Estimation of Lipid Reserves in Unstained Living and Dead Nematodes by Image Analysis

Journal of Nematology

Volumn 29, Issue 2, 1997, Pages 160-167

  Fitters, P F L ^a   Meijer, E M J ^b   Wright, D J ^c   Griffin, C T ^a  

^a ST PATRICK S COLLEGE   (Ireland)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Entomopathogenic nematode; Heterorhabditis; Image analysis; Neutral lipid; Oil Red O; Optical density; Steinernema; Triglyceride

Indexed keywords

EID: 0030850074     PISSN: 0022300X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Alphey, T. J. W. 1982. The effect of host plant on the control of Pratylenchus penetrans by aldicarb. Annals of Applied Biology 101:297-304.
2. Atkinson, H. J. 1976. The respiratory physiology of nematodes. Pp. 243-272 in N. A. Croll, ed. The organisation of nematodes. London: Academic Press.
3. Badhwar, R., R. K. Raheja, S. Ahuju, and S. P. Ahuju. 1995. Lipid composition of the seed gall nematode, Anguina tritici. Nematologica 41:584-591.
4. Christophers, A. E. P., M. N. Patel, J. A. Benson, V. Saka, A. A. F. Evans, and D. J. Wright. 1996. Rapid field-laboratory bioassay to assess the infectivity of Meloidogyne spp. Nematologica (in press).
5. Croll, N. A. 1972. Energy utilisation of infective Ancylostoma tubaeforme larvae. Parasitology 64:355-365.
6. Griffin, C. T., S. A. Joyce, I. Dix, A. M. Burnell, and M. J. Downes. 1994. Characterisation of the entomopathogenic nematode Heterorhabditis (Nematoda: Heterorhabditidae) from Ireland and Britain by molecular and cross-breeding techniques, and the occurrence of the genus in these islands. Fundamental and Applied Nematology 17:245-253.
7. Hendrikx, E. M. L., E. M. J. Meijer, and R. J. F. van Haren. 1994. A biochemical method for calibration of lipid determination techniques for potato cyst nematodes. p. 71 in Proceedings, 22nd International Symposium of the European Society of Nematologists, 7-12 August, Gent, Belgium.
8. Jung, K. 1991. Observations on the infective juveniles of the insect-parasitic nematode, Heterorhabditis sp., at two storage temperatures. Proceedings, International Symposium on Crop Protection, University of Gent, Belgium, 56/3b:1305-1312.
9. Lee, D. L., and H. J. Atkinson. 1976. Physiology of nematodes. 2nd ed. London: MacMillan Press.
10. Patel, M. N., M. Stolinski, and D. J. Wright. 1996. Neutral lipids and the assessment of infectivity in entomopathogenic nematodes: Observations on four Steinernema species. Parasitology (in press).
11. Reversat, G., Y. Demeure, B. Souchaud, and C. Paycheng. 1980. A photographic technique to evaluate the consumption of food reserves in individual starved second-stage juveniles of Meloidogyne javanica. Revue de Nématologie 3:101-105.
12. Robinson, M. P., H. P. Atkinson, and N. R. Perry. 1987. The influence of soil moisture and storage time on the motility, infectivity, and lipid utilization of second-stage juveniles of the potato cyst nematode Globodera rostochiensis and G. pallida. Revue de Nématologie 10:343-348.
13. Selvan, S., R. Gaugler, and P. S. Grewal. 1993a. Water content and fatty acid composition of infective juvenile entomopathogenic nematodes during storage. Journal of Parasitology 79:510-516.
14. Selvan, S., R. Gaugler, and E. E. Lewis, 1993b. Biochemical energy reserves of entomopathogenic nematodes. Journal of Parasitology 79:167-172.
15. Smits, P. H., J. T. M. Groenen, and G. de Raay. 1991. Characterization of Heterorhabditis isolates using DNA restriction length polymorphism. Revue de Nématologie 14:445-453.
16. Southey, J. F. 1970. Laboratory methods for work with plant and soil nematodes. Ministry of Agriculture, Fisheries and Food, Technical Bulletin 2. London: Her Majesty's Stationery Office.
17. Storey, R. M. J. 1983. The initial neutral lipid reserves of juveniles of Globodera spp. Nematologica 29:44-150.
18. Stryer, I. 1988. Biochemistry. 2nd ed., New York: Freeman.
19. Tiilikkala, K. A. 1991. Influence of soil temperature on initial energy reserves of Globodera rostochiensis larvae. Fundamental and Applied Nematology 15:49-54.
20. Van Gundy, S. D., A. F. Bird, and H. R. Wallace. 1967. Aging and starvation in larvae of Meloidogyne javanica and Tylenchulus semipenetrans. Phytopathology 57:559-571.
21. Vänninnen, I. 1990. Depletion of endogenous lipid reserves in Steinernema feltiae and Heterorhabditis bacteriophora and effect on infectivity. p. 132 in Proceedings of the Vth International Colloquium, Invertebrate Pathology and Microbial Control, 20-24 August 1990, Adelaide, Australia.
22. Westerman, P. R., and M. Stapel. 1992. Linear regression models describing the performance of the insect-parasitic nematode, Heterorhabditis sp., during storage. Fundamental and Applied Nematology 15:525-530.
23. Wright, D. J., F. T. J. Roberts, and A. A. F. Evans. 1989. Effects of the nematicide oxamyl on lipid utilisation and infectivity in Globodera rostochiensis. Parasitology 98:151-154.