The Function of eryBVII Gene is to Epimerize TDP-6-Deoxy-L-threo-D-glycero-4-hexulose in the Biosynthesis of Erythromycin A

Journal of Biochemistry and Molecular Biology

Volumn 32, Issue 1, 1999, Pages 72-75

  Kim, Wonyoung ^a   Kim, Choonkeun ^a   Han, Oksoo ^a  

^a Chonnam National University   (South Korea)

Author keywords

Biosynthesis; Deoxysugar; Erythromycin; TDP L mycarose

Indexed keywords

EID: 0033479409     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Blakeney, A. B., Harris, P. J., Henry, R. J. and Stone, B. A. (1983) A single and rapid preparation of alditol acetates for monosaccharide analysis. Carbohyd. Res. 113, 291-299.
2. Cortes, J., Haydock, S. F., Roberts, G. A., Bevitt, D. J. and Leadlay, P. F. (1990). An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythrea. Nature 348, 176-178.
3. Cupp-Vickery, J. R., Han, O., Hutchinson, C. R. and Poulos, T. L. (1996) Substrate assisted catalysis in cytochrome P450eryF. Nature: Struct. Biol. 3, 632-637.
4. Dhilson, N., Hale, R. S., Cortes, J. and Leadlay, P. F. (1989) Molecular characterization of a gene from Saccharopolyspora erythrea which is involved in erythromycin biosynthesis. Mol. Microbiol. 3, 1405-1414.
5. Gaisser, S., Bohm, G. A., Doumith, M., Raynal, M.-C, Dhilson, N., Cortes, J. and Leadlay, P. F. (1998) Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythrea. Mol. Gen. Genet. 258, 78-88.
6. Haydock, S. F., Dowson, J. A., Dhilson, N., Roberts, G. A., Cortes, J. and Leadlay, P. F. (1991) Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythrea: Sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol. Gen. Genet. 230, 120-128.
7. Jacobson, J. R., Hutchinson, C. R., Cane, D. E. and Khosla, C. (1997) Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase. Science 277, 367-369.
8. Lei, Y. (1995) Mechanistic Studies of Enzymes Involved in Biosynthesis of CDP-Ascarylose and TDP-Rhamnose, PhD Thesis, University of Minnesota, pp. 239-240.
9. Sohng, J. K. and Yoo, J. (1996) Cloning, sequencing and expression of dTDP-D-glucose-4,6-dehydratase gene from Streptpmyces antibioticus Tu99, a producer of chlorothricin. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) 29, 183-191.
10. Sohng, J. K., Oh, T. J. and Kim, C. G. (1998) Method for cloning and biosynthetic genes of secondary metabolites including deoxysugar from Actinomycetes. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) 31, 475-483.
11. Summers, R. G., Donadio, S., Staver, M. J., Wendt-Pienkowski, E., Hutchinson, C. R. and Katz, L. (1997) Characterization of ten genes from Saccharopolyspora erythrea that are involved in L-mycarose and D-desosamine production. Microbiology-UK, 143, 3251-3262.
12. Weigel, T. M., Miller, V. P. and Liu, H.-W. (1992) Mechanistic and stereochemical studies of a unique dehydration catalyzed by CDP-4-keto-6-deoxy-D-glucose-3-dehydrase: A pyridoxamine dependent enzyme isolated from Yersinia pseudotuberculosis. Biochemistry 31, 2140-2147.