Alkaloid production by a Cinchona officinalis 'Ledgeriana' hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus

Plant Cell Reports

Volumn 19, Issue 2, 1999, Pages 191-196

  Geerlings, A ^a   Hallard, D ^a   Martinez Caballero, A ^a   Lopes Cardoso, I ^a   Van Der Heijden, R ^a   Verpoorte, R ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Catharanthus roseus; Cinchona officinalis 'Ledgeriana'; Genetic engineering; Quinoline alkaloids; Terpenoid indole alkaloids

Indexed keywords

ALKALOID; AROMATIC LEVO AMINO ACID DECARBOXYLASE; BIOSYNTHESIS; COMPLEMENTARY DNA; GENE EXPRESSION; GENE INSERTION; HAIRY ROOT CULTURE; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; REPORTER GENE; SOUTHERN BLOTTING; STRICTOSIDINE SYNTHASE;

CATHARANTHUS ROSEUS; CINCHONA CALISAYA; CINCHONA LEDGERIANA; CINCHONA OFFICINALIS; CINCHONA PUBESCENS;

EID: 0033492452     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990050732     Document Type: Article

References (22)

1. Aerts RJ, Leer T van der, Heijden R van der, Verpoorte R (1990) Developmental regulation of alkaloid production in Cinchona seedlings. J Plant Physiol 136:86-91
2. De Luca V, Marineau C, Brisson N (1989) Molecular cloning and analysis of a cDNA encoding a plant tryptophan decarboxylase: comparison with animal dopa decarboxylases. Proc Natl Acad Sci USA 86:2582-2586
3. Finnegan J, McElroy D (1994) Transgene inactivation: plants fight back. Biotechnology 12:883-888
4. Goddijn OJM, Duyn Schouten PM van der, Schilperoort RA, Hoge JHC (1993) A chimaeric tryptophan decarboxylase gene as a novel selectable marker in plant cells. Plant Mol Biol 22:907-912
5. Giroud C, Leer T van der, Heijden R van der, Verpoorte R, Heeremans CEM, Niessen WMA, Greef J van der (1991) Thermospray liquid chromatography/mass spectrometry (TSP LC/MS) analysis of the alkaloids from Cinchona in vitro cultures. Planta Med 57:142-148
6. Hallard D, Heijden R van der, Lopes Cardoso MI, Pasquali G, Memelink J, Hoge JHC, Verpoorte R (1997) Suspension cultured transgenic cells of Nicotiana tabacum expressing the tryptophan decarboxylase and strictosidine synthase enzymes from Catharanthus roseus produce and release strictosidine upon feeding of secologanin. Plant Cell Rep 17:50-54
7. Hamill JD, Robins RJ, Rhodes MJC (1989) Alkaloid production by transformed root cultures of Cinchona ledgeriana. Planta Med 55:354-357
8. Hoekstra SS (1993) Accumulation of indole alkaloids in plant-organ cultures. PhD thesis, Leiden University
9. McKnight TD, Roessner CA, Devagupta R, Scott AI, Nessler CL (1990) Nucleotide sequence of a cDNA encoding the vacuolar protein strictosidine synthase from Catharanthus roseus. Nucleic Acids Res 18:4939
10. Meijer AH, Verpoorte R, Hoge JHC (1993) Regulation of enzymes and genes involved in terpenoid indole alkaloid biosynthesis in Catharanthus roseus. J Plant Res Special Issue 3:145-164
11. Memelink J, Swords KMM, Staehelin LA, Hoge JHC (1994) Southern, Northern and Western blot analysis. In: Gelvin SB, Schilperoort RA (eds) Plant molecular Biololgy Manual. Kluwer, Dordrecht, pp 1-23
12. Pennings EJM, Hegger I, Heijden R van der, Duine JA, Verpoorte R (1987) Assay of tryptophan decarboxylase from Catharanthus roseus plant cell cultures by high-performance liquid chromatography. Anal Biochem 165:133-136
13. Pennings EJM, Bosch RA van den, Heijden R van der, Stevens LH, Duine JA, Verpoorte R (1989) Assay of strictosidine synthase from plant cell cultures by high performance liquid chromatography. Anal Biochem 176:412-415
14. Peterson GL (1977) Simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83:346-356
15. Poulsen C, Goddijn OJM, Hoge JHC, Verpoorte R (1994) Anthranilate synthase and chorismate mutase activities in transgenic tobacco plants overexpressing tryptophan decarboxylase from Catharanthus roseus. Transgenic Res 3:43-49
16. Skinner NJ, Robins RJ, Rhodes MJC (1987) Tryptophan decarboxylase, strictosidine synthase and alkaloid production by Cinchona ledgeriana cell suspension cultures. Phytochemistry 26:721-725
17. Stevens LH, Schripsema J, Pennings EJM, Verpoorte R (1992) Activities of enzymes involved in indole biosynthesis in suspension cultures of Catharanthus, Cinchona and Tabernaemontana species. Plant Physiol Biochem 30:675-681
18. Stevens LH, Blom TJM, Verpoorte R (1993a) Subcellular localization of trryptophan decarboxylase, strictosidine synthase and strictosidsine β-D-glucosidase in suspension cultured cells of Catharanthus roseus and Tabernaemontana divaricata. Plant Cell Rep. 12:573-576
19. Stevens LH, Giroud C, Pennings EJM, Verpoorte R (1993b) Purification and characterization of strictosidine synthase from a suspension culture of Cinchona robusta. Phytochemistry 33:99-106
20. Verpoorte R, Harkes PAA, Hoopen HJG ten (1987) A biotechmcal production process for Cinchona alkaloids: prospects and problems. Acta Leidensia 55:29-44
21. Verpoorte R, Heijden R van der, Gulik WM van, Hoopen HJG ten (1991) Plant biotechnology for the production of alkaloids: present status and prospects. In: Brossi A (ed) The alkaloids, vol 40. Academic Press, San Diego, pp 1-187
22. Webb DM, Knapp SJ (1990) DNA extraction from a previously recalcitrant plant genus. Plant Mol Biol 8:180-185