Suspension cultured transgenic cells of Nicotiana tabacum expressing tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus produce strictosidine upon secologanin feeding

Plant Cell Reports

Volumn 17, Issue 1, 1997, Pages 50-54

  Hallard, D ^a   Van Der Heijden, R ^a   Verpoorte, R ^a   Lopes Cardoso, M I ^b   Pasquali, G ^b   Memelink, J ^b   Hoge, J H C ^b  

^a LEIDEN UNIVERSITY   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Nicotiana tabacum; Secologanin; Strictosidine synthase; Terpenoid indole alkaloids; Tryptophan decarboxylase

Indexed keywords

NICOTIANA TABACUM;

EID: 0031416208     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990050350     Document Type: Article

References (25)

1. Berlin J, Rügenhagen C, Dietze P, Fecker LF, Goddijn OJM, Hoge JHC (1993) Increased production of serotonin by suspension and root cultures of Peganum harmala transformed with a tryptophan decarboxylase cDNA clone from Catharanthus roseus. Transgenic Res 2:336-344
2. Chavadej S, Brisson N, McNeil JN, De Luca V (1994) Redirection of tryptophan leads to production of low indole glucosinolate canola. Proc Natl Acad Sci USA 91:2166-2170
3. De Luca V, Cutler AJ (1987) Subcellular localization of enzymes involved in indole alkaloid biosynthesis in Catharanthus roseus. Plant Physiol 85:1099-1102
4. Ditta G, Stanfield S, Corbin D, Helinski DR (1980) Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77:7347-7351
5. Goddijn OJM (1992) Regulation of terpenoid indole alkaloid biosynthesis in Catharanthus roseus: the tryptophan decarboxylase gene. PhD thesis, Leiden University
6. Goddijn OJM, Duijn Schouten PM van der, Schilperoort RA, Hoge JHC (1993) A chimaeric tryptophan decarboxylase gene as a novel selectable marker in plant cells. Plant Mol Biol 22:907-912
7. Heijden R van der. Lamping PJ, Out PP, Wijnsma R, Verpoorte R (1987) High-performance liquid Chromatographic determination of indole alkaloids in a suspension culture of Tabernaemontana divaricata. J Chromatogr 396:286-295
8. Horsch RB, Fry JE, Hoffmann NL, Eichhioltz Rogers SG, Fraley RT (1985) A simple and general method of transferring genes into plants. Science 227:1229-1231
9. Kutchan TM, Dittrich H, Bracher D, Zenk MH (1991) Enzymology and molecular biology of alkaloid biosynthesis. Tetrahedron 47:5945-5954
10. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100-127
11. Lopes Cardoso MI (1996) Analysis and modification of the expression of genes involved in plant secondary metabolism: genes encoding early steps in terpenoid indole alkaloid biosynthesis in Catharanthus roseus. PhD thesis, Catholic University of Portugal
12. McKnight TD, Bergey DR, Burnett RJ, Nessler CL (1991) Expression of enzymatically active and correctly targeted strictosidine synthase in transgenic tobacco plants. Planta 185:148-152
13. Moreno PRH, Heijden R van der, Verpoorte R (1993) Effect of terpenoid precursor feeding and elicitation on formation of indole alkaloids in cell suspension cultures of Catharanthus roseus. Plant Cell Rep 12:702-705
14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
15. Nessler CL (1994) Metabolic engineering of plant secondary products. Transgen Res 3:109-115
16. Pasquali G, Goddijn OJM, Waal A de, Verpoorte R, Schilperoort RA, Hoge JHC, Memelink J (1992) Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors. Plant Mol Biol 18:1121-1131
17. Pennings EJM, Hegger I, Heijden R van der, Duine JA, Verpoorte R (1987) Assay of tryptophan decarboxylase from Catharanthus roseus plant cell cultures by high-performance liquid chromatography. Anal Biochem 165:133-136
18. Pennings EJM, Bosch RA van den, Heijden R van der, Stevens LH, Duine JA, Verpoorte R (1989) Assay of strictosidine synthase from plant cell cultures by high-performance liquid chromatography. Anal Biochem 176:412-415
19. Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83:346-356
20. Poulsen C, Goddijn OJM, Hoge JHC, Verpoorte R (1994) Anthranilate synthase and chorismate mutase activities in transgenic tobacco plants overexpressing tryptophan decarboxylase from Catharanthus roseus. Transgen Res 3:43-49
21. Schripsema J, Verpoorte R (1992) Search for factors involved in indole alkaloid production in cell suspension cultures of Tabernaemontana divaricata. Planta Med 58:245-249
22. Songstad DD, De Luca V, Brisson N, Kurz WGW, Nessler CL (1990) High levels of tryptamine accumulation in transgenic tobacco expressing tryptophan decarboxylase. Plant Physiol 94:1410-1413
23. Songstad DD, Kurz WGW, Nessler CL (1991) Tyramine accumulation in Nicotiana tabacum transformed with a chimeric tryptophan decarboxylase gene. Phytochemistry 30:3245-3246
24. Stevens LH, Blom TJM, Verpoorte R (1993) Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine β-D-glucosidase in suspension cultured cells of Catharanthus roseus and Tabernaemontana divaricata. Plant Cell Rep 12:573-576
25. Vancanneyt G, Schmidt R, O'Connor-Sanchez A, Wilmitzer L, Rocha-Sosa M (1990) Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events. Mol Gen Genet 220:245-250