev. 12:1801-1811.
28. + thymocyte differentiation. J. Immunol. 160: 1256-1267.