A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis

Nematology

Volumn 1, Issue 6, 1999, Pages 655-660

  Qiu, Lihong ^a   Bedding, Robin ^a  

^a NONE

Author keywords

Energy reserve; Quality control; Steinernema carpocapsae

Indexed keywords

BODY SIZE; COMPUTER ANALYSIS; DRY WEIGHT; ENTOMOPATHOGENIC NEMATODE; LIPID ANALYSIS; PESTICIDE PRODUCTION; QUALITY CONTROL; SIZE;

ENTOMOPATHOGENIC ORGANISM; ESTIMATION METHOD; IMAGE ANALYSIS; LIPID; NEMATODE; WEIGHT;

NEMATODA;

EID: 0033491506     PISSN: 13885545     EISSN: None     Source Type: Journal    
DOI: 10.1163/156854199508612     Document Type: Article

References (7)

1. BEDDING, R.A. (1981). Low cost in vitro mass production of Neoaplectana and Heterorhabditis species (Nematoda) for field control of insect pests. Nematologica 27, 109-114.
2. BEDDING, R.A., STANFIELD, M.S. & CROMPTON, G.W. (1991). Apparatus and Method for Rearing Nematodes, Fungi, Tissue Cultures and The Like, and For Harvesting Nematodes. International Patent Application No PCT/AU91/00136.
3. FITTERS, P.F.L., MEIJER, E.M.J., WRIGHT, D.J. & GRIFFIN, C.T. (1997). Estimation of lipid reserves in unstained living and dead nematodes by image analysis. Journal of Nematology 29, 160-167.
4. GREWAL, P.G. (1995). Nematode quality. In: The Second International symposium on Entomopathogenic Nematodes and their Symbiotic Bacteria. Honolulu, Hawaii, USA, University of Hawaii, pp. 38-40.
5. PATEL, M.N. & WRIGHT, D.J. (1997). Glycogen: its importance in the infectivity of the aged juveniles of Steinernema carpocapsae. Parasitology 114, 591-596.
6. PATEL, M.N., STOLINSKI, M. & WRIGHT, D.J. (1997). Neutral lipids and the assessment of infectivity in entomopathogenic nematodes - observations on four Steinernema species. Parasitology 114, 489-496.
7. QIU, L.H. & BEDDING, R.A. (1999). The relationship between the survival and the energy metabolism of the infective juveniles of Steinernema carpocapsae under both aerobic and anaerobic conditions. In: Survival of Entomopathogenic Nematodes (COST ACTION 819). Wellesbourne, Warwick, UK, pp. 149-159.