Phenotypic and genotypic characterization of spotted fever group rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks

Journal of Clinical Microbiology

Volumn 34, Issue 11, 1996, Pages 2688-2694

  Beati, L ^a   Roux, V ^a   Ortuno A ^b   Castella, J ^b   Segura Porta, F ^c   Raoult, D ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c Prog Asistencial Enferm I   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM ISOLATION; BOUTONNEUSE FEVER; DISEASE CARRIER; GENOTYPE; HEMOLYMPH; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RICKETTSIA; TICK BORNE DISEASE;

ACARI; BACTERIA (MICROORGANISMS); RHIPICEPHALUS BURSA; RHIPICEPHALUS SANGUINEUS; RHIPICEPHALUS TURANICUS; RICKETTSIA; RICKETTSIA MASSILIAE;

EID: 0029974094     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.11.2688-2694.1996     Document Type: Article

References (51)

1. Babalis, T., Y. Tselentis, V. Roux, A. Psaroulaki, and D. Raoult. 1994. Isolation and identification of a rickettsial strain related to Rickettsia massiliae in Greek ticks. Am. J. Trop. Med. Hyg. 50:365-372.
2. Bacellar, F. Unpublished data.
3. Bacellar, F., L. R. Regnery, M. S. Núncio, and A. R. Filipe. 1995. Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal. Epidemiol. Infect. 114:169-178.
4. Beati, L., J.-P. Finidori, B. Gilot, and D. Raoult. 1992. Comparison of serologic typing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis, and genetic restriction fragment length polymorphism analysis for identification of rickettsiae: characterization of two new rickettsial strains. J. Clin. Microbiol. 30:1922-1930.
5. Beati, L., J.-P. Finidori, and D. Raoult. 1993. First isolation of Rickettsia slovaca from Dermacentor marginatus in France. Am. J. Trop. Med. Hyg. 48:257-268.
6. Beati, L., P.-F. Humair, A. Aeschlimann, and D. Raoult. 1994. Identification of spotted fever group rickettsiae isolated from Dermacentor marginatus and Ixodes ricinus ticks collected in Switzerland. Am. J. Trop. Med. Hyg. 51:138-148.
7. Beati, L., O. Péter, W. Burgdorfer, A. Aeschlimann, and D. Raoult. 1993. Confirmation that Rickettsia helvetica sp. nov. is a distinct species of the spotted fever group of rickettsiae. Int. J. Syst. Bacteriol. 43:521-526.
8. Beati, L., and D. Raoult. 1993. Rickettsia massiliae sp. nov., a new spotted lever group rickettsia. Int. J. Syst. Bacteriol. 43:839-840.
9. Bella, F., E. Espero, S. Uriz, J. A. Serrano, M. A. Allegre, and J. Tort. 1991. Randomized trial of 5-day rifampin versus 1-day doxycycline therapy for MSF. J. Infect. Dis. 164:433-434.
10. Breitschwerdt, E. B., D. H. Walker, M. G. Levy, W. Burgdorfer, W. T. Corbett, S. A. Hurlbert, M. E. Stebbins, B. C. Curtis, and D. A. Allen. 1988. Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana. Am. J. Vet. Res. 49:70-76.
11. Burgdorfer, W. 1970. Hemolymph test. A technique for detection of rickettsiae in ticks. Am. J. Trop. Med. Hyg. 19:1010-1014.
12. Davis, G. E., and H. R. Cox. 1938. A filter-passing infectious agent isolated from ticks. I. Isolation from D. andersoni, reactions in animals and filtration experiments. Public Health Rep. 53:2259-2267.
13. Eremeeva, M., X. Yu, and D. Raoult. 1994. Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA. J. Clin. Microbiol. 32:803-810.
14. Eremeeva, M. E., N. M. Balayeva, and D. Raoult. 1994. Purification of rickettsial cultures contaminated by mycoplasmas. Acta Virol. 38:231-233.
15. Eremeeva, M. E., L. Beati, V. A. Makarova, N. F. Fetisova, I. V. Tarasevich, N. M. Balayeva, and D. Raoult. 1994. Astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and Rhipicephalus pumilio ticks. Am. J. Trop. Med. Hyg. 51:697-706.
16. Espejo, E., M. D. Alegre, B. Font, A. Font, F. Segura, and F. Bella. 1993. Antibodies to Rickettsia conorii in dogs: seasonal differences. Eur. J. Epidemiol. 9:344-346.
17. Espejo-Arenas, E., B. Font-Creus, M.D. Alegre-Segura, F. Segura-Porta, and F. Bella-Cueto. 1990. Seroepidemiological survey of Mediterranean spotted fever in an endemic area ('Vallés occidental,' Barcelona, Spain). Trop. Geogr. Med. 42:212-216.
18. Espejo-Arenas, E., and D. Raoult. 1989. First isolates of Rickettsia conorii in Spain using a centrifugation-shell vial assay. J. Infect. Dis. 159:1158-1159.
19. Feldman-Muhsam, B. 1953. Rhipicephalus bursa in Israel. Bull. Res. Council Israel 3:201-206.
20. Font-Creus, B., F. Bella-Cueto, E. Espejo-Arenas, R. Vidal-Sanahuja, T. Muñoz-Espin, M. Nolla-Salas, A. Casagran-Borrell, J. Mercade-Cuesta, and F. Segura-Porta. 1985. Mediterranean spotted fever: a cooperative study of 227 cases. Rev. Infect. Dis. 7:635-642.
21. Font-Creus, B., E. Espejo-Arenas, T. Muñoz-Espín, S. Uriz-Urzainqui, F. Bella-Cueto, and F. Segura-Porta. 1991. Fiebre botonosa mediterránea. Estudio de 246 casos. Med. Clin. 96:121-125.
22. Giménez, D. F. 1964. Staining rickettsiae in yolk-sac cultures. Stain Technol. 39:135-140.
23. Herrero, C., C. Pelaz, J. Alvar, R. Molina, J. Vázquez, P. Anda, J. Casal, and C. Martin-Bourgon. 1992. Evidence of the presence of spotted fever group rickettsiae in clogs and dog ticks of central provinces in Spain. Eur. J. Epidemiol. 8:575-579.
24. Herrero, C., C. Pelaz, and C. Martin-Bourgon. 1992. Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain. Epidemiol. Infect. 108:555-557.
25. Herrero-Herrero, J. I., R. Ruiz-Beltrán, and A. M. Martín-Sánchez. 1991. Naturally acquired immunity: a means of resistance to Mediterranean spotted fever? J. Infect. Dis. 154:618-619.
26. Herrero-Herrero, J. I., R. Ruiz-Beltrán, A. M. Martín-Sánchez, and E. J. Garcia. 1989. Mediterranean spotted fever in Salamanca, Spain. Epidemiological study in patients and serosurvey in animals and healthy human population. Acta Trop. 46:335-350.
27. Herrero-Herrero, J. I., R. Ruiz-Beltran, and D. H. Walker. 1993. Antigens of Rickettsia canorii recognized by seropositive healthy people from Salamanca (Central-West Spain). Eur. J. Epidemiol. 9:59-63.
28. Kelly, P. J., L. Beati, P. R. Mason, L. A. Matthewman, V. Roux, and D. Raoult. 1996. Rickettsia africae sp. nov., the etiological agent of African tick bite fever. Int. J. Syst. Bacteriol. 46:611-614.
29. Kelly, P. J., L. Beati, L. A. Matthewman, P. R. Mason, and D. Raoult. 1994. A new pathogenic spotted fever group rickettsia from Africa. J. Trop. Med. Hyg. 97:129-137.
30. Kelly, P. J., D. Raoult, and P. R. Mason. 1991. Isolation of spotted fever group rickettsias from triturated ticks using a modification of the centrifugation-shell vial technique. Trans. R. Soc. Trop. Med. Hyg. 85:397-398.
31. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
32. Largo Aguado, F., J. L. Perez Ortiz, A. Mingo, A. Tutor Martinez, F. Martinez Gongora, and A. Menendez Lozano. 1982. Fiebre botonosa en nuestro medio: estudio clínico prospectivo de 21 casos. Rev. Clin. Esp. 166:51-53.
33. Morel, P. C., and G. Vassiliades. 1962. Les Rhipicephales du groupe sanguineus: espèces africaines. Rev. Elev. Med. Vet. Pays Trop. 15:343-386.
34. Otero, R., A. Fenoll, and J. Casal. 1982. Resurgence of Mediterranean spotted fever. Lancet ii:1107.
35. Péter, O., D. Raoult, and B. Gilot. 1990. Isolation by a sensitive centrifugation cell culture system of 52 strains of spotted fever group rickettsiae from ticks collected in France. J. Clin. Microbiol. 28:1597-1599.
36. Philip, R. N., E. A. Casper, W. Burgdorfer, R. K. Gerloff, L. E. Hughes, and E. J. Bell. 1978. Serologic typing of rickettsiae of the spotted fever group by indirect microimmunofluorescence. J. Immunol. 121:1961-1968.
37. Raoult, D. Unpublished data.
38. Raoult, D., P. Rousselier, G. Vestris, and J. Tamalet. 1987. In vitro antibiotic susceptibility of Rickettsia rickettsii and Rickettsia conorii: plaque assay and microplaque colorimetric assay. J. Infect. Dis. 155:1059-1062.
39. Regnery, R. L., C. L. Spruill, and B. D. Plikaytis. 1991. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J. Bacteriol. 173:1576-1589.
40. Roux, V., M. Drancourt, and D. Raoult. 1992. Determination of genome sizes of Rickettsia spp. within the spotted fever group, using pulsed-field gel electrophoresis. J. Bacteriol. 174:7455-7457.
41. Roux, V., and D. Raoult. 1993. Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis. J. Bacteriol. 175:4895-4904.
42. Roux, V., and D. Raoult. 1995. Phylogenetic analysis of the genus Rickettsia by 16S rDNA sequencing. Res. Microbiol. 146:385-396.
43. Roux, V., E. Rydkina, M. Eremeeva, and D. Raoult. Citrate synthase gene comparison of a new tool for phylogenetic analysis, the Rickettsia paradigm. Submitted for publication.
44. Ruiz, R., J. I. Herrero, A. M. Martin, F. Sanz, A. Mateos, A. Hernandez, R. Querol, and J. Portugal. 1984. Vascular permeability in boutonneuse fever. J. Infect. Dis. 149:1036.
45. Ruiz Beltrán, R., J. I. Herrero Herrero, A. M. Martín Sánchez, V. Vicente García, F. Sanz Ortega, A. Mateos Sánchez, R. Querol Prieto, and J. de Portugal Alvarez. 1985. Formas graves de fiebre exantemática mediterránea. Análisis prospectivo de 71 enfermos. An. Med. Intern. (Madrid) 2:365-368.
46. Ruiz-Beltrán, R., J. I. Herrero-Herrero, D. H. Walker, and A. Cuñado-Rodríguez. 1990. Mechanism of upper gastrointestinal hemorrhage in Mediterranean spotted fever. Trop. Geogr. Med. 42:78-82.
47. Segura, F., and B. Font. 1982. Resurgence of Mediterranean spotted fever in Spain. Lancet ii:280.
48. Segura-Porta, F., B. Font-Creus, E. Espejo-Arenas, and F. Bella-Cueto. 1989. New trends in Mediterranean spotted fever. Eur. J. Epidemiol. 5:438-443.
49. Tatlock, H. 1944. A rickettsia-like organism recovered from guinea pigs. Proc. Soc. Exp. Biol. Med. 57:95-99.
50. Towbin, A., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
51. Weiss, E., J. C. Coolbaugh, and J. C. Williams. 1975. Separation of viable Rickettsia typhi from yolk sac and L cell host components by Renografin density gradient centrifugation. Appl. Microbiol. 30:456-463.