Real-Time Measurement of Fluorescence Spectra from Single Airborne Biological Particles

Field Analytical Chemistry and Technology

Volumn 3, Issue 4-5, 1999, Pages 221-239

  Hill, Steven C ^a   Pinnick, Ronald G ^a   Niles, Stanley ^a   Pan, Yong Le ^b   Holler, Stephen ^c   Chang, Richard K ^c   Bottiger, Jerold ^d   Chen, Bean T ^e   Orr, Chun Sing ^e   Feather, Greg ^e  

^a U S ARMY RESEARCH LABORATORY   (United States)

^b NEW MEXICO STATE UNIVERSITY   (United States)

^c Yale University   (United States)

^d EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

^e NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH   (United States)

Author keywords

Aerosol detection; Bioaerosols; Biological aerosols; Fluorescence spectroscopy; Instrumentation; Single particle fluorescence

Indexed keywords

BACILLUS SUBTILIS; GALLUS GALLUS;

EID: 0033433483     PISSN: 1086900X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1520-6521(1999)3:4/5<221::aid-fact2>3.0.co;2-7     Document Type: Article

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58. The ICCD camera (Princeton Instruments) is placed at the exit port of the spectrograph (Acton model SP-150 with 300 groove/mm grating blazed at 500 nm, numerical aperture 0.125, input slit width 1 mm). The ICCD detector's image intensifier acts as a fast shutter, opening when the targeted particle is illuminated by the UV laser. A long-pass filter is placed in front of the spectrograph to block elastically scattered light and to pass the fluorescence.
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