Inhibition of β(s)-chain dependent polymerization by synergistic complementation of contact site perturbations of α-chain: Application of semisynthetic chimeric α-chains

Protein Engineering

Volumn 12, Issue 12, 1999, Pages 1105-1111

  Srinivasulu, Sonati ^a   Malavalli, Ashok ^a   Prabhakaran, Muthuchidambaran ^b   Nagel, Ronald L ^a   Acharya, A Seetharama ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b Structural Bioinformatics Inc   (United States)

Author keywords

Chimeric HbS; HbS polymerization; Intermolecular contact sites; Linkage map; Protein engineering; Super inhibitory chain

Indexed keywords

CHIMERIC PROTEIN; HEMOGLOBIN; HEMOGLOBIN S; OXYGEN; SYNTHETIC PEPTIDE;

ALPHA CHAIN; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BETA CHAIN; GENE THERAPY; GENETIC ENGINEERING; HORSE; HUMAN; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN POLYMERIZATION;

EID: 0033434101     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.12.1105     Document Type: Article

References (23)

1. Adachi, K. and Asakura, T. (1979) J. Biol. Chem., 254, 7765-7771.
2. Benesch, R.E., Kwong, S. and Benesch, R. (1982) Nature, 299, 231-234.
3. Bucci, E. (1981) Methods Enzymol., 76, 97-105.
4. Constantini, R. Chada, K. and Morgan, M.J. (1986) Science, 233, 1192-1194.
5. Craescu, C.T., Schaffer, C., Mispelter, J., Garin, J. and Rosa, J. (1986) J. Biol. Chem., 261, 7894-7901.
6. Cretegny, I. and Edelstein, S.J. (1993) J. Mol. Biol., 230, 733-738.
7. Fabry, M.E., Nagel, R.L., Pachnis, A., Suzuka, S.M. and Constantini, F. (1992) Proc. Natl Acad. Sci. USA, 89, 12150-12154.
8. Kraus, L.M., Miyaji, T, Iuchi, I. and Kraus, A.P. (1966) Biochemistry, 5, 3701-3708.
9. Nacharaju, P. and Acharya, A.S. (1992) Biochemistry, 31, 12673-12679.
10. Nacharaju, P., Roy, R.P., White, S.P., Nagel, R.L. and Acharya, A.S. (1997) J. Biol. Chem., 272, 27869-27876.
11. Padlan, E.A. and Love, W.E. (1985a) J. Biol. Chem., 260, 8272-8279.
12. Padlan, E.A. and Love W.E. (1985b) J. Biol. Chem., 260, 8280-8291.
13. Roy, R.P. and Acharya, A.S. (1994) Methods Enzymol., 231, 194-215.
14. Roy, R.P., Nagel, R.L. and Acharya, A.S. (1993) J. Biol. Chem., 268, 16406-16412.
15. Rhoda, M.D., Blouquit, Y., Martin, J.C., Monplaisir, N., Galacteros, F., Garel, M.C. and Rosa, J. (1984) Biochim. Biophys. Acta, 786, 62-68.
16. Rhoda, M., Domenget, C., Vidaud, M., Bardakdjian-Michau, J., Rouyer-Fessard, P., Rosa, J. and Beuzard, Y. (1988) Biochim. Biophys. Acta, 952, 208-212.
17. Russu, I.M. and Ho, C. (1980) Proc. Natl Acad. Sci. USA, 77, 6577-6581.
18. Sahni, G., Cho, Y.J., Iyer, K.S., Khan, S.A., Seetharam, R. and Acharya, A.S. (1989) Biochemistry, 28, 5456-5461.
19. Sellaye, M., Blouquit, Y., Galacteros, F., Arous, N., Monplaisir, N., Rhoda, M.D., Branconnier, F. and Rosa, J. (1982) FEBS Lett., 145, 128-130.
20. Sun, D.P., Zou, M., Ho, N.T. and Ho, C. (1997) Biochemistry, 36, 6663-6673.
21. Watowich, S.J., Gross, L.J. and Joseph, R. (1993) J. Struct. Biol., 111, 161-179.
22. Wishner, B.C., Ward, K.B., Lattman, E.E. and Love, W.E. (1975) J. Mol. Biol., 98, 179-194.
23. Yip, Y.K., Waks, M. and Beychok, S. (1977) Proc. Natl Acad. Sci. USA, 74, 64-68.