Comprehensive restriction analysis of the UL97 region allows early detection of ganciclovir-resistant human cytomegalovirus in an immunocompromised child

Journal of Infectious Diseases

Volumn 180, Issue 2, 1999, Pages 491-495

  Prix, Lothar ^a   Hamprecht, Klaus ^a,c   Holzhüter, Birgit ^b   Handgretinger, Rupert ^b   Klingebiel, Thomas ^b   Jahn, Gerhard ^a  

^a Dept of Med Virol and E ^*  (Germany)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FOSCARNET; GANCICLOVIR; VIRUS DNA;

AGAMMAGLOBULINEMIA; ARTICLE; BONE MARROW TRANSPLANTATION; CASE REPORT; CHILDHOOD DISEASE; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; EARLY DIAGNOSIS; GENE MUTATION; GENOTYPE; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION MAPPING; SCHOOL CHILD; T LYMPHOCYTE;

ANTIVIRAL AGENTS; BONE MARROW TRANSPLANTATION; CHILD, PRESCHOOL; CODON; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; DRUG RESISTANCE, MICROBIAL; GANCICLOVIR; HUMANS; IMMUNOCOMPROMISED HOST; MALE; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RESTRICTION MAPPING; SEQUENCE ANALYSIS, DNA;

EID: 0033508301     PISSN: 00221899     EISSN: None     Source Type: Journal    
DOI: 10.1086/314877     Document Type: Article

References (15)

1. 1 Jabs DA, Enger C, Dunn JP, Forman M. Cytomegalovirus retinitis and viral resistance: ganciclovir resistance. J Infect Dis 1998; 177:770-3.
2. 2. Wolf DG, Yaniv I, Honigman A, Kassis I, Schonfeld T, Ashkenazi S. Early emergence of ganciclovir-resistant human cytomegalovirus strains in children with primary combined immunodeficiency. J Infect Dis 1998; 178: 535-8.
3. 3. Hanson MN, Preheim LC, Chou S, Talarico CL, Biron KK, Erice A. Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir Antimicrob Agents Chemother 1995; 39:1204-5.
4. 4. Chou S, Erice A, Jordan MC, et al. Analysis of the UL97 phosphotransferase coding sequence in clinical cytomegalovirus isolates and identification of mutations conferring ganciclovir resistance, J Infect Dis 1995; 171:576-83.
5. 5. Chou S, Marousek G, Guentzel S, et al. Evolution of mutations conferring multidrug resistance during prophylaxis und therapy of cytomegalovirus disease. J Infect Dis 1997; 176:786-9.
6. 6. Chou S, Guentzel S, Michels KR, Miner RC, Drew WL. Frequency of UL97 phosphotransferase mutations related to ganciclovir resistance in clinical cytomegalovirus isolates. J Infect Dis 1995; 172:239-42.
7. 7. Hamprecht K, Mikeler M, Jahn G. Semi-quantitative detection of cytomegalovirus DNA from native serum and plasma by nested PCR: influence of DNA extraction procedures. J Virol Methods 1997; 69:125-35.
8. 8. Prix L, Maierl J, Jahn G, Hamprecht K. A simplified assay for screening of drug resistance of cell-associated cytomegalovirus strains. J Clin Virol 1998; 11:29-37.
9. 9. Prix L, Kuner R, Speer CP, Jahn G, Hamprecht K. Evaluation of restriction fragment length polymorphism analysis of the UL10-UL13 genomic region for rapid identification of human cytomegalovirus strains. Eur J Clin Microbiol Infect Dis 1998; 17:525-8.
10. 10. Zaia JA, Gallez-Hawkins G, Churchill MA, et al. Comparative analysis of human cytomegalovirus α-sequence in multiple clinical isolates by using polymerase chain reaction and restriction fragment length polymorphism assay. J Clin Microbiol 1990; 28:2602-7.
11. 11. Chou S, Marousek G, Parenti DM, et al. Mutation in region III of the DNA polymerase gene conferring foscarnet resistance in cytomegalovirus isolates from 3 subjects receiving prolonged antiviral therapy. J Infect Dis 1998; 178:526-30.
12. 12. Erice A, Gil-Roda C, Perez J, et al. Antiviral susceptibilities und analysis of UL97 and DNA polymerase sequences of clinical cytomegalovirus isolates from immunocompromised patients. J Infect Dis 1997; 175:1087-92.
13. 13. Smith IL, Cherrington JM, Jiles RE, Fuller MD, Freeman WR, Spector SA. High-level resistance of cytomegalovirus to ganciclovir is associated with alterations in both the UL97 and DNA polymerase gene. J Infect Dis 1997; 176:69-77.
14. 14. Smith IL, Taskintuna I, Rahal FM, et al. Clinical failure of CMV retinitis with intravitreal cidofovir is associated with antiviral resistance. Arch Ophthalmol 1998; 116:178-85.
15. 17^15. Rosen MR, Benner KG, Flora KD, et al. Development of ganciclovir resistance during treatment of primary cytomegalovirus infection after liver transplantation. Transplantation 1997; 63:476-8.